Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens

Clade 2.3.4.4 Eurasian lineage H5Nx highly pathogenic avian influenza virus (HPAIV) has become the globally dominant clade and caused global outbreaks since 2014. The clade 2.3.4.4 viruses have evolved into eight hemagglutinin subgroups (2.3.4.4a-h). In this study, we evaluated the infectivity, path...

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Main Authors: Jung-Hoon Kwon, Kateri Bertran, Dong-Hun Lee, Miria Ferreira Criado, Lindsay Killmaster, Mary J. Pantin-Jackwood, David E. Swayne
Format: Article
Language:English
Published: Taylor & Francis Group 2023-12-01
Series:Emerging Microbes and Infections
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Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2023.2218945
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author Jung-Hoon Kwon
Kateri Bertran
Dong-Hun Lee
Miria Ferreira Criado
Lindsay Killmaster
Mary J. Pantin-Jackwood
David E. Swayne
author_facet Jung-Hoon Kwon
Kateri Bertran
Dong-Hun Lee
Miria Ferreira Criado
Lindsay Killmaster
Mary J. Pantin-Jackwood
David E. Swayne
author_sort Jung-Hoon Kwon
collection DOAJ
description Clade 2.3.4.4 Eurasian lineage H5Nx highly pathogenic avian influenza virus (HPAIV) has become the globally dominant clade and caused global outbreaks since 2014. The clade 2.3.4.4 viruses have evolved into eight hemagglutinin subgroups (2.3.4.4a-h). In this study, we evaluated the infectivity, pathobiology, and transmissibility of seven clade 2.3.4.4 viruses (two 2.3.4.4a, two 2.3.4.4b, one 2.3.4.4c and two 2.3.4.4e) in chickens. The two clade 2.3.4.4e viruses caused 100% mortality and transmissibility in chickens. However, clade 2.3.4.4a and c viruses showed 80–90% mortality and 67% transmissibility. Clade 2.3.4.4b viruses showed 100% mortality, but no transmission to co-housed chickens was observed based on lack of seroconversion. All the infected chickens died showing systemic infection, irrespective of subgroup. The results highlight that all the clade 2.3.4.4 HPAIVs used in this study caused high mortality in infected chickens, but the transmissibility of the viruses in chickens was variable in contrast to that of previous Eurasian-lineage H5N1 HPAIVs. Changes in the pathogenicity and transmissibility of clade 2.3.4.4 HPAIVs warrant careful monitoring of the viruses to establish effective control strategies.
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spelling doaj-art-6aa6bb8bb5e142c281d2e5412280103c2025-08-20T03:52:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2218945Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickensJung-Hoon Kwon0Kateri Bertran1Dong-Hun Lee2Miria Ferreira Criado3Lindsay Killmaster4Mary J. Pantin-Jackwood5David E. Swayne6Agricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USAAgricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USACollege of Veterinary Medicine, Konkuk University, Seoul, Republic of KoreaAgricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USAAgricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USAAgricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USAAgricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA, USAClade 2.3.4.4 Eurasian lineage H5Nx highly pathogenic avian influenza virus (HPAIV) has become the globally dominant clade and caused global outbreaks since 2014. The clade 2.3.4.4 viruses have evolved into eight hemagglutinin subgroups (2.3.4.4a-h). In this study, we evaluated the infectivity, pathobiology, and transmissibility of seven clade 2.3.4.4 viruses (two 2.3.4.4a, two 2.3.4.4b, one 2.3.4.4c and two 2.3.4.4e) in chickens. The two clade 2.3.4.4e viruses caused 100% mortality and transmissibility in chickens. However, clade 2.3.4.4a and c viruses showed 80–90% mortality and 67% transmissibility. Clade 2.3.4.4b viruses showed 100% mortality, but no transmission to co-housed chickens was observed based on lack of seroconversion. All the infected chickens died showing systemic infection, irrespective of subgroup. The results highlight that all the clade 2.3.4.4 HPAIVs used in this study caused high mortality in infected chickens, but the transmissibility of the viruses in chickens was variable in contrast to that of previous Eurasian-lineage H5N1 HPAIVs. Changes in the pathogenicity and transmissibility of clade 2.3.4.4 HPAIVs warrant careful monitoring of the viruses to establish effective control strategies.https://www.tandfonline.com/doi/10.1080/22221751.2023.2218945Avian influenza virushighly pathogenic avian influenza virusclade 2.3.4.4infectivitypathogenicitytransmissibility
spellingShingle Jung-Hoon Kwon
Kateri Bertran
Dong-Hun Lee
Miria Ferreira Criado
Lindsay Killmaster
Mary J. Pantin-Jackwood
David E. Swayne
Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
Emerging Microbes and Infections
Avian influenza virus
highly pathogenic avian influenza virus
clade 2.3.4.4
infectivity
pathogenicity
transmissibility
title Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
title_full Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
title_fullStr Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
title_full_unstemmed Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
title_short Diverse infectivity, transmissibility, and pathobiology of clade 2.3.4.4 H5Nx highly pathogenic avian influenza viruses in chickens
title_sort diverse infectivity transmissibility and pathobiology of clade 2 3 4 4 h5nx highly pathogenic avian influenza viruses in chickens
topic Avian influenza virus
highly pathogenic avian influenza virus
clade 2.3.4.4
infectivity
pathogenicity
transmissibility
url https://www.tandfonline.com/doi/10.1080/22221751.2023.2218945
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