Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study

Abstract Fine-Needle Aspiration (FNA) has been routinely used for papillary thyroid carcinoma (PTC) diagnosis. One single liquid based tissue sample collected from FNA can be used for both cytological diagnosis and genetic testing at the same time. BRAF V600E mutation exhibits 100% specificity and h...

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Main Authors: Hui Tang, Dan Guo, Bin Yang, Shu-hua Huang
Format: Article
Language:English
Published: BMC 2025-03-01
Series:BMC Cancer
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Online Access:https://doi.org/10.1186/s12885-025-13776-y
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author Hui Tang
Dan Guo
Bin Yang
Shu-hua Huang
author_facet Hui Tang
Dan Guo
Bin Yang
Shu-hua Huang
author_sort Hui Tang
collection DOAJ
description Abstract Fine-Needle Aspiration (FNA) has been routinely used for papillary thyroid carcinoma (PTC) diagnosis. One single liquid based tissue sample collected from FNA can be used for both cytological diagnosis and genetic testing at the same time. BRAF V600E mutation exhibits 100% specificity and high sensitivity for papillary thyroid carcinoma (PTC). However, FNA based studies on the genotypic (BRAF V600E) and ultrasonic (US) imaging characteristics (location and diameter) are exceedingly rare. Here, we aimed to study the genotypic (BRAF V600E) and US imaging characteristics (location and diameter) in a large retrospective cohort. A total of 4808 patients with thyroid nodules from a tertiary center underwent FNA, and 2430 patients underwent molecular testing. Our data demonstrated that the thyroid nodules were predominantly located on the right side (p = 0.0004). Patients diagnosed with Bethesda VI cytology had significantly more right-sided thyroid nodules (p = 0.0041). Interestingly, among patients with PTC with lymph node metastasis (LNM), right-side-affected LNM was significantly more common than left-side-affected LNM, which implies a biased regional LNM of right-side-located thyroid nodules (p = 0.0007). The size of BRAF-V600E mutated or right-lobe located nodules was significantly larger than that in the control group (p = 0.0156), and patients with a BRAF V600E mutation were considerably younger than those with wild-type BRAF.
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spelling doaj-art-6a4d783894314cdcbd06cf7e9b248c4d2025-08-20T01:57:45ZengBMCBMC Cancer1471-24072025-03-012511910.1186/s12885-025-13776-yLocation based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based studyHui Tang0Dan Guo1Bin Yang2Shu-hua Huang3Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityNorthern Jiangsu People’s Hospital Affiliated to Yangzhou UniversityNorthern Jiangsu People’s Hospital Affiliated to Yangzhou UniversityNorthern Jiangsu People’s Hospital Affiliated to Yangzhou UniversityAbstract Fine-Needle Aspiration (FNA) has been routinely used for papillary thyroid carcinoma (PTC) diagnosis. One single liquid based tissue sample collected from FNA can be used for both cytological diagnosis and genetic testing at the same time. BRAF V600E mutation exhibits 100% specificity and high sensitivity for papillary thyroid carcinoma (PTC). However, FNA based studies on the genotypic (BRAF V600E) and ultrasonic (US) imaging characteristics (location and diameter) are exceedingly rare. Here, we aimed to study the genotypic (BRAF V600E) and US imaging characteristics (location and diameter) in a large retrospective cohort. A total of 4808 patients with thyroid nodules from a tertiary center underwent FNA, and 2430 patients underwent molecular testing. Our data demonstrated that the thyroid nodules were predominantly located on the right side (p = 0.0004). Patients diagnosed with Bethesda VI cytology had significantly more right-sided thyroid nodules (p = 0.0041). Interestingly, among patients with PTC with lymph node metastasis (LNM), right-side-affected LNM was significantly more common than left-side-affected LNM, which implies a biased regional LNM of right-side-located thyroid nodules (p = 0.0007). The size of BRAF-V600E mutated or right-lobe located nodules was significantly larger than that in the control group (p = 0.0156), and patients with a BRAF V600E mutation were considerably younger than those with wild-type BRAF.https://doi.org/10.1186/s12885-025-13776-yFine needle aspirationPapillary thyroid carcinoma (PTC)BRAFTERTThyroid nodulesMaximal diameter
spellingShingle Hui Tang
Dan Guo
Bin Yang
Shu-hua Huang
Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
BMC Cancer
Fine needle aspiration
Papillary thyroid carcinoma (PTC)
BRAF
TERT
Thyroid nodules
Maximal diameter
title Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
title_full Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
title_fullStr Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
title_full_unstemmed Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
title_short Location based BRAF V600E mutation status and dimension patterns of sporadic thyroid nodules: a population-based study
title_sort location based braf v600e mutation status and dimension patterns of sporadic thyroid nodules a population based study
topic Fine needle aspiration
Papillary thyroid carcinoma (PTC)
BRAF
TERT
Thyroid nodules
Maximal diameter
url https://doi.org/10.1186/s12885-025-13776-y
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