Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics
The bacterial diversity and metabolomic characteristics of yogurt from three different producers in Bole, Xinjiang were analyzed by using Illumina sequencing and untargeted metabolomics, and the relationship between them was explored. Bacterial diversity analysis showed that the major bacterial gene...
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China Food Publishing Company
2025-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-014.pdf |
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| author | WANG Qi, ZENG Jun, HUO Xiangdong, XUE Juanjuan, LOU Kai, LIN Qing, ZHAO Zhongkai, GAO Yan |
| author_facet | WANG Qi, ZENG Jun, HUO Xiangdong, XUE Juanjuan, LOU Kai, LIN Qing, ZHAO Zhongkai, GAO Yan |
| author_sort | WANG Qi, ZENG Jun, HUO Xiangdong, XUE Juanjuan, LOU Kai, LIN Qing, ZHAO Zhongkai, GAO Yan |
| collection | DOAJ |
| description | The bacterial diversity and metabolomic characteristics of yogurt from three different producers in Bole, Xinjiang were analyzed by using Illumina sequencing and untargeted metabolomics, and the relationship between them was explored. Bacterial diversity analysis showed that the major bacterial genera in the yogurt samples were Lactobacillus, Klebsiella, Lactococcus, and Acetobacter. Using linear discriminant analysis effect size (LEfSe) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt), Lactobacillus, Klebsiella, and Lactococcus were identified as biomarkers, and their functions were predicted. The metabolomic analysis identified a total of 397 metabolites in the positive ion mode and 324 metabolites in the negative ion mode, with lipids and lipid-like substances, organic acids and their derivatives, and organic heterocyclic compounds being the predominant ones. Notably, lipids and lipid-like substances, organic acids and their derivatives were the major differential metabolites, contributing to the flavor of yogurt. The metabolic pathway analysis revealed amino acid metabolism and secondary metabolism to be the main enriched pathways, which aligned partially with the functional prediction results. Integrated microbiomic and metabolomic analyses showed that the genera Lactobacillus, Limosilactobacillus, Lactococcus, and Klebsiella were significantly correlated with some metabolites such as organic acids and their derivatives, as well as lipids and lipid-like substances, indicating that the microbial community affected the nutrients of yogurt through its metabolic activities. This study provides a scientific theoretical basis for the production and quality improvement of Xinjiang yogurt. |
| format | Article |
| id | doaj-art-69fd91a729784fbe8e9c5d5ae35b8c4d |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-69fd91a729784fbe8e9c5d5ae35b8c4d2025-08-20T03:25:07ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461112513810.7506/spkx1002-6630-20241119-140Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted MetabolomicsWANG Qi, ZENG Jun, HUO Xiangdong, XUE Juanjuan, LOU Kai, LIN Qing, ZHAO Zhongkai, GAO Yan0(1. College of Smart Agriculture (Research Institute), Xinjiang University, ürümqi 830017, China; 2. Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, ürümqi 830091, China)The bacterial diversity and metabolomic characteristics of yogurt from three different producers in Bole, Xinjiang were analyzed by using Illumina sequencing and untargeted metabolomics, and the relationship between them was explored. Bacterial diversity analysis showed that the major bacterial genera in the yogurt samples were Lactobacillus, Klebsiella, Lactococcus, and Acetobacter. Using linear discriminant analysis effect size (LEfSe) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt), Lactobacillus, Klebsiella, and Lactococcus were identified as biomarkers, and their functions were predicted. The metabolomic analysis identified a total of 397 metabolites in the positive ion mode and 324 metabolites in the negative ion mode, with lipids and lipid-like substances, organic acids and their derivatives, and organic heterocyclic compounds being the predominant ones. Notably, lipids and lipid-like substances, organic acids and their derivatives were the major differential metabolites, contributing to the flavor of yogurt. The metabolic pathway analysis revealed amino acid metabolism and secondary metabolism to be the main enriched pathways, which aligned partially with the functional prediction results. Integrated microbiomic and metabolomic analyses showed that the genera Lactobacillus, Limosilactobacillus, Lactococcus, and Klebsiella were significantly correlated with some metabolites such as organic acids and their derivatives, as well as lipids and lipid-like substances, indicating that the microbial community affected the nutrients of yogurt through its metabolic activities. This study provides a scientific theoretical basis for the production and quality improvement of Xinjiang yogurt.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-014.pdfyogurt; bacterial diversity; untargeted metabolomics; illumina sequencing; xinjiang’s bole |
| spellingShingle | WANG Qi, ZENG Jun, HUO Xiangdong, XUE Juanjuan, LOU Kai, LIN Qing, ZHAO Zhongkai, GAO Yan Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics Shipin Kexue yogurt; bacterial diversity; untargeted metabolomics; illumina sequencing; xinjiang’s bole |
| title | Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics |
| title_full | Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics |
| title_fullStr | Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics |
| title_full_unstemmed | Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics |
| title_short | Yogurts from Different Producers in Bole, Xinjiang: Bacterial Diversity and Community Structure and Differences in Metabolite Profile as Revealed by Untargeted Metabolomics |
| title_sort | yogurts from different producers in bole xinjiang bacterial diversity and community structure and differences in metabolite profile as revealed by untargeted metabolomics |
| topic | yogurt; bacterial diversity; untargeted metabolomics; illumina sequencing; xinjiang’s bole |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-11-014.pdf |
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