Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice
Serine Incorporator 2(SERINC2), a highly conserved eukaryotic membrane lipid regulator, promotes critical membrane lipid biosynthesis to maintain neuronal metabolic homeostasis by assembling serine synthase complexes. Our team previously identified SERINC2 as a bipolar disorder (BD) risk gene in Chi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
|
| Series: | Brain Research Bulletin |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923025003314 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849225331854016512 |
|---|---|
| author | Tianlang Ke Ruilan Yang Zewen Tan Liping Cao Xuemei Liu |
| author_facet | Tianlang Ke Ruilan Yang Zewen Tan Liping Cao Xuemei Liu |
| author_sort | Tianlang Ke |
| collection | DOAJ |
| description | Serine Incorporator 2(SERINC2), a highly conserved eukaryotic membrane lipid regulator, promotes critical membrane lipid biosynthesis to maintain neuronal metabolic homeostasis by assembling serine synthase complexes. Our team previously identified SERINC2 as a bipolar disorder (BD) risk gene in Chinese Han familial BD cases, with mutations affecting cerebral white matter volume. Despite established clinical relevance, brain expression patterns of SERINC2 remains poorly characterized experimentally. We used RNAscope® in situ hybridization to comprehensively map SERINC2 mRNA distribution across mouse coronal brain sections, and quantified positive cells across regions. Given BD's potential association with excitatory/inhibitory imbalance, multiplex fluorescence in situ hybridization examined SERINC2 co-expression patterns with vesicular glutamate transporter 2 (VGLUT2, marker for glutamatergic neurons) and glutamic acid decarboxylase 2 (GAD2, marker for GABAergic neurons) in SERINC2-positive regions. Widespread SERINC2 expression was observed in in the telencephalon and diencephalon, including cortical areas (neocortex and limbic cortex), hippocampal subregions (CA1 - CA3, dentate gyrus, and subiculum), thalamic nuclei, and basolateral amygdala. High-intensity signals were detected in hindbrain regions, particularly the pontine nuclei (PN) and reticulotegmental nucleus of the pons (RtTg). Cerebellar Purkinje and granular layers also exhibited significant expression. Notably, multiplex fluorescence in situ hybridization revealed SERINC2 + /VGLUT2 + co-expression in cortical layers III-IV, dorsal subiculum, thalamus, PN, and RtTg. Co-expression of SERINC2 + /GAD2 + was identified in the cerebellar Purkinje cell layer. These findings delineate the brain-wide expression landscape of SERINC2 in mice and provide a neuroanatomical basis for studying its role in excitation/inhibition imbalance in the pathogenesis of BD. |
| format | Article |
| id | doaj-art-6a14029589be4bb4bf16d98e8e1e239d |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-6a14029589be4bb4bf16d98e8e1e239d2025-08-25T04:13:58ZengElsevierBrain Research Bulletin1873-27472025-10-0123011151910.1016/j.brainresbull.2025.111519Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J miceTianlang Ke0Ruilan Yang1Zewen Tan2Liping Cao3Xuemei Liu4The Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou Medical University, Guangzhou, ChinaThe Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou Medical University, Guangzhou, ChinaThe Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou Medical University, Guangzhou, ChinaThe Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou Medical University, Guangzhou, China; Corresponding author at: The Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, China.CAS Key Laboratory of Brain Connectome and Manipulation, Shenzhen–Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; University of the Chinese Academy of Sciences, Beijing 100049, China; Shenzhen Key Lab of Neuropsychiatric Modulation, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China; Key Laboratory of Brain Cognition and Brain-inspired Intelligence Technology, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China; Corresponding author at: CAS Key Laboratory of Brain Connectome and Manipulation, Shenzhen–Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.Serine Incorporator 2(SERINC2), a highly conserved eukaryotic membrane lipid regulator, promotes critical membrane lipid biosynthesis to maintain neuronal metabolic homeostasis by assembling serine synthase complexes. Our team previously identified SERINC2 as a bipolar disorder (BD) risk gene in Chinese Han familial BD cases, with mutations affecting cerebral white matter volume. Despite established clinical relevance, brain expression patterns of SERINC2 remains poorly characterized experimentally. We used RNAscope® in situ hybridization to comprehensively map SERINC2 mRNA distribution across mouse coronal brain sections, and quantified positive cells across regions. Given BD's potential association with excitatory/inhibitory imbalance, multiplex fluorescence in situ hybridization examined SERINC2 co-expression patterns with vesicular glutamate transporter 2 (VGLUT2, marker for glutamatergic neurons) and glutamic acid decarboxylase 2 (GAD2, marker for GABAergic neurons) in SERINC2-positive regions. Widespread SERINC2 expression was observed in in the telencephalon and diencephalon, including cortical areas (neocortex and limbic cortex), hippocampal subregions (CA1 - CA3, dentate gyrus, and subiculum), thalamic nuclei, and basolateral amygdala. High-intensity signals were detected in hindbrain regions, particularly the pontine nuclei (PN) and reticulotegmental nucleus of the pons (RtTg). Cerebellar Purkinje and granular layers also exhibited significant expression. Notably, multiplex fluorescence in situ hybridization revealed SERINC2 + /VGLUT2 + co-expression in cortical layers III-IV, dorsal subiculum, thalamus, PN, and RtTg. Co-expression of SERINC2 + /GAD2 + was identified in the cerebellar Purkinje cell layer. These findings delineate the brain-wide expression landscape of SERINC2 in mice and provide a neuroanatomical basis for studying its role in excitation/inhibition imbalance in the pathogenesis of BD.http://www.sciencedirect.com/science/article/pii/S0361923025003314Serine incorporator 2Bipolar disorderRNAscope |
| spellingShingle | Tianlang Ke Ruilan Yang Zewen Tan Liping Cao Xuemei Liu Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice Brain Research Bulletin Serine incorporator 2 Bipolar disorder RNAscope |
| title | Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice |
| title_full | Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice |
| title_fullStr | Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice |
| title_full_unstemmed | Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice |
| title_short | Whole-brain spatial distribution of serine incorporator 2 mRNA in adult C57BL/6 J mice |
| title_sort | whole brain spatial distribution of serine incorporator 2 mrna in adult c57bl 6 j mice |
| topic | Serine incorporator 2 Bipolar disorder RNAscope |
| url | http://www.sciencedirect.com/science/article/pii/S0361923025003314 |
| work_keys_str_mv | AT tianlangke wholebrainspatialdistributionofserineincorporator2mrnainadultc57bl6jmice AT ruilanyang wholebrainspatialdistributionofserineincorporator2mrnainadultc57bl6jmice AT zewentan wholebrainspatialdistributionofserineincorporator2mrnainadultc57bl6jmice AT lipingcao wholebrainspatialdistributionofserineincorporator2mrnainadultc57bl6jmice AT xuemeiliu wholebrainspatialdistributionofserineincorporator2mrnainadultc57bl6jmice |