Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair
Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/8740294 |
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| _version_ | 1850223069892706304 |
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| author | Katsunori Shiraishi Naosuke Kamei Shunsuke Takeuchi Shinobu Yanada Hisashi Mera Shigeyuki Wakitani Nobuo Adachi Mitsuo Ochi |
| author_facet | Katsunori Shiraishi Naosuke Kamei Shunsuke Takeuchi Shinobu Yanada Hisashi Mera Shigeyuki Wakitani Nobuo Adachi Mitsuo Ochi |
| author_sort | Katsunori Shiraishi |
| collection | DOAJ |
| description | Quality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of MYBL1 was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of RCAN2 and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of HLA-DRB1 was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of TMEM155 and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair. |
| format | Article |
| id | doaj-art-6a7e60114f244a4e9012bd04f1c3a3f7 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6a7e60114f244a4e9012bd04f1c3a3f72025-08-20T02:06:05ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/87402948740294Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage RepairKatsunori Shiraishi0Naosuke Kamei1Shunsuke Takeuchi2Shinobu Yanada3Hisashi Mera4Shigeyuki Wakitani5Nobuo Adachi6Mitsuo Ochi7Department of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, JapanJapan Tissue Engineering Co., Ltd., Gamagori, JapanJapan Tissue Engineering Co., Ltd., Gamagori, JapanUonuma Institute of Community Medicine, Niigata University Medical and Dental Hospital, Niigata, JapanDepartment of Health and Sports Sciences, Mukogawa Women’s University, Nishinomiya, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, JapanQuality evaluation of mesenchymal stem cells (MSCs) based on efficacy would be helpful for their clinical application. In this study, we aimed to find the factors of human bone marrow MSCs relating to cartilage repair. The expression profiles of humoral factors, messenger RNAs (mRNAs), and microRNAs (miRNAs) were analyzed in human bone marrow MSCs from five different donors. We investigated the correlations of these expression profiles with the capacity of the MSCs for proliferation, chondrogenic differentiation, and cartilage repair in vivo. The mRNA expression of MYBL1 was positively correlated with proliferation and cartilage differentiation. By contrast, the mRNA expression of RCAN2 and the protein expression of TIMP-1 and VEGF were negatively correlated with proliferation and cartilage differentiation. However, MSCs from all five donors had the capacity to promote cartilage repair in vivo regardless of their capacity for proliferation and cartilage differentiation. The mRNA expression of HLA-DRB1 was positively correlated with cartilage repair in vivo. Meanwhile, the mRNA expression of TMEM155 and expression of miR-486-3p, miR-148b, miR-93, and miR-320B were negatively correlated with cartilage repair. The expression analysis of these factors might help to predict the ability of bone marrow MSCs to promote cartilage repair.http://dx.doi.org/10.1155/2017/8740294 |
| spellingShingle | Katsunori Shiraishi Naosuke Kamei Shunsuke Takeuchi Shinobu Yanada Hisashi Mera Shigeyuki Wakitani Nobuo Adachi Mitsuo Ochi Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair Stem Cells International |
| title | Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair |
| title_full | Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair |
| title_fullStr | Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair |
| title_full_unstemmed | Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair |
| title_short | Quality Evaluation of Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair |
| title_sort | quality evaluation of human bone marrow mesenchymal stem cells for cartilage repair |
| url | http://dx.doi.org/10.1155/2017/8740294 |
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