Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth
Objective This study aims to evaluate the potential of BST2-targeted microbubble-facilitated HIFU therapy for prostate cancer (PCa), and to elucidate the mechanisms of synergistic tumor suppression in residual lesions.Methods The specificity of BST-2 expression in PCa tumor endothelial cells was est...
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Taylor & Francis Group
2025-12-01
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| Series: | International Journal of Hyperthermia |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2025.2511035 |
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| author | Hang Zhou Jia Tang Yujie Wan Jiaqi Gong Qizhi Wang Kaifeng Huang Ruixia Hong Xinzhi Xu Fang Li |
| author_facet | Hang Zhou Jia Tang Yujie Wan Jiaqi Gong Qizhi Wang Kaifeng Huang Ruixia Hong Xinzhi Xu Fang Li |
| author_sort | Hang Zhou |
| collection | DOAJ |
| description | Objective This study aims to evaluate the potential of BST2-targeted microbubble-facilitated HIFU therapy for prostate cancer (PCa), and to elucidate the mechanisms of synergistic tumor suppression in residual lesions.Methods The specificity of BST-2 expression in PCa tumor endothelial cells was established using a cellular co-culture method. The targeting efficacy of Anti-BST-2 antibody-conjugated microbubbles (BST-2-MB) was evaluated both in vitro and in vivo. The therapeutic outcomes of BST-2-MB-augmented HIFU (High-Intensity Focused Ultrasound) for PCa in Balb/c mice were compared with those of non-targeted microbubbles and HIFU alone. Changes in the immunological microenvironment and modulation of BST-2-related oncogenic pathways in residual tumors were analyzed using Western Blot, immunofluorescence, flow cytometry, and ELISA.Results BST-2 is highly expressed in PCa vascular endothelial cells, and BST-2-MBs show strong targeting efficacy. HIFU combined with BST-2-MB achieved larger ablation volumes and potent inhibition of residual tumor growth. This treatment induced more immunogenic cell death (ICD), DC maturation, and CD8+ T cell infiltration, and significantly downregulated the expression of BST-2, EGFR, pSTAT3, and pErk1/2 compared to other treatments.Conclusion BST-2-MB combined with HIFU therapy demonstrates a superior synergistic antitumor effect in PCa, effectively inhibiting residual tumors, which offers a novel strategy to enhance HIFU efficacy. |
| format | Article |
| id | doaj-art-6aad4737cffd46729cb3e0fc8e46df03 |
| institution | DOAJ |
| issn | 0265-6736 1464-5157 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj-art-6aad4737cffd46729cb3e0fc8e46df032025-08-20T03:12:54ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572025-12-0142110.1080/02656736.2025.2511035Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growthHang Zhou0Jia Tang1Yujie Wan2Jiaqi Gong3Qizhi Wang4Kaifeng Huang5Ruixia Hong6Xinzhi Xu7Fang Li8Department of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaDepartment of Ultrasound, Chongqing University Cancer Hospital, Chongqing, ChinaObjective This study aims to evaluate the potential of BST2-targeted microbubble-facilitated HIFU therapy for prostate cancer (PCa), and to elucidate the mechanisms of synergistic tumor suppression in residual lesions.Methods The specificity of BST-2 expression in PCa tumor endothelial cells was established using a cellular co-culture method. The targeting efficacy of Anti-BST-2 antibody-conjugated microbubbles (BST-2-MB) was evaluated both in vitro and in vivo. The therapeutic outcomes of BST-2-MB-augmented HIFU (High-Intensity Focused Ultrasound) for PCa in Balb/c mice were compared with those of non-targeted microbubbles and HIFU alone. Changes in the immunological microenvironment and modulation of BST-2-related oncogenic pathways in residual tumors were analyzed using Western Blot, immunofluorescence, flow cytometry, and ELISA.Results BST-2 is highly expressed in PCa vascular endothelial cells, and BST-2-MBs show strong targeting efficacy. HIFU combined with BST-2-MB achieved larger ablation volumes and potent inhibition of residual tumor growth. This treatment induced more immunogenic cell death (ICD), DC maturation, and CD8+ T cell infiltration, and significantly downregulated the expression of BST-2, EGFR, pSTAT3, and pErk1/2 compared to other treatments.Conclusion BST-2-MB combined with HIFU therapy demonstrates a superior synergistic antitumor effect in PCa, effectively inhibiting residual tumors, which offers a novel strategy to enhance HIFU efficacy.https://www.tandfonline.com/doi/10.1080/02656736.2025.2511035HIFUultrasound molecular imagingmicrobubblesprostate cancerBST-2 |
| spellingShingle | Hang Zhou Jia Tang Yujie Wan Jiaqi Gong Qizhi Wang Kaifeng Huang Ruixia Hong Xinzhi Xu Fang Li Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth International Journal of Hyperthermia HIFU ultrasound molecular imaging microbubbles prostate cancer BST-2 |
| title | Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth |
| title_full | Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth |
| title_fullStr | Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth |
| title_full_unstemmed | Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth |
| title_short | Platform-based BST-2-targeted microbubbles enhance HIFU therapy and effectively inhibit prostate cancer residual growth |
| title_sort | platform based bst 2 targeted microbubbles enhance hifu therapy and effectively inhibit prostate cancer residual growth |
| topic | HIFU ultrasound molecular imaging microbubbles prostate cancer BST-2 |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2025.2511035 |
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