Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus
Coagulase-negative staphylococci (CNS), in particular Staphylococcus haemolyticus, play an important role in the etiology of nosocomial infections. Most CNS are resistant to beta-lactam antibiotics, which is realized through the production of the second penicillin-binding protein. This protein is en...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
LLC "Publishing House OKI"
2020-05-01
|
| Series: | Антибиотики и Химиотерапия |
| Subjects: | |
| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/94 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849244552604418048 |
|---|---|
| author | A. V. Polonskaya M. A. Kornienko A. I. Manolov N. S. Kuptsov G. B. Smirnov L. A. Lyubasovskaya T. V. Priputnevich E. A. Shitikov E. N. Ilina |
| author_facet | A. V. Polonskaya M. A. Kornienko A. I. Manolov N. S. Kuptsov G. B. Smirnov L. A. Lyubasovskaya T. V. Priputnevich E. A. Shitikov E. N. Ilina |
| author_sort | A. V. Polonskaya |
| collection | DOAJ |
| description | Coagulase-negative staphylococci (CNS), in particular Staphylococcus haemolyticus, play an important role in the etiology of nosocomial infections. Most CNS are resistant to beta-lactam antibiotics, which is realized through the production of the second penicillin-binding protein. This protein is encoded by the mecA gene and, together with the genes of recombinases (ccr), is part of the mobile element of staphylococci - the staphylococcal cassette chromosome. During the study, the mecA and ccr genes were analyzed using a collection of 142 genomes of S.haemolyticus. The mecA gene was detected in 117 genomes (82.4%) and had a pronounced conservation. Based on the analysis of recombinase gene sequences, it was established that 118 samples (83%) contain ccr, and 22 different combinations of the mecA and recombinase genes presence are described. The combination of ccrA4B4 was the most common for S.haemolyticus (25%). Type-specific primers were proposed, to assess the variability of recombinase genes, their performance was validated on 54 clinical isolates. |
| format | Article |
| id | doaj-art-6a8b6fe8ef144ed8a709228f5ff1b4f8 |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-6a8b6fe8ef144ed8a709228f5ff1b4f82025-08-20T03:59:08ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01637-8334093Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus HaemolyticusA. V. Polonskaya0M. A. Kornienko1A. I. Manolov2N. S. Kuptsov3G. B. Smirnov4L. A. Lyubasovskaya5T. V. Priputnevich6E. A. Shitikov7E. N. Ilina8Federal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyNational Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. KulakovaNational Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V. I. KulakovaFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyFederal Research & Clinical Center of Physical-Chemical Medicine of Federal Medical Biological AgencyCoagulase-negative staphylococci (CNS), in particular Staphylococcus haemolyticus, play an important role in the etiology of nosocomial infections. Most CNS are resistant to beta-lactam antibiotics, which is realized through the production of the second penicillin-binding protein. This protein is encoded by the mecA gene and, together with the genes of recombinases (ccr), is part of the mobile element of staphylococci - the staphylococcal cassette chromosome. During the study, the mecA and ccr genes were analyzed using a collection of 142 genomes of S.haemolyticus. The mecA gene was detected in 117 genomes (82.4%) and had a pronounced conservation. Based on the analysis of recombinase gene sequences, it was established that 118 samples (83%) contain ccr, and 22 different combinations of the mecA and recombinase genes presence are described. The combination of ccrA4B4 was the most common for S.haemolyticus (25%). Type-specific primers were proposed, to assess the variability of recombinase genes, their performance was validated on 54 clinical isolates.https://www.antibiotics-chemotherapy.ru/jour/article/view/94s.haemolyticussccmeccoagulase-negative staphylococcis.haemolyticussccmecresistance to ß-lactam antibiotics |
| spellingShingle | A. V. Polonskaya M. A. Kornienko A. I. Manolov N. S. Kuptsov G. B. Smirnov L. A. Lyubasovskaya T. V. Priputnevich E. A. Shitikov E. N. Ilina Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus Антибиотики и Химиотерапия s.haemolyticus sccmec coagulase-negative staphylococci s.haemolyticus sccmec resistance to ß-lactam antibiotics |
| title | Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus |
| title_full | Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus |
| title_fullStr | Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus |
| title_full_unstemmed | Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus |
| title_short | Variability of Recombinase Genes and Staphylococcal Cassette Chromosome mecA of Staphylococcus Haemolyticus |
| title_sort | variability of recombinase genes and staphylococcal cassette chromosome meca of staphylococcus haemolyticus |
| topic | s.haemolyticus sccmec coagulase-negative staphylococci s.haemolyticus sccmec resistance to ß-lactam antibiotics |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/94 |
| work_keys_str_mv | AT avpolonskaya variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT makornienko variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT aimanolov variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT nskuptsov variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT gbsmirnov variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT lalyubasovskaya variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT tvpriputnevich variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT eashitikov variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus AT enilina variabilityofrecombinasegenesandstaphylococcalcassettechromosomemecaofstaphylococcushaemolyticus |