Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825
Abstract Basil (Ocimum L.) is an important essential oil crop, medicinal plant, and culinary herb, belonging to the Lamiaceae family. It has extensive nutritional and therapeutic benefits, making it valuable in culinary and medicinal applications. Hence, in this study, we aimed to induce basil’s nut...
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2025-08-01
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| Online Access: | https://doi.org/10.1186/s12870-025-07082-9 |
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| author | Ahmed M. Mahmoud Maha H. Khalaf Ahmed M. Reyad Shereen Magdy Korany Emad A. Alsherif Hiba Shaghaleh Yousef Alhaj Hamoud Mohamed S. Sheteiwy Ali El-Keblawy Zaid Ulhassan |
| author_facet | Ahmed M. Mahmoud Maha H. Khalaf Ahmed M. Reyad Shereen Magdy Korany Emad A. Alsherif Hiba Shaghaleh Yousef Alhaj Hamoud Mohamed S. Sheteiwy Ali El-Keblawy Zaid Ulhassan |
| author_sort | Ahmed M. Mahmoud |
| collection | DOAJ |
| description | Abstract Basil (Ocimum L.) is an important essential oil crop, medicinal plant, and culinary herb, belonging to the Lamiaceae family. It has extensive nutritional and therapeutic benefits, making it valuable in culinary and medicinal applications. Hence, in this study, we aimed to induce basil’s nutritive and biological value. To this end, this study evaluates the potential of the plant growth-promoting Pseudomonas JP0825, isolated from the Jazan region, KSA, as a biopriming agent to improve the growth, nutritional quality, and bioactive compound profile of sweet (Ocimum basilicum L.) and American (Ocimum americanum L.) basil. The molecular identification of Pseudomonas JP0825 confirmed its phylogenetic relationship with other beneficial Pseudomonas species. Our findings revealed significant increases in photosynthetic pigments, biomass, and proximate composition, particularly in sweet basil, following inoculation. Elevated levels of vitamins, amino acids, and organic and fatty acids were observed, alongside enhanced secondary metabolites like phenolics and flavonoids, correlated with enhanced antioxidant and antimicrobial activity. The antioxidant properties of treated basil improved significantly, as indicated by increased FRAP and ABTS activities. Furthermore, Pseudomonas JP0825 demonstrated an ability to boost the antimicrobial activity against various pathogenic bacteria and fungi, including Staphylococcus epidermidis, Enterococcus faecalis, Salmonella typhimurium, and Aspergillus flavus. These findings highlight the strain’s potential as a sustainable alternative to chemical inputs, offering improvements in crop quality and resilience, and contributing to global food security efforts. |
| format | Article |
| id | doaj-art-6a4b30b1febf45b59f68ef5eafafa231 |
| institution | DOAJ |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-6a4b30b1febf45b59f68ef5eafafa2312025-08-20T03:04:23ZengBMCBMC Plant Biology1471-22292025-08-0125112210.1186/s12870-025-07082-9Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825Ahmed M. Mahmoud0Maha H. Khalaf1Ahmed M. Reyad2Shereen Magdy Korany3Emad A. Alsherif4Hiba Shaghaleh5Yousef Alhaj Hamoud6Mohamed S. Sheteiwy7Ali El-Keblawy8Zaid Ulhassan9Department of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversitySchool of Energy and Environment, Southeast University, Hohai UniversityThe National Key Laboratory of Water Disaster Prevention and College of Hydrology and Water Resources, Hohai UniversityDepartment of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates UniversityDepartment of Applied Biology, College of Sciences, University of SharjahInstitute of Crop Science, Ministry of Agriculture and Rural Affairs, Key Laboratory of Spectroscopy Sensing, Zhejiang UniversityAbstract Basil (Ocimum L.) is an important essential oil crop, medicinal plant, and culinary herb, belonging to the Lamiaceae family. It has extensive nutritional and therapeutic benefits, making it valuable in culinary and medicinal applications. Hence, in this study, we aimed to induce basil’s nutritive and biological value. To this end, this study evaluates the potential of the plant growth-promoting Pseudomonas JP0825, isolated from the Jazan region, KSA, as a biopriming agent to improve the growth, nutritional quality, and bioactive compound profile of sweet (Ocimum basilicum L.) and American (Ocimum americanum L.) basil. The molecular identification of Pseudomonas JP0825 confirmed its phylogenetic relationship with other beneficial Pseudomonas species. Our findings revealed significant increases in photosynthetic pigments, biomass, and proximate composition, particularly in sweet basil, following inoculation. Elevated levels of vitamins, amino acids, and organic and fatty acids were observed, alongside enhanced secondary metabolites like phenolics and flavonoids, correlated with enhanced antioxidant and antimicrobial activity. The antioxidant properties of treated basil improved significantly, as indicated by increased FRAP and ABTS activities. Furthermore, Pseudomonas JP0825 demonstrated an ability to boost the antimicrobial activity against various pathogenic bacteria and fungi, including Staphylococcus epidermidis, Enterococcus faecalis, Salmonella typhimurium, and Aspergillus flavus. These findings highlight the strain’s potential as a sustainable alternative to chemical inputs, offering improvements in crop quality and resilience, and contributing to global food security efforts.https://doi.org/10.1186/s12870-025-07082-9ActinobacteriaAntimicrobialAntioxidantBiofertilizerPrimary metabolites |
| spellingShingle | Ahmed M. Mahmoud Maha H. Khalaf Ahmed M. Reyad Shereen Magdy Korany Emad A. Alsherif Hiba Shaghaleh Yousef Alhaj Hamoud Mohamed S. Sheteiwy Ali El-Keblawy Zaid Ulhassan Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 BMC Plant Biology Actinobacteria Antimicrobial Antioxidant Biofertilizer Primary metabolites |
| title | Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 |
| title_full | Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 |
| title_fullStr | Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 |
| title_full_unstemmed | Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 |
| title_short | Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825 |
| title_sort | sustainable enhancement of basil quality and resilience through biopriming with pseudomonas jp0825 |
| topic | Actinobacteria Antimicrobial Antioxidant Biofertilizer Primary metabolites |
| url | https://doi.org/10.1186/s12870-025-07082-9 |
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