Comparative study of different centrifugation protocols for a density gradient separation media in isolation of osteoprogenitors from bone marrow aspirate

Comparative study of different centrifugation protocols for a density gradient separation media in isolation of osteoprogenitors from bone marrow aspirate

Introduction: Human bone marrow contains osteoprogenitors capable of differentiating into osteoblasts. Density gradient centrifugation (DGC) is a commonly used method to isolate osteoprogenitors from bone marrow. Numerous studies used different dilution and centrifugation protocols, which might affe...

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Bibliographic Details
Main Authors: Noel Ye Naung, Srisurang Suttapreyasri, Suttatip Kamolmatyakul, Thongchai Nuntanaranont
Format: Article
Language:English
Published: Elsevier 2014-09-01
Series:Journal of Oral Biology and Craniofacial Research
Subjects:
Bone marrow stromal cells
Osteoprogenitor cells
Density gradient centrifugation
Ficoll
STRO-1
Online Access:http://www.sciencedirect.com/science/article/pii/S221242681400061X
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