Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI

The near-complete genome sequence of a Citrobacter werkmanii UPMLSS01, isolated from the Kemena River in Bintulu, Sarawak, Malaysia, is presented. Remarkably, this bacterium exhibited resistance to beta-lactam despite no prior exposure, suggesting an innate resistance mechanism in an environment inc...

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Main Authors: Sui Sien Leong, Han Ming Gan
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Data in Brief
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352340925005992
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author Sui Sien Leong
Han Ming Gan
author_facet Sui Sien Leong
Han Ming Gan
author_sort Sui Sien Leong
collection DOAJ
description The near-complete genome sequence of a Citrobacter werkmanii UPMLSS01, isolated from the Kemena River in Bintulu, Sarawak, Malaysia, is presented. Remarkably, this bacterium exhibited resistance to beta-lactam despite no prior exposure, suggesting an innate resistance mechanism in an environment increasingly impacted by anthropogenic pressures. Genome sequencing was performed using both Illumina (2 × 150 bp paired-end) and Oxford Nanopore technologies, yielding a high-quality de novo assembly with a total length of 4994,936 base pairs, a GC content of 52 %, and an N50 of 3.5 Mb. The assembly comprises three contigs, none of which were identified as plasmid or mobile elements. Genomic analysis revealed that the strain belongs to sequence type 948, while also harboring the blaCMY gene presumably responsible for the beta-lactam resistance. Notably, this is the first genome of Citrobacter werkmanii reported from Borneo, Malaysia, underscoring a significant milestone that provides a robust baseline for future phylogenomic studies and molecular resistance profiling in both environmental and clinical settings.
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spelling doaj-art-6a54283f7c1d41ee9f1a88429b8bd8a72025-08-20T02:59:57ZengElsevierData in Brief2352-34092025-08-016111187510.1016/j.dib.2025.111875Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBISui Sien Leong0Han Ming Gan1Department of Animal Sciences and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Campus Bintulu Sarawak, Bintulu 97008, Sarawak, Malaysia; International Institute of Aquaculture and Aquatic Sciences (I-AQUAS), Universiti Putra Malaysia, 0150 Port Dickson, Negeri Sembilan, Malaysia; Corresponding author.Patriot Biotech Sdn Bhd, Bandar Sunway, 47500, MalaysiaThe near-complete genome sequence of a Citrobacter werkmanii UPMLSS01, isolated from the Kemena River in Bintulu, Sarawak, Malaysia, is presented. Remarkably, this bacterium exhibited resistance to beta-lactam despite no prior exposure, suggesting an innate resistance mechanism in an environment increasingly impacted by anthropogenic pressures. Genome sequencing was performed using both Illumina (2 × 150 bp paired-end) and Oxford Nanopore technologies, yielding a high-quality de novo assembly with a total length of 4994,936 base pairs, a GC content of 52 %, and an N50 of 3.5 Mb. The assembly comprises three contigs, none of which were identified as plasmid or mobile elements. Genomic analysis revealed that the strain belongs to sequence type 948, while also harboring the blaCMY gene presumably responsible for the beta-lactam resistance. Notably, this is the first genome of Citrobacter werkmanii reported from Borneo, Malaysia, underscoring a significant milestone that provides a robust baseline for future phylogenomic studies and molecular resistance profiling in both environmental and clinical settings.http://www.sciencedirect.com/science/article/pii/S2352340925005992CitrobacterNanoporeIlluminaBeta-lactamase
spellingShingle Sui Sien Leong
Han Ming Gan
Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
Data in Brief
Citrobacter
Nanopore
Illumina
Beta-lactamase
title Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
title_full Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
title_fullStr Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
title_full_unstemmed Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
title_short Dataset for the near-complete hybrid genome assembly of Citrobacter werkmanii UPMLSS01 isolated from Borneo river and insights into its native antibiotic resistance repertoireNCBI
title_sort dataset for the near complete hybrid genome assembly of citrobacter werkmanii upmlss01 isolated from borneo river and insights into its native antibiotic resistance repertoirencbi
topic Citrobacter
Nanopore
Illumina
Beta-lactamase
url http://www.sciencedirect.com/science/article/pii/S2352340925005992
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AT hanminggan datasetforthenearcompletehybridgenomeassemblyofcitrobacterwerkmaniiupmlss01isolatedfromborneoriverandinsightsintoitsnativeantibioticresistancerepertoirencbi