Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants

Abstract The Bala group of plants, classified under the Malvaceae family, is acknowledged in the Ayurvedic Pharmacopoeia of India and is well-known for its medicinal uses, especially in boosting vitality and addressing neurological and cardiovascular conditions. The key genera of this group, such as...

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Main Authors: Satish Maurya, Geetika Sukhramani, Changyoung Lee, Sangjin Jo, Jinki Kim, Eun Ju Jeong, Nelson A. Zamora, Randall Garcia, Zhiyun Zhang, Sangho Choi, Ritesh Kumar Choudhary, Soo-Yong Kim
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Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-07744-6
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author Satish Maurya
Geetika Sukhramani
Changyoung Lee
Sangjin Jo
Jinki Kim
Eun Ju Jeong
Nelson A. Zamora
Randall Garcia
Zhiyun Zhang
Sangho Choi
Ritesh Kumar Choudhary
Soo-Yong Kim
author_facet Satish Maurya
Geetika Sukhramani
Changyoung Lee
Sangjin Jo
Jinki Kim
Eun Ju Jeong
Nelson A. Zamora
Randall Garcia
Zhiyun Zhang
Sangho Choi
Ritesh Kumar Choudhary
Soo-Yong Kim
author_sort Satish Maurya
collection DOAJ
description Abstract The Bala group of plants, classified under the Malvaceae family, is acknowledged in the Ayurvedic Pharmacopoeia of India and is well-known for its medicinal uses, especially in boosting vitality and addressing neurological and cardiovascular conditions. The key genera of this group, such as Sida and Abutilon, are highly valued in traditional medicine; however, the efficacy of herbal formulations is frequently compromised by adulterants from morphologically similar genera like Malvastrum and Malva. To address this critical challenge, our study focuses on plastome sequencing of various Bala group taxa and their adulterants, aiming to delineate DNA signatures for key species and provide a robust strategy against adulteration. Comprehensive analyses of plastomes from Sida, Abutilon, Malvastrum, and Malva reveal distinct indels, simple sequence repeats (SSRs), and tandem repeats across coding and non-coding regions, as well as potential DNA barcodes within specific intergenic regions. Investigations into gene composition, genetic variations, and nucleotide diversity further elucidate phylogenetic relationships within the Malveae tribe. Phylogenomic analyses clarify the placement of the Bala group and its adulterants, offering robust insights into their evolutionary context. This study underscores the utility of plastome-based approaches in safeguarding the authenticity of Bala group herbal drugs and highlights the broader implications for quality assurance in traditional medicine.
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spelling doaj-art-6a7a047aedc546f1af746dc75e65d32a2025-08-20T03:05:22ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-07744-6Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterantsSatish Maurya0Geetika Sukhramani1Changyoung Lee2Sangjin Jo3Jinki Kim4Eun Ju Jeong5Nelson A. Zamora6Randall Garcia7Zhiyun Zhang8Sangho Choi9Ritesh Kumar Choudhary10Soo-Yong Kim11Agharkar Research InstituteAgharkar Research InstituteInternational Biological Material Research Center, Korea Research Institute of Bioscience and BiotechnologyInternational Biological Material Research Center, Korea Research Institute of Bioscience and BiotechnologyCustomer Support Team, Sejong National ArboretumDepartment of Plant and Biomaterials Science, Gyeongsang National UniversityNational Biodiversity Institute (INBio)National Biodiversity Institute (INBio)State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of SciencesInternational Biological Material Research Center, Korea Research Institute of Bioscience and BiotechnologyAgharkar Research InstituteInternational Biological Material Research Center, Korea Research Institute of Bioscience and BiotechnologyAbstract The Bala group of plants, classified under the Malvaceae family, is acknowledged in the Ayurvedic Pharmacopoeia of India and is well-known for its medicinal uses, especially in boosting vitality and addressing neurological and cardiovascular conditions. The key genera of this group, such as Sida and Abutilon, are highly valued in traditional medicine; however, the efficacy of herbal formulations is frequently compromised by adulterants from morphologically similar genera like Malvastrum and Malva. To address this critical challenge, our study focuses on plastome sequencing of various Bala group taxa and their adulterants, aiming to delineate DNA signatures for key species and provide a robust strategy against adulteration. Comprehensive analyses of plastomes from Sida, Abutilon, Malvastrum, and Malva reveal distinct indels, simple sequence repeats (SSRs), and tandem repeats across coding and non-coding regions, as well as potential DNA barcodes within specific intergenic regions. Investigations into gene composition, genetic variations, and nucleotide diversity further elucidate phylogenetic relationships within the Malveae tribe. Phylogenomic analyses clarify the placement of the Bala group and its adulterants, offering robust insights into their evolutionary context. This study underscores the utility of plastome-based approaches in safeguarding the authenticity of Bala group herbal drugs and highlights the broader implications for quality assurance in traditional medicine.https://doi.org/10.1038/s41598-025-07744-6Herbal drugsBala groupDNA superbarcodeSidaAbutilonMalvastrum
spellingShingle Satish Maurya
Geetika Sukhramani
Changyoung Lee
Sangjin Jo
Jinki Kim
Eun Ju Jeong
Nelson A. Zamora
Randall Garcia
Zhiyun Zhang
Sangho Choi
Ritesh Kumar Choudhary
Soo-Yong Kim
Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
Scientific Reports
Herbal drugs
Bala group
DNA superbarcode
Sida
Abutilon
Malvastrum
title Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
title_full Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
title_fullStr Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
title_full_unstemmed Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
title_short Comparative plastome evaluation, phylogenomic analysis, and DNA signatures of medicinally important malvaceous genera and their adulterants
title_sort comparative plastome evaluation phylogenomic analysis and dna signatures of medicinally important malvaceous genera and their adulterants
topic Herbal drugs
Bala group
DNA superbarcode
Sida
Abutilon
Malvastrum
url https://doi.org/10.1038/s41598-025-07744-6
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