Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6)
Studies on populations exposed to inorganic arsenic (iAs) have shown an association with the development of chronic kidney disease (CKD) and renal cell carcinoma (RCC). However, there are few studies addressing how acute exposure of the human kidney to iAs might lead to the long-term alterations tha...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/6/455 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850156243995328512 |
|---|---|
| author | Md Ehsanul Haque Donald A. Sens Scott H. Garrett |
| author_facet | Md Ehsanul Haque Donald A. Sens Scott H. Garrett |
| author_sort | Md Ehsanul Haque |
| collection | DOAJ |
| description | Studies on populations exposed to inorganic arsenic (iAs) have shown an association with the development of chronic kidney disease (CKD) and renal cell carcinoma (RCC). However, there are few studies addressing how acute exposure of the human kidney to iAs might lead to the long-term alterations that might lead to CKD or RCC. This laboratory’s hypothesis is that renal exposure to iAs might alter the renal cells responsible for the repair and regeneration of nephrons damaged by iAs exposure or other renal toxicants. The kidney possesses a minority epithelial cell population that co-expresses PROM1 and CD24, which are believed to be involved in renal epithelial cell repair. The purpose of this work is to understand the pathogenesis of CKD in renal cortical epithelial cells. Our model consists of acute and chronic exposure of i-As (III) to “Human Renal Tubular Precursor TERT” (HRTPT). The microarray and gene validation study demonstrated a sudden induction of microfibril associated protein 5 (MFAP5) and carcinoembryonic antigen related cell adhesion molecule 5 and 6 (CEACAM 5 and 6) in chronic i-As (III)-exposed cells. Chronically exposed cells also exhibited an induction of the pAKT/AKT pathway and SOX9 transcription factor. The targeting of MFAP5 and CEACAM 5/6 could, therefore, provide a potential therapeutic approach to CKD. |
| format | Article |
| id | doaj-art-6a9570810fe24de286266d4edc4d1aa4 |
| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-6a9570810fe24de286266d4edc4d1aa42025-08-20T02:24:37ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-06-0147645510.3390/cimb47060455Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6)Md Ehsanul Haque0Donald A. Sens1Scott H. Garrett2Department of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202, USADepartment of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202, USADepartment of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202, USAStudies on populations exposed to inorganic arsenic (iAs) have shown an association with the development of chronic kidney disease (CKD) and renal cell carcinoma (RCC). However, there are few studies addressing how acute exposure of the human kidney to iAs might lead to the long-term alterations that might lead to CKD or RCC. This laboratory’s hypothesis is that renal exposure to iAs might alter the renal cells responsible for the repair and regeneration of nephrons damaged by iAs exposure or other renal toxicants. The kidney possesses a minority epithelial cell population that co-expresses PROM1 and CD24, which are believed to be involved in renal epithelial cell repair. The purpose of this work is to understand the pathogenesis of CKD in renal cortical epithelial cells. Our model consists of acute and chronic exposure of i-As (III) to “Human Renal Tubular Precursor TERT” (HRTPT). The microarray and gene validation study demonstrated a sudden induction of microfibril associated protein 5 (MFAP5) and carcinoembryonic antigen related cell adhesion molecule 5 and 6 (CEACAM 5 and 6) in chronic i-As (III)-exposed cells. Chronically exposed cells also exhibited an induction of the pAKT/AKT pathway and SOX9 transcription factor. The targeting of MFAP5 and CEACAM 5/6 could, therefore, provide a potential therapeutic approach to CKD.https://www.mdpi.com/1467-3045/47/6/455arsenite (As III)HRTPTrenal fibrosisMFAP5CEACAM 5 and 6 |
| spellingShingle | Md Ehsanul Haque Donald A. Sens Scott H. Garrett Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) Current Issues in Molecular Biology arsenite (As III) HRTPT renal fibrosis MFAP5 CEACAM 5 and 6 |
| title | Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) |
| title_full | Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) |
| title_fullStr | Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) |
| title_full_unstemmed | Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) |
| title_short | Chronic Exposure of Renal Progenitor Cells (HRTPT) to As (III) Implicates Microfibril Associated Protein 5 (MFAP5) in the Activation of Carcinoembryonic Antigen Related Cell Adhesion Molecules (CEACAM 5 and 6) |
| title_sort | chronic exposure of renal progenitor cells hrtpt to as iii implicates microfibril associated protein 5 mfap5 in the activation of carcinoembryonic antigen related cell adhesion molecules ceacam 5 and 6 |
| topic | arsenite (As III) HRTPT renal fibrosis MFAP5 CEACAM 5 and 6 |
| url | https://www.mdpi.com/1467-3045/47/6/455 |
| work_keys_str_mv | AT mdehsanulhaque chronicexposureofrenalprogenitorcellshrtpttoasiiiimplicatesmicrofibrilassociatedprotein5mfap5intheactivationofcarcinoembryonicantigenrelatedcelladhesionmoleculesceacam5and6 AT donaldasens chronicexposureofrenalprogenitorcellshrtpttoasiiiimplicatesmicrofibrilassociatedprotein5mfap5intheactivationofcarcinoembryonicantigenrelatedcelladhesionmoleculesceacam5and6 AT scotthgarrett chronicexposureofrenalprogenitorcellshrtpttoasiiiimplicatesmicrofibrilassociatedprotein5mfap5intheactivationofcarcinoembryonicantigenrelatedcelladhesionmoleculesceacam5and6 |