The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells
Abstract Tartrazine (E102) is a controversial coloring agent whose potential impacts on human health are not fully understood. Our study reveals the vascular disrupting effects of tartrazine (TTZ) on developing zebrafish embryos in vivo and on human umbilical vein endothelial cells in vitro. The dye...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82076-5 |
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| author | Dinh Duy Thanh Nguyen Bich-Ngoc Cécile Paques Aurélie Christian Stéphanie Herkenne Ingrid Struman Marc Muller |
| author_facet | Dinh Duy Thanh Nguyen Bich-Ngoc Cécile Paques Aurélie Christian Stéphanie Herkenne Ingrid Struman Marc Muller |
| author_sort | Dinh Duy Thanh |
| collection | DOAJ |
| description | Abstract Tartrazine (E102) is a controversial coloring agent whose potential impacts on human health are not fully understood. Our study reveals the vascular disrupting effects of tartrazine (TTZ) on developing zebrafish embryos in vivo and on human umbilical vein endothelial cells in vitro. The dye was shown to cause dose-dependent hemorrhages in zebrafish embryos. Analyzing transgenic zebrafish harboring fluorescent endothelial cells revealed that TTZ treatment disrupted cell organization into vessels in both the sub-intestinal vein and the brain area. Assays on human umbilical vein endothelial cells demonstrated that TTZ inhibited endothelial proliferation, tube formation, and migration in a dose-dependent manner. Taken together, our results indicate for the first time that TTZ can affect endothelial cell properties, possibly by disrupting Rho family GTPase pathways which control the cytoskeleton. Our finding provides a credible explanation for many reported human health impacts and offers prospective applications for biomedicine. |
| format | Article |
| id | doaj-art-69f79af4148e41ebb877a60f86b3391c |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-69f79af4148e41ebb877a60f86b3391c2025-08-20T02:20:38ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-82076-5The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cellsDinh Duy Thanh0Nguyen Bich-Ngoc1Cécile Paques2Aurélie Christian3Stéphanie Herkenne4Ingrid Struman5Marc Muller6Lab. for Organogenesis and Regeneration, GIGA-Institute, Université de LiègeVNU School of Interdisciplinary Sciences and Arts, Vietnam National UniversityLab. of Molecular Angiogenesis, GIGA-Institute, Université de LiègeLab. of Molecular Angiogenesis, GIGA-Institute, Université de LiègeLab. of Molecular Angiogenesis, GIGA-Institute, Université de LiègeLab. of Molecular Angiogenesis, GIGA-Institute, Université de LiègeLab. for Organogenesis and Regeneration, GIGA-Institute, Université de LiègeAbstract Tartrazine (E102) is a controversial coloring agent whose potential impacts on human health are not fully understood. Our study reveals the vascular disrupting effects of tartrazine (TTZ) on developing zebrafish embryos in vivo and on human umbilical vein endothelial cells in vitro. The dye was shown to cause dose-dependent hemorrhages in zebrafish embryos. Analyzing transgenic zebrafish harboring fluorescent endothelial cells revealed that TTZ treatment disrupted cell organization into vessels in both the sub-intestinal vein and the brain area. Assays on human umbilical vein endothelial cells demonstrated that TTZ inhibited endothelial proliferation, tube formation, and migration in a dose-dependent manner. Taken together, our results indicate for the first time that TTZ can affect endothelial cell properties, possibly by disrupting Rho family GTPase pathways which control the cytoskeleton. Our finding provides a credible explanation for many reported human health impacts and offers prospective applications for biomedicine.https://doi.org/10.1038/s41598-024-82076-5TartrazineBlood vessels, ZebrafishHUVECBMP pathwaysRho GTPases |
| spellingShingle | Dinh Duy Thanh Nguyen Bich-Ngoc Cécile Paques Aurélie Christian Stéphanie Herkenne Ingrid Struman Marc Muller The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells Scientific Reports Tartrazine Blood vessels, Zebrafish HUVEC BMP pathways Rho GTPases |
| title | The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| title_full | The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| title_fullStr | The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| title_full_unstemmed | The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| title_short | The food dye Tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| title_sort | food dye tartrazine disrupts vascular formation both in zebrafish larvae and in human primary endothelial cells |
| topic | Tartrazine Blood vessels, Zebrafish HUVEC BMP pathways Rho GTPases |
| url | https://doi.org/10.1038/s41598-024-82076-5 |
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