Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection
Jiawei Ermiao Granule (JWEMG) is a traditional Chinese herbal formulation widely used in China for the treatment of human papilloma virus (HPV) infections. However, the mechanisms underlying their efficacy in clearing HPV infections remain unclear. This study aimed to elucidate the mechanisms by whi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1550908/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849728919391961088 |
|---|---|
| author | Xiu Li Xiu Li Li Ning Li Ning Hongting Zhao Yating Xu Yating Xu Yu Si Yu Si Jiayi Hua Qingling Ren Qingling Ren |
| author_facet | Xiu Li Xiu Li Li Ning Li Ning Hongting Zhao Yating Xu Yating Xu Yu Si Yu Si Jiayi Hua Qingling Ren Qingling Ren |
| author_sort | Xiu Li |
| collection | DOAJ |
| description | Jiawei Ermiao Granule (JWEMG) is a traditional Chinese herbal formulation widely used in China for the treatment of human papilloma virus (HPV) infections. However, the mechanisms underlying their efficacy in clearing HPV infections remain unclear. This study aimed to elucidate the mechanisms by which JWEMG clears persistent HPV infections from a metabolomics perspective using modern analytical techniques. Untargeted and targeted metabolomics analyses were performed on vaginal lavage samples from 33 patients using liquid chromatograph mass spectrometer (LC-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS). Untargeted metabolomics identified 47 potential biomarkers through volcano plot analysis, among which 30 exhibited a reversal trend following JWEMG intervention. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that JWEMG may exert therapeutic effects on patients with persistent HPV infections via pathways related to starch and sucrose metabolism, galactose metabolism, fructose and mannose metabolism, and amino sugar and nucleotide sugar metabolism. Targeted metabolomics revealed a significant increase in tyrosine levels in the vaginal/cervical microenvironment following JWEMG treatment. By integrating targeted and untargeted metabolomics, this study provides a comprehensive exploration of the holistic effects of JWEMG on HPV-infected patients, addressing the challenges of scientifically explaining the pharmacological mechanisms of multi-component, multi-target traditional Chinese medicines. |
| format | Article |
| id | doaj-art-6a6d25ece9ab4ce8a158d804a8232f6b |
| institution | DOAJ |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-6a6d25ece9ab4ce8a158d804a8232f6b2025-08-20T03:09:24ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-04-011510.3389/fcimb.2025.15509081550908Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infectionXiu Li0Xiu Li1Li Ning2Li Ning3Hongting Zhao4Yating Xu5Yating Xu6Yu Si7Yu Si8Jiayi Hua9Qingling Ren10Qingling Ren11Jiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaThe First Clinical Medical School, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaThe First Clinical Medical School, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaThe First Clinical Medical School, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaDepartment of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaThe First Clinical Medical School, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiangsu Clinical Medicine Innovation Center for Obstetrics and Reproduction, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaDepartment of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu, ChinaJiawei Ermiao Granule (JWEMG) is a traditional Chinese herbal formulation widely used in China for the treatment of human papilloma virus (HPV) infections. However, the mechanisms underlying their efficacy in clearing HPV infections remain unclear. This study aimed to elucidate the mechanisms by which JWEMG clears persistent HPV infections from a metabolomics perspective using modern analytical techniques. Untargeted and targeted metabolomics analyses were performed on vaginal lavage samples from 33 patients using liquid chromatograph mass spectrometer (LC-MS) and hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS). Untargeted metabolomics identified 47 potential biomarkers through volcano plot analysis, among which 30 exhibited a reversal trend following JWEMG intervention. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that JWEMG may exert therapeutic effects on patients with persistent HPV infections via pathways related to starch and sucrose metabolism, galactose metabolism, fructose and mannose metabolism, and amino sugar and nucleotide sugar metabolism. Targeted metabolomics revealed a significant increase in tyrosine levels in the vaginal/cervical microenvironment following JWEMG treatment. By integrating targeted and untargeted metabolomics, this study provides a comprehensive exploration of the holistic effects of JWEMG on HPV-infected patients, addressing the challenges of scientifically explaining the pharmacological mechanisms of multi-component, multi-target traditional Chinese medicines.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1550908/fullJiawei Ermiao GranuleHPVuntargeted metabolomicstargeted metabolomicsamino acid metabolismtyrosine |
| spellingShingle | Xiu Li Xiu Li Li Ning Li Ning Hongting Zhao Yating Xu Yating Xu Yu Si Yu Si Jiayi Hua Qingling Ren Qingling Ren Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection Frontiers in Cellular and Infection Microbiology Jiawei Ermiao Granule HPV untargeted metabolomics targeted metabolomics amino acid metabolism tyrosine |
| title | Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection |
| title_full | Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection |
| title_fullStr | Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection |
| title_full_unstemmed | Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection |
| title_short | Targeted and untargeted metabolomics uncovering the effects of Jiawei Ermiao Granule on patients with persistent HPV infection |
| title_sort | targeted and untargeted metabolomics uncovering the effects of jiawei ermiao granule on patients with persistent hpv infection |
| topic | Jiawei Ermiao Granule HPV untargeted metabolomics targeted metabolomics amino acid metabolism tyrosine |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1550908/full |
| work_keys_str_mv | AT xiuli targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT xiuli targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT lining targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT lining targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT hongtingzhao targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT yatingxu targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT yatingxu targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT yusi targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT yusi targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT jiayihua targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT qinglingren targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection AT qinglingren targetedanduntargetedmetabolomicsuncoveringtheeffectsofjiaweiermiaogranuleonpatientswithpersistenthpvinfection |