Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line

Various novel curcuminoids (1–7) and pyrazole derivatives of curcuminoids (8–14) were synthesized and characterized using spectrum analysis. The compounds produced were evaluated for antiproliferative effects against the A549 lung cancer cell line. Compound 2 exhibited notable cytotoxicity at 10.38...

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Main Authors: Mustafa Q. Alderawy, Mustafa M. AL-Hakiem, Reham A. AL-Anssari, Rita S. Elias
Format: Article
Language:English
Published: Pensoft Publishers 2025-08-01
Series:Pharmacia
Online Access:https://pharmacia.pensoft.net/article/160847/download/pdf/
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author Mustafa Q. Alderawy
Mustafa M. AL-Hakiem
Reham A. AL-Anssari
Rita S. Elias
author_facet Mustafa Q. Alderawy
Mustafa M. AL-Hakiem
Reham A. AL-Anssari
Rita S. Elias
author_sort Mustafa Q. Alderawy
collection DOAJ
description Various novel curcuminoids (1–7) and pyrazole derivatives of curcuminoids (8–14) were synthesized and characterized using spectrum analysis. The compounds produced were evaluated for antiproliferative effects against the A549 lung cancer cell line. Compound 2 exhibited notable cytotoxicity at 10.38 μg/mL, whereas compounds 4, 5, and 7 showed considerable cytotoxicity, with IC₅₀ concentrations of 47.1, 23.4, and 82.4 μg/mL, respectively. Conversely, the other synthesized compounds exhibited only modest cytotoxicity. Moreover, in silico molecular docking analyses of EGFR (PDB ID: 4HJ0) demonstrated that compounds 2 and 6 displayed the lowest binding energies, −11.52 kcal/mol and −11.47 kcal/mol, respectively, indicating a higher affinity for the active pocket of the receptor, characterized by robust hydrogen bond interactions. Compound 12 had the highest binding energy (−8.42 kcal/mol) and the weakest affinity (KI = 670.04 nM). SwissADME analysis revealed satisfactory drug-likeness among all compounds, with no violations of Lipinski’s rules and modest synthetic accessibility. ProTox-II indicated tolerable oral toxicity (LD₅₀: 1300–4000 mg/kg), although elevated immunotoxicity scores imply the need for additional safety assessment. These data identify compound 2 as a promising candidate for future optimization and preclinical development.
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spelling doaj-art-6a56e30dc6cd4556b0053e307119de4f2025-08-20T03:04:45ZengPensoft PublishersPharmacia2603-557X2025-08-017211010.3897/pharmacia.72.e160847160847Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell lineMustafa Q. Alderawy0Mustafa M. AL-Hakiem1Reham A. AL-Anssari2Rita S. Elias3University of BasrahUniversity of BasrahUniversity of BasrahUniversity of BasrahVarious novel curcuminoids (1–7) and pyrazole derivatives of curcuminoids (8–14) were synthesized and characterized using spectrum analysis. The compounds produced were evaluated for antiproliferative effects against the A549 lung cancer cell line. Compound 2 exhibited notable cytotoxicity at 10.38 μg/mL, whereas compounds 4, 5, and 7 showed considerable cytotoxicity, with IC₅₀ concentrations of 47.1, 23.4, and 82.4 μg/mL, respectively. Conversely, the other synthesized compounds exhibited only modest cytotoxicity. Moreover, in silico molecular docking analyses of EGFR (PDB ID: 4HJ0) demonstrated that compounds 2 and 6 displayed the lowest binding energies, −11.52 kcal/mol and −11.47 kcal/mol, respectively, indicating a higher affinity for the active pocket of the receptor, characterized by robust hydrogen bond interactions. Compound 12 had the highest binding energy (−8.42 kcal/mol) and the weakest affinity (KI = 670.04 nM). SwissADME analysis revealed satisfactory drug-likeness among all compounds, with no violations of Lipinski’s rules and modest synthetic accessibility. ProTox-II indicated tolerable oral toxicity (LD₅₀: 1300–4000 mg/kg), although elevated immunotoxicity scores imply the need for additional safety assessment. These data identify compound 2 as a promising candidate for future optimization and preclinical development.https://pharmacia.pensoft.net/article/160847/download/pdf/
spellingShingle Mustafa Q. Alderawy
Mustafa M. AL-Hakiem
Reham A. AL-Anssari
Rita S. Elias
Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
Pharmacia
title Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
title_full Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
title_fullStr Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
title_full_unstemmed Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
title_short Comprehensive molecular docking, cytotoxicity, and ADMET analysis of documented curcuminoid derivatives on the A549 cell line
title_sort comprehensive molecular docking cytotoxicity and admet analysis of documented curcuminoid derivatives on the a549 cell line
url https://pharmacia.pensoft.net/article/160847/download/pdf/
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AT rehamaalanssari comprehensivemoleculardockingcytotoxicityandadmetanalysisofdocumentedcurcuminoidderivativesonthea549cellline
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