Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides
GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henane...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/713410 |
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| author | Nai-Dong Chen Nai-Fu Chen Jun Li Cai-Yun Cao Jin-Mei Wang He-Ping Huang |
| author_facet | Nai-Dong Chen Nai-Fu Chen Jun Li Cai-Yun Cao Jin-Mei Wang He-Ping Huang |
| author_sort | Nai-Dong Chen |
| collection | DOAJ |
| description | GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the rcor values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the rcor values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed. |
| format | Article |
| id | doaj-art-6a506d1982544c829cb9b287285163de |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-6a506d1982544c829cb9b287285163de2025-02-03T06:00:32ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/713410713410Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of PolysaccharidesNai-Dong Chen0Nai-Fu Chen1Jun Li2Cai-Yun Cao3Jin-Mei Wang4He-Ping Huang5College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, ChinaCollege of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaCollege of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, ChinaCollege of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, ChinaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, ChinaGC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the rcor values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the rcor values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed.http://dx.doi.org/10.1155/2015/713410 |
| spellingShingle | Nai-Dong Chen Nai-Fu Chen Jun Li Cai-Yun Cao Jin-Mei Wang He-Ping Huang Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides International Journal of Analytical Chemistry |
| title | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
| title_full | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
| title_fullStr | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
| title_full_unstemmed | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
| title_short | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
| title_sort | similarity evaluation of different origins and species of dendrobiums by gc ms and ftir analysis of polysaccharides |
| url | http://dx.doi.org/10.1155/2015/713410 |
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