Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice
Background: The LD50 (lethal dose causing 50% mortality) of ethylene glycol (EG) and its associated toxicity in mice (Mus musculus) were assessed by evaluating kidney function. Aim: This study aimed to determine the acute toxicity of oral lethal dose 50% (LD50) of ethylene glycol, also degenerat...
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Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=224812 |
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| author | Dewa Ketut Meles Imam Mustofa Wurlina Wurlina Clarissa Audreylea Donova Essi Rayareswari Hidayanti Niluh Suwasanti Zulfi Nur Amrina Rosyada Aswin Rafif Khairullah Adeyinka Oye Akintunde Rheza Imawan Mustofa Satriawan Wedniyanto Putra Riza Zainuddin Ahmad Wasito Wasito Ricadonna Raissa |
| author_facet | Dewa Ketut Meles Imam Mustofa Wurlina Wurlina Clarissa Audreylea Donova Essi Rayareswari Hidayanti Niluh Suwasanti Zulfi Nur Amrina Rosyada Aswin Rafif Khairullah Adeyinka Oye Akintunde Rheza Imawan Mustofa Satriawan Wedniyanto Putra Riza Zainuddin Ahmad Wasito Wasito Ricadonna Raissa |
| author_sort | Dewa Ketut Meles |
| collection | DOAJ |
| description | Background:
The LD50 (lethal dose causing 50% mortality) of ethylene glycol (EG) and its associated toxicity in mice (Mus musculus) were assessed by evaluating kidney function.
Aim:
This study aimed to determine the acute toxicity of oral lethal dose 50% (LD50) of ethylene glycol, also degeneration, necrosis, and inflammatory cell invasion in kidney tubules of male rats (Rattus norvegicus) as animal model.
Methods:
There were 66 DDG (Deutschland Denken Yoken) mice in 11 groups of six in this investigation. The daylong metabolic cage study contained one control (C) and ten treatment groups that received different EG doses. EG's daily BW/kg dosage varied from 0.5 mg/kg to 15,000 mg/kg. The treatment group was given a single dose of EG at a dose of T1 = 0.5 mg/kg BW, T2 = 1.57 mg/kg BW, T3 = 4.94 mg/kg BW, T4 = 15.54 mg/kg BW, T5 = 48.84 mg/kg BW, T6 = 153.55 mg/kg BW, T7 = 482.74 mg/kg BW, and T8 = 1517.66 mg/kg BW T9 = 4771.24 mg/kg BW, T10 = 14999.99 ≈ 15. 000 mg/kg BW. One-way ANOVA testing was used to analyze the data.
Results:
The LD50 value of EG in mice was determined to be 1.598 mg/kg BW, classifying EG as "Slightly Toxic." According to renal histopathology, EG dosage increased renal tubular degeneration, necrosis, and interstitial inflammatory cell infiltration. Acute renal impairment and lower urine output were observed in the EG (4.94 mg/kg - 1517.66 mg/kg BW). Histologically, EG levels are associated with renal tubular cell degeneration, necrosis, and interstitial inflammatory cell growth.
Conclusion:
Acute EG exposure caused renal failure in male mice. Acute exposure to EG causes renal tubular cell degeneration and inflammation, indicating toxicity and health hazards. [Open Vet J 2024; 14(12.000): 3539-3551] |
| format | Article |
| id | doaj-art-69d8e484620d43da94fddc95ddde0414 |
| institution | DOAJ |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-69d8e484620d43da94fddc95ddde04142025-08-20T03:09:13ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123539355110.5455/OVJ.2024.v14.i12.36224812Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in miceDewa Ketut Meles0Imam Mustofa1Wurlina Wurlina2Clarissa Audreylea Donova3Essi Rayareswari Hidayanti4Niluh Suwasanti5Zulfi Nur Amrina Rosyada6Aswin Rafif Khairullah7Adeyinka Oye Akintunde8Rheza Imawan Mustofa9Satriawan Wedniyanto Putra10Riza Zainuddin Ahmad11Wasito Wasito12Ricadonna Raissa13Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Profession Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Department of Clinical Pathology, Universitas Katolik Widya Mandala Surabaya, Surabaya, Indonesia Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia Department of Agriculture and Industrial Technology, Babcock University, Ilishan-Remo, Ogun State, Nigeria Physician in Nayaka Hostpital, Surabaya, Indonesia Internship Physician in Rumah Sakit Angkatan Udara, Malang, Indonesia Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, IndonesiaBackground: The LD50 (lethal dose causing 50% mortality) of ethylene glycol (EG) and its associated toxicity in mice (Mus musculus) were assessed by evaluating kidney function. Aim: This study aimed to determine the acute toxicity of oral lethal dose 50% (LD50) of ethylene glycol, also degeneration, necrosis, and inflammatory cell invasion in kidney tubules of male rats (Rattus norvegicus) as animal model. Methods: There were 66 DDG (Deutschland Denken Yoken) mice in 11 groups of six in this investigation. The daylong metabolic cage study contained one control (C) and ten treatment groups that received different EG doses. EG's daily BW/kg dosage varied from 0.5 mg/kg to 15,000 mg/kg. The treatment group was given a single dose of EG at a dose of T1 = 0.5 mg/kg BW, T2 = 1.57 mg/kg BW, T3 = 4.94 mg/kg BW, T4 = 15.54 mg/kg BW, T5 = 48.84 mg/kg BW, T6 = 153.55 mg/kg BW, T7 = 482.74 mg/kg BW, and T8 = 1517.66 mg/kg BW T9 = 4771.24 mg/kg BW, T10 = 14999.99 ≈ 15. 000 mg/kg BW. One-way ANOVA testing was used to analyze the data. Results: The LD50 value of EG in mice was determined to be 1.598 mg/kg BW, classifying EG as "Slightly Toxic." According to renal histopathology, EG dosage increased renal tubular degeneration, necrosis, and interstitial inflammatory cell infiltration. Acute renal impairment and lower urine output were observed in the EG (4.94 mg/kg - 1517.66 mg/kg BW). Histologically, EG levels are associated with renal tubular cell degeneration, necrosis, and interstitial inflammatory cell growth. Conclusion: Acute EG exposure caused renal failure in male mice. Acute exposure to EG causes renal tubular cell degeneration and inflammation, indicating toxicity and health hazards. [Open Vet J 2024; 14(12.000): 3539-3551]http://www.ejmanager.com/fulltextpdf.php?mno=224812diseaseegld50renaltoxic |
| spellingShingle | Dewa Ketut Meles Imam Mustofa Wurlina Wurlina Clarissa Audreylea Donova Essi Rayareswari Hidayanti Niluh Suwasanti Zulfi Nur Amrina Rosyada Aswin Rafif Khairullah Adeyinka Oye Akintunde Rheza Imawan Mustofa Satriawan Wedniyanto Putra Riza Zainuddin Ahmad Wasito Wasito Ricadonna Raissa Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice Open Veterinary Journal disease eg ld50 renal toxic |
| title | Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice |
| title_full | Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice |
| title_fullStr | Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice |
| title_full_unstemmed | Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice |
| title_short | Acute toxicity effects of ethylene glycol on lethal dose 50 (LD50), urine production, and histopathology change renal tubule cell in mice |
| title_sort | acute toxicity effects of ethylene glycol on lethal dose 50 ld50 urine production and histopathology change renal tubule cell in mice |
| topic | disease eg ld50 renal toxic |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=224812 |
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