Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection
Abstract Viruses exploit cellular machinery to complete their replication cycle. Furthering our understanding of this process provides insight into the mechanism of virus replication and potential targets for antiviral therapeutics. Genome-wide CRISPR screens have identified cellular pathways import...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05669-3 |
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| author | Christina L. Rootes Karla J. Cowley Aaron M. Brice Henry G. Beetham Rasan Mohamed Sathiqu Kaylene J. Simpson Cameron R. Stewart |
| author_facet | Christina L. Rootes Karla J. Cowley Aaron M. Brice Henry G. Beetham Rasan Mohamed Sathiqu Kaylene J. Simpson Cameron R. Stewart |
| author_sort | Christina L. Rootes |
| collection | DOAJ |
| description | Abstract Viruses exploit cellular machinery to complete their replication cycle. Furthering our understanding of this process provides insight into the mechanism of virus replication and potential targets for antiviral therapeutics. Genome-wide CRISPR screens have identified cellular pathways important in the SARS-COV-2 infection process, including vesicular traffic, lipid homeostasis and PI3K signalling. Functional genomics-driven analysis of host-encoded microRNAs (miRNAs) impacting SARS-CoV-2 infection would provide further unbiased and discovery-driven insight into the host-pathogen interface. Here we present findings from genome-wide complementary miRNA mimic and inhibitor screens performed in a bio-safety level (BSL)-4 laboratory using a combination of high-throughput robotics, high-content imaging and novel data analysis pipelines. This dataset has identified both miRNA promoters and inhibitors of SARS-CoV-2 replication which may be used by researchers to further explore therapeutic targets against SARS-CoV-2 and the host factors influencing COVID pathogenesis. |
| format | Article |
| id | doaj-art-69f0d954cb2e4cc28aeb89b53619dd7c |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-69f0d954cb2e4cc28aeb89b53619dd7c2025-08-20T04:01:47ZengNature PortfolioScientific Data2052-44632025-07-0112111310.1038/s41597-025-05669-3Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infectionChristina L. Rootes0Karla J. Cowley1Aaron M. Brice2Henry G. Beetham3Rasan Mohamed Sathiqu4Kaylene J. Simpson5Cameron R. Stewart6CSIRO Health & Biosecurity, Australian Centre for Disease PreparednessVictorian Centre for Functional Genomics, Peter MacCallum Cancer CentreCSIRO Health & Biosecurity, Australian Centre for Disease PreparednessVictorian Centre for Functional Genomics, Peter MacCallum Cancer CentreCSIRO Health & Biosecurity, Australian Centre for Disease PreparednessVictorian Centre for Functional Genomics, Peter MacCallum Cancer CentreCSIRO Health & Biosecurity, Australian Centre for Disease PreparednessAbstract Viruses exploit cellular machinery to complete their replication cycle. Furthering our understanding of this process provides insight into the mechanism of virus replication and potential targets for antiviral therapeutics. Genome-wide CRISPR screens have identified cellular pathways important in the SARS-COV-2 infection process, including vesicular traffic, lipid homeostasis and PI3K signalling. Functional genomics-driven analysis of host-encoded microRNAs (miRNAs) impacting SARS-CoV-2 infection would provide further unbiased and discovery-driven insight into the host-pathogen interface. Here we present findings from genome-wide complementary miRNA mimic and inhibitor screens performed in a bio-safety level (BSL)-4 laboratory using a combination of high-throughput robotics, high-content imaging and novel data analysis pipelines. This dataset has identified both miRNA promoters and inhibitors of SARS-CoV-2 replication which may be used by researchers to further explore therapeutic targets against SARS-CoV-2 and the host factors influencing COVID pathogenesis.https://doi.org/10.1038/s41597-025-05669-3 |
| spellingShingle | Christina L. Rootes Karla J. Cowley Aaron M. Brice Henry G. Beetham Rasan Mohamed Sathiqu Kaylene J. Simpson Cameron R. Stewart Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection Scientific Data |
| title | Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection |
| title_full | Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection |
| title_fullStr | Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection |
| title_full_unstemmed | Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection |
| title_short | Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection |
| title_sort | genome wide analysis of host encoded micrornas modulating sars cov 2 infection |
| url | https://doi.org/10.1038/s41597-025-05669-3 |
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