Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn.
Under the current context of intensive farming, small-horned animals are more suitable for large-scale breeding. The CASP14 gene, closely associated with skin and keratin formation, may influence horn size due to its link with skin development. This study comprehensively analyzed the tissue-specific...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0310464 |
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| author | Xiaoning Lu Guoqing Zhang Hao Yang Mingzhu Shan Xiaoxu Zhang Yuqin Wang Junyan Bai Zhangyuan Pan |
| author_facet | Xiaoning Lu Guoqing Zhang Hao Yang Mingzhu Shan Xiaoxu Zhang Yuqin Wang Junyan Bai Zhangyuan Pan |
| author_sort | Xiaoning Lu |
| collection | DOAJ |
| description | Under the current context of intensive farming, small-horned animals are more suitable for large-scale breeding. The CASP14 gene, closely associated with skin and keratin formation, may influence horn size due to its link with skin development. This study comprehensively analyzed the tissue-specific expression of CASP14 using RNA-Seq data, identified functional sites through whole-genome sequencing (WGS), and investigated allele-specific expression (ASE) validated by KASP assays. Results showed significantly higher CASP14 expression in the scurred group com-pared to the SHE group, with pronounced expression in the skin. Interbreed comparisons also revealed elevated CASP14 levels in the scurred group. Analysis of potential functional sites indicated structural similarities in the CASP14 protein among horned mammals. Five ASE events were discovered, and intersecting these with SNPs and high fixation index loci (Fst > 0.05) identified three potential functional sites: 7941628, 7941817, and 7941830. The SNP site 7944295 was selected for T-test analysis and further validated through KASP assays, corroborating the role of CASP14 in sheep horn phenotypes. Our findings suggest that CASP14 plays a significant role in horn development, offering insights into breeding strategies for small-horned animals. |
| format | Article |
| id | doaj-art-69e1d9750c3f4d6f8d4e701cb678e421 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-69e1d9750c3f4d6f8d4e701cb678e4212025-08-20T02:23:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011911e031046410.1371/journal.pone.0310464Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn.Xiaoning LuGuoqing ZhangHao YangMingzhu ShanXiaoxu ZhangYuqin WangJunyan BaiZhangyuan PanUnder the current context of intensive farming, small-horned animals are more suitable for large-scale breeding. The CASP14 gene, closely associated with skin and keratin formation, may influence horn size due to its link with skin development. This study comprehensively analyzed the tissue-specific expression of CASP14 using RNA-Seq data, identified functional sites through whole-genome sequencing (WGS), and investigated allele-specific expression (ASE) validated by KASP assays. Results showed significantly higher CASP14 expression in the scurred group com-pared to the SHE group, with pronounced expression in the skin. Interbreed comparisons also revealed elevated CASP14 levels in the scurred group. Analysis of potential functional sites indicated structural similarities in the CASP14 protein among horned mammals. Five ASE events were discovered, and intersecting these with SNPs and high fixation index loci (Fst > 0.05) identified three potential functional sites: 7941628, 7941817, and 7941830. The SNP site 7944295 was selected for T-test analysis and further validated through KASP assays, corroborating the role of CASP14 in sheep horn phenotypes. Our findings suggest that CASP14 plays a significant role in horn development, offering insights into breeding strategies for small-horned animals.https://doi.org/10.1371/journal.pone.0310464 |
| spellingShingle | Xiaoning Lu Guoqing Zhang Hao Yang Mingzhu Shan Xiaoxu Zhang Yuqin Wang Junyan Bai Zhangyuan Pan Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. PLoS ONE |
| title | Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. |
| title_full | Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. |
| title_fullStr | Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. |
| title_full_unstemmed | Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. |
| title_short | Tissue-specific and functional loci analysis of CASP14 gene in the sheep horn. |
| title_sort | tissue specific and functional loci analysis of casp14 gene in the sheep horn |
| url | https://doi.org/10.1371/journal.pone.0310464 |
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