Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties
Magnesium chelating is considered a promising method to increase Mg uptake, thus fight against the prevalent case of Mg deficiency. In this study, hydrolysates and peptide fractions from spent brewer’s yeast (SBY) and soybean meal (SBM) were evaluated for the magnesium chelating ability. Despite the...
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Elsevier
2025-09-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X25000721 |
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| author | Bao Ninh Tran Thi Bich Ngoc Ha Phuong Mai Bui Hang Thuy Dam Kim Anh To Tuan Anh Pham Tuan Le |
| author_facet | Bao Ninh Tran Thi Bich Ngoc Ha Phuong Mai Bui Hang Thuy Dam Kim Anh To Tuan Anh Pham Tuan Le |
| author_sort | Bao Ninh Tran |
| collection | DOAJ |
| description | Magnesium chelating is considered a promising method to increase Mg uptake, thus fight against the prevalent case of Mg deficiency. In this study, hydrolysates and peptide fractions from spent brewer’s yeast (SBY) and soybean meal (SBM) were evaluated for the magnesium chelating ability. Despite the similar amino acid profile and protein concentration, SBY hydrolysate showed superior chelating yield than that of SBM. Cross flow filtration was shown to have facilitated the chelating process, with the specific chelating yield peaked at 94.44 mg/g protein for the 3 kDa peptide fraction of SBY hydrolysate. It was also demonstrated that at optimum conditions (pH 2 to 4 and Mg loaded 5 mM to 25 mM), the ≤ 3 kDa peptide fraction of SBY exhibited the highest magnesium chelating yield of more than 98 %. Physical and biochemistry properties of spray dried peptide chelate were also scrutinized. The FTIR spectra confirmed the chelating sites of peptide were amino and carboxyl groups; and the particle size distribution of Mg chelated peptides was identified in the range of 1000 nm. Our findings concerning the in-vitro gastrointestinal stability and cytotoxicity have confirmed the suitability of magnesium chelated SBY for animal and human consumption. |
| format | Article |
| id | doaj-art-6a0aee62f44c4301a0dbcbb0778b93fc |
| institution | Kabale University |
| issn | 1687-157X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj-art-6a0aee62f44c4301a0dbcbb0778b93fc2025-08-24T05:11:48ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2025-09-0123310052810.1016/j.jgeb.2025.100528Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and propertiesBao Ninh Tran0Thi Bich Ngoc Ha1Phuong Mai Bui2Hang Thuy Dam3Kim Anh To4Tuan Anh Pham5Tuan Le6School of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamSchool of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamSchool of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamSchool of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamSchool of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamSchool of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamCorresponding author.; School of Chemistry and Life Sciences (SCLS), Hanoi University of Science and Technology (HUST), Hanoi, Viet NamMagnesium chelating is considered a promising method to increase Mg uptake, thus fight against the prevalent case of Mg deficiency. In this study, hydrolysates and peptide fractions from spent brewer’s yeast (SBY) and soybean meal (SBM) were evaluated for the magnesium chelating ability. Despite the similar amino acid profile and protein concentration, SBY hydrolysate showed superior chelating yield than that of SBM. Cross flow filtration was shown to have facilitated the chelating process, with the specific chelating yield peaked at 94.44 mg/g protein for the 3 kDa peptide fraction of SBY hydrolysate. It was also demonstrated that at optimum conditions (pH 2 to 4 and Mg loaded 5 mM to 25 mM), the ≤ 3 kDa peptide fraction of SBY exhibited the highest magnesium chelating yield of more than 98 %. Physical and biochemistry properties of spray dried peptide chelate were also scrutinized. The FTIR spectra confirmed the chelating sites of peptide were amino and carboxyl groups; and the particle size distribution of Mg chelated peptides was identified in the range of 1000 nm. Our findings concerning the in-vitro gastrointestinal stability and cytotoxicity have confirmed the suitability of magnesium chelated SBY for animal and human consumption.http://www.sciencedirect.com/science/article/pii/S1687157X25000721ChelatePeptideSpent brewer’s yeastSoybean mealMagnesium |
| spellingShingle | Bao Ninh Tran Thi Bich Ngoc Ha Phuong Mai Bui Hang Thuy Dam Kim Anh To Tuan Anh Pham Tuan Le Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties Journal of Genetic Engineering and Biotechnology Chelate Peptide Spent brewer’s yeast Soybean meal Magnesium |
| title | Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties |
| title_full | Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties |
| title_fullStr | Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties |
| title_full_unstemmed | Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties |
| title_short | Magnesium chelation of low molecular weight peptides from protein-rich industrial wastes: Production and properties |
| title_sort | magnesium chelation of low molecular weight peptides from protein rich industrial wastes production and properties |
| topic | Chelate Peptide Spent brewer’s yeast Soybean meal Magnesium |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X25000721 |
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