Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems
The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory fa...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/7486279 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556189470162944 |
|---|---|
| author | Vitale Miceli Mariangela Pampalone Serena Vella Anna Paola Carreca Giandomenico Amico Pier Giulio Conaldi |
| author_facet | Vitale Miceli Mariangela Pampalone Serena Vella Anna Paola Carreca Giandomenico Amico Pier Giulio Conaldi |
| author_sort | Vitale Miceli |
| collection | DOAJ |
| description | The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). The regenerative potential of both 3D culture-derived conditioned medium (3D CM) and their exosomes (EXO) was assessed against 2D culture products. In particular, tubulogenesis assays revealed increased capillary maturation in the presence of 3D CM compared with both 2D CM and 2D EXO. Furthermore, 3D CM had a greater effect on inhibition of PBMC proliferation than both 2D CM and 2D EXO. To support this data, hAMSC spheroids kept in our 3D culture system remained viable and multipotent and secreted considerable amounts of both angiogenic and immunosuppressive factors, which were detected at lower levels in 2D cultures. This work reveals the placenta as an important source of MSCs that can be used for eventual clinical applications as cell-free therapies. |
| format | Article |
| id | doaj-art-6a934e7a86364222a85438798298d889 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6a934e7a86364222a85438798298d8892025-02-03T05:46:01ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/74862797486279Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture SystemsVitale Miceli0Mariangela Pampalone1Serena Vella2Anna Paola Carreca3Giandomenico Amico4Pier Giulio Conaldi5Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, ItalyDepartment of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, ItalyAnemocyte S.r.l., Gerenzano, ItalyRi.MED Foundation, Palermo, ItalyDepartment of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, ItalyDepartment of Research, IRCCS-ISMETT, Palermo, ItalyThe secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). The regenerative potential of both 3D culture-derived conditioned medium (3D CM) and their exosomes (EXO) was assessed against 2D culture products. In particular, tubulogenesis assays revealed increased capillary maturation in the presence of 3D CM compared with both 2D CM and 2D EXO. Furthermore, 3D CM had a greater effect on inhibition of PBMC proliferation than both 2D CM and 2D EXO. To support this data, hAMSC spheroids kept in our 3D culture system remained viable and multipotent and secreted considerable amounts of both angiogenic and immunosuppressive factors, which were detected at lower levels in 2D cultures. This work reveals the placenta as an important source of MSCs that can be used for eventual clinical applications as cell-free therapies.http://dx.doi.org/10.1155/2019/7486279 |
| spellingShingle | Vitale Miceli Mariangela Pampalone Serena Vella Anna Paola Carreca Giandomenico Amico Pier Giulio Conaldi Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems Stem Cells International |
| title | Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems |
| title_full | Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems |
| title_fullStr | Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems |
| title_full_unstemmed | Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems |
| title_short | Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems |
| title_sort | comparison of immunosuppressive and angiogenic properties of human amnion derived mesenchymal stem cells between 2d and 3d culture systems |
| url | http://dx.doi.org/10.1155/2019/7486279 |
| work_keys_str_mv | AT vitalemiceli comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems AT mariangelapampalone comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems AT serenavella comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems AT annapaolacarreca comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems AT giandomenicoamico comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems AT piergiulioconaldi comparisonofimmunosuppressiveandangiogenicpropertiesofhumanamnionderivedmesenchymalstemcellsbetween2dand3dculturesystems |