Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide

This study aimed to evaluate the influence of ultrasonic degradation on Houttuynia cordata polysaccharide (HCP) physicochemical properties, structure characterization, and bioactivities. The results indicated that the ultrasonic degradation could significantly decrease HCP’s molecular weight (MW). T...

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Main Authors: Mohammed Mansour, Ramy M. Khoder, Lin Xiang, Lan Lan Zhang, Ahmed Taha, Alsadig Yahya, Ting Wu, Hassan Barakat, Ibrahim Khalifa, Xu Xiaoyun
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Ultrasonics Sonochemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S1350417725001105
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author Mohammed Mansour
Ramy M. Khoder
Lin Xiang
Lan Lan Zhang
Ahmed Taha
Alsadig Yahya
Ting Wu
Hassan Barakat
Ibrahim Khalifa
Xu Xiaoyun
author_facet Mohammed Mansour
Ramy M. Khoder
Lin Xiang
Lan Lan Zhang
Ahmed Taha
Alsadig Yahya
Ting Wu
Hassan Barakat
Ibrahim Khalifa
Xu Xiaoyun
author_sort Mohammed Mansour
collection DOAJ
description This study aimed to evaluate the influence of ultrasonic degradation on Houttuynia cordata polysaccharide (HCP) physicochemical properties, structure characterization, and bioactivities. The results indicated that the ultrasonic degradation could significantly decrease HCP’s molecular weight (MW). Total polysaccharide, uronic acid content, solubility, and thermal stability of HCP increased gradually with the increase in ultrasonication power. Fourier transform infrared (FTIR) and Nuclear magnetic resonance spectroscopy (NMR) spectra proved that the primary structure of HCP had not been changed via ultrasonic degradation. Antioxidant and hypoglycemic activity results confirmed that ultrasonication enhanced the ability to scavenge free radicals (DPPH, ABTS, and OH) and improved α-glycosidase and α-amylase inhibition with the increase of ultrasonic power, which was increased in order HCP <U200 < U400 < U600. The degraded HCP produced via 600 W presented the best physicochemical properties and bioactivities. U600, α-amylase and α-glycosidase inhibition activities were 43.80 ± 0.68 and 83.28 ± 2.56 %, which were higher than those of native HCP 38.40 ± 0.53 and 65.67 ± 0.54 %, respectively, at a concentration of 10 mg/mL HCP solution. These results suggested that ultrasonication could be used as a green method for polysaccharide degradation and showed potential application for enhancing polysaccharide bioactivities for functional foods and pharmaceutical applications.
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spelling doaj-art-6a809b0bca2344acbf6e0a3b3c9e5f932025-08-20T03:06:05ZengElsevierUltrasonics Sonochemistry1350-41772025-05-0111610733110.1016/j.ultsonch.2025.107331Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharideMohammed Mansour0Ramy M. Khoder1Lin Xiang2Lan Lan Zhang3Ahmed Taha4Alsadig Yahya5Ting Wu6Hassan Barakat7Ibrahim Khalifa8Xu Xiaoyun9College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, China; Desert Research Center (DRC), Matariya, Cairo, EgyptCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, ChinaDepartment of Food Science, Faculty of Agricultural, (Saba Basha), Alexandria University, Alexandria 21531, EgyptCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, ChinaDepartment of Food Science and Human Nutrition, College of Agriculture and Food, Qassim University, Buraydah 51452, Saudi ArabiaFood Technology Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, EgyptCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Food Science (Huazhong Agricultural University), Ministry of Education, China; Hubei Key Laboratory of Fruit &amp; Vegetable Processing &amp; Quality Control, Huazhong Agricultural University, Wuhan, 430070, China; Corresponding author.This study aimed to evaluate the influence of ultrasonic degradation on Houttuynia cordata polysaccharide (HCP) physicochemical properties, structure characterization, and bioactivities. The results indicated that the ultrasonic degradation could significantly decrease HCP’s molecular weight (MW). Total polysaccharide, uronic acid content, solubility, and thermal stability of HCP increased gradually with the increase in ultrasonication power. Fourier transform infrared (FTIR) and Nuclear magnetic resonance spectroscopy (NMR) spectra proved that the primary structure of HCP had not been changed via ultrasonic degradation. Antioxidant and hypoglycemic activity results confirmed that ultrasonication enhanced the ability to scavenge free radicals (DPPH, ABTS, and OH) and improved α-glycosidase and α-amylase inhibition with the increase of ultrasonic power, which was increased in order HCP <U200 < U400 < U600. The degraded HCP produced via 600 W presented the best physicochemical properties and bioactivities. U600, α-amylase and α-glycosidase inhibition activities were 43.80 ± 0.68 and 83.28 ± 2.56 %, which were higher than those of native HCP 38.40 ± 0.53 and 65.67 ± 0.54 %, respectively, at a concentration of 10 mg/mL HCP solution. These results suggested that ultrasonication could be used as a green method for polysaccharide degradation and showed potential application for enhancing polysaccharide bioactivities for functional foods and pharmaceutical applications.http://www.sciencedirect.com/science/article/pii/S1350417725001105Houttuynia cordata polysaccharideUltrasonicationStructure characterizationAntioxidant activityHypoglycemic activityPolyphenols
spellingShingle Mohammed Mansour
Ramy M. Khoder
Lin Xiang
Lan Lan Zhang
Ahmed Taha
Alsadig Yahya
Ting Wu
Hassan Barakat
Ibrahim Khalifa
Xu Xiaoyun
Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
Ultrasonics Sonochemistry
Houttuynia cordata polysaccharide
Ultrasonication
Structure characterization
Antioxidant activity
Hypoglycemic activity
Polyphenols
title Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
title_full Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
title_fullStr Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
title_full_unstemmed Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
title_short Effect of ultrasonic degradation on the physicochemical property, structure characterization, and bioactivity of Houttuynia cordata polysaccharide
title_sort effect of ultrasonic degradation on the physicochemical property structure characterization and bioactivity of houttuynia cordata polysaccharide
topic Houttuynia cordata polysaccharide
Ultrasonication
Structure characterization
Antioxidant activity
Hypoglycemic activity
Polyphenols
url http://www.sciencedirect.com/science/article/pii/S1350417725001105
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