Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China
In order to solve the problem of low and poor straw degradation in typical black soil areas of Northeast China, the present study was carried out to screen the potential of in situ strains with cellulose degradation ability from black soils of Northeast China to play a role in the resourceful utiliz...
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MDPI AG
2024-11-01
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| Series: | Agronomy |
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| author | Lei Zhang Tianyu Liu Chengzhao Yan Jinpeng Zhang Rui Yu Nana Luo Yong Yu |
| author_facet | Lei Zhang Tianyu Liu Chengzhao Yan Jinpeng Zhang Rui Yu Nana Luo Yong Yu |
| author_sort | Lei Zhang |
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| description | In order to solve the problem of low and poor straw degradation in typical black soil areas of Northeast China, the present study was carried out to screen the potential of in situ strains with cellulose degradation ability from black soils of Northeast China to play a role in the resourceful utilization of straw and the development of sustainable agriculture. The straw degradation potential of the strains was evaluated by combining sodium carboxymethyl cellulose plate screening and cellulase viability assay; the species identification of the strains was carried out by morphology, physiology, biochemistry, and molecular biology; and the basic medium formulation of the strains was optimized by Box–Behnken response surface methodology. Ten cellulose-degrading strains were identified: ZL-5, ZL-69, ZL-88, ZL-95, ZL-111, ZL-137, ZL-139, ZL-140, ZL-187, and ZL-216, of which ZL-139 had the highest cellulase production capacity, with a cellulase secretion of 7.8781 U/mL in the enzyme-producing medium. ZL-139 was identified as <i>Bacillus cereus</i>; the optimized best formulation was glucose—4.284 g/L, yeast extract—1.454 g/L, MgSO<sub>4</sub>—0.417 g/L, KH<sub>2</sub>PO<sub>4</sub>—0.5 g/L, KH<sub>2</sub>PO<sub>4</sub>—0.5 g/L, K<sub>2</sub>HPO<sub>4</sub>—1.5 g/L, and NaCl—1.0 g/L. In conclusion, strain ZL-139 has good potential for crop straw degradation and can be a candidate strain for a straw-rotting agent in northeast China, with promising prospects for development and utilization. |
| format | Article |
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| institution | OA Journals |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Agronomy |
| spelling | doaj-art-69fcddd3a6f4405792a2bd64ecd4ed6b2025-08-20T02:07:56ZengMDPI AGAgronomy2073-43952024-11-011411259110.3390/agronomy14112591Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of ChinaLei Zhang0Tianyu Liu1Chengzhao Yan2Jinpeng Zhang3Rui Yu4Nana Luo5Yong Yu6Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Resources and Environment, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaKey Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaKey Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaKey Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaIn order to solve the problem of low and poor straw degradation in typical black soil areas of Northeast China, the present study was carried out to screen the potential of in situ strains with cellulose degradation ability from black soils of Northeast China to play a role in the resourceful utilization of straw and the development of sustainable agriculture. The straw degradation potential of the strains was evaluated by combining sodium carboxymethyl cellulose plate screening and cellulase viability assay; the species identification of the strains was carried out by morphology, physiology, biochemistry, and molecular biology; and the basic medium formulation of the strains was optimized by Box–Behnken response surface methodology. Ten cellulose-degrading strains were identified: ZL-5, ZL-69, ZL-88, ZL-95, ZL-111, ZL-137, ZL-139, ZL-140, ZL-187, and ZL-216, of which ZL-139 had the highest cellulase production capacity, with a cellulase secretion of 7.8781 U/mL in the enzyme-producing medium. ZL-139 was identified as <i>Bacillus cereus</i>; the optimized best formulation was glucose—4.284 g/L, yeast extract—1.454 g/L, MgSO<sub>4</sub>—0.417 g/L, KH<sub>2</sub>PO<sub>4</sub>—0.5 g/L, KH<sub>2</sub>PO<sub>4</sub>—0.5 g/L, K<sub>2</sub>HPO<sub>4</sub>—1.5 g/L, and NaCl—1.0 g/L. In conclusion, strain ZL-139 has good potential for crop straw degradation and can be a candidate strain for a straw-rotting agent in northeast China, with promising prospects for development and utilization.https://www.mdpi.com/2073-4395/14/11/2591black soilstraw resource utilizationcellulase<i>Bacillus cereus</i>Box–Behnken response surface methodology |
| spellingShingle | Lei Zhang Tianyu Liu Chengzhao Yan Jinpeng Zhang Rui Yu Nana Luo Yong Yu Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China Agronomy black soil straw resource utilization cellulase <i>Bacillus cereus</i> Box–Behnken response surface methodology |
| title | Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China |
| title_full | Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China |
| title_fullStr | Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China |
| title_full_unstemmed | Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China |
| title_short | Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China |
| title_sort | isolation characterization and optimization of culture medium for local straw degrading bacteria from northeastern black soils of china |
| topic | black soil straw resource utilization cellulase <i>Bacillus cereus</i> Box–Behnken response surface methodology |
| url | https://www.mdpi.com/2073-4395/14/11/2591 |
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