Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway
Objectives: This study aimed to evaluate the effects of Biodentine on human Dental Pulp Stem Cells (hDPSCs) proliferation and senescence and explore the underlying anti-aging mechanism. Methods: hDPSCs were isolated from two groups of patients aged 18 to 27 and 60 to 70 years and treated with Bioden...
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| Language: | English |
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Elsevier
2025-08-01
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| Series: | International Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0020653925001078 |
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| author | Qi Zhang Shujie Cao Min Zhang Daming Wu Chengfei Zhang Jin Li |
| author_facet | Qi Zhang Shujie Cao Min Zhang Daming Wu Chengfei Zhang Jin Li |
| author_sort | Qi Zhang |
| collection | DOAJ |
| description | Objectives: This study aimed to evaluate the effects of Biodentine on human Dental Pulp Stem Cells (hDPSCs) proliferation and senescence and explore the underlying anti-aging mechanism. Methods: hDPSCs were isolated from two groups of patients aged 18 to 27 and 60 to 70 years and treated with Biodentine extract. The optimal concentration of Biodentine extract was determined using the CCK-8 assay. Biodentine treatment on hDPSCs proliferation and aging was assessed using qRT-PCR and Western blot. The small molecule inhibitor XAV939, targeting the Wnt/β-catenin signaling pathway, was added to investigate the anti-aging mechanism of Biodentine in hDPSCs. Results: The isolated hDPSCs exhibited mesenchymal stem cells (MSCs) characteristics, and the proportion of S-phased cells decreased significantly in the aged group. Biodentine promoted hDPSCs proliferation, with the most pronounced effect observed at 0.2 mg/mL. qRT-PCR showed that Biodentine enhanced the expression of odonto/osteogenesis-related genes. Senescence-associated β-galactosidase staining and Western blot analysis further demonstrated the significant anti-aging effect of Biodentine on hDPSCs. Western blot revealed that Biodentine exerted its anti-aging effect through the Wnt/β-catenin signaling pathway. Conclusion: The study demonstrated that Biodentine promotes proliferation and exerts anti-aging effects on hDPSCs through the Wnt/β-catenin pathway. |
| format | Article |
| id | doaj-art-6a0cfe88b3d04c7c8a90fde1d36992ed |
| institution | OA Journals |
| issn | 0020-6539 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | International Dental Journal |
| spelling | doaj-art-6a0cfe88b3d04c7c8a90fde1d36992ed2025-08-20T02:37:20ZengElsevierInternational Dental Journal0020-65392025-08-0175410081910.1016/j.identj.2025.03.028Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin PathwayQi Zhang0Shujie Cao1Min Zhang2Daming Wu3Chengfei Zhang4Jin Li5Department of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaRestorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaDepartment of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China; Corresponding author. Department of Geriatric Dentistry, The Affiliated Stomatological Hospital of Nanjing Medical University, 136 Hanzhong Road, Nanjing 210029, China.Objectives: This study aimed to evaluate the effects of Biodentine on human Dental Pulp Stem Cells (hDPSCs) proliferation and senescence and explore the underlying anti-aging mechanism. Methods: hDPSCs were isolated from two groups of patients aged 18 to 27 and 60 to 70 years and treated with Biodentine extract. The optimal concentration of Biodentine extract was determined using the CCK-8 assay. Biodentine treatment on hDPSCs proliferation and aging was assessed using qRT-PCR and Western blot. The small molecule inhibitor XAV939, targeting the Wnt/β-catenin signaling pathway, was added to investigate the anti-aging mechanism of Biodentine in hDPSCs. Results: The isolated hDPSCs exhibited mesenchymal stem cells (MSCs) characteristics, and the proportion of S-phased cells decreased significantly in the aged group. Biodentine promoted hDPSCs proliferation, with the most pronounced effect observed at 0.2 mg/mL. qRT-PCR showed that Biodentine enhanced the expression of odonto/osteogenesis-related genes. Senescence-associated β-galactosidase staining and Western blot analysis further demonstrated the significant anti-aging effect of Biodentine on hDPSCs. Western blot revealed that Biodentine exerted its anti-aging effect through the Wnt/β-catenin signaling pathway. Conclusion: The study demonstrated that Biodentine promotes proliferation and exerts anti-aging effects on hDPSCs through the Wnt/β-catenin pathway.http://www.sciencedirect.com/science/article/pii/S0020653925001078BiodentinehDPSCsVital Pulp TherapyAnti-AgingXAV939 |
| spellingShingle | Qi Zhang Shujie Cao Min Zhang Daming Wu Chengfei Zhang Jin Li Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway International Dental Journal Biodentine hDPSCs Vital Pulp Therapy Anti-Aging XAV939 |
| title | Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway |
| title_full | Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway |
| title_fullStr | Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway |
| title_full_unstemmed | Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway |
| title_short | Biodentine Counteracts the Aging Process of Human Dental Pulp Stem Cells Through Wnt/β-Catenin Pathway |
| title_sort | biodentine counteracts the aging process of human dental pulp stem cells through wnt β catenin pathway |
| topic | Biodentine hDPSCs Vital Pulp Therapy Anti-Aging XAV939 |
| url | http://www.sciencedirect.com/science/article/pii/S0020653925001078 |
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