Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida
Edwardsiellosis, caused by Edwardsiella piscicida infection in eels (Anguilla japonica), is a major problem for Korean eel farms, so there is an urgent need to develop a vaccine. For both practical and cost reasons, it is preferable to develop an oral vaccine, administration of which is significantl...
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The Korean Society of Fisheries and Aquatic Science
2025-05-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e26 |
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| author | Young Min Woo Keun Woo Lee Byung-Chul Jeong Jae Young Cha Won-Sik Woo Chan-Il Park Young Jae Kim Dong-Geun Lee Sang-Hyeon Lee Jun Cheul Ahn Andre Kim |
| author_facet | Young Min Woo Keun Woo Lee Byung-Chul Jeong Jae Young Cha Won-Sik Woo Chan-Il Park Young Jae Kim Dong-Geun Lee Sang-Hyeon Lee Jun Cheul Ahn Andre Kim |
| author_sort | Young Min Woo |
| collection | DOAJ |
| description | Edwardsiellosis, caused by Edwardsiella piscicida infection in eels (Anguilla japonica), is a major problem for Korean eel farms, so there is an urgent need to develop a vaccine. For both practical and cost reasons, it is preferable to develop an oral vaccine, administration of which is significantly more efficient than injection vaccines. To avoid destruction by gastric enzymes and ensure safe delivery to the intestines, it is essential to apply a coating technology to oral vaccine antigens. To develop an oral vaccine against Edwardsiellosis, liposomes were used as an antigen coating, and the efficacy was evaluated by comparing eels’ mortality rates following oral or injection vaccination and expeirmental infection with E. piscicida. Eels that received the highest concentration oral vaccines (20 mg/fish dosage) experienced only a 6% mortality rate; those that got intraperitoneal injection vaccines had 10% mortality. In addition, the control group mortality rate (46%) was 4.18 times higher than that of the most successful oral vaccine group, confirming the efficacy of the oral vaccine. Antibody agglutination in serum extracted after oral vaccine administration confirmed the efficacy of the oral vaccine: eels that received intraperitoneal (I.P.) injection vaccines had the highest antibody agglutinationvalues (28.25 ± 0.43), but the 20 mg/fish oral vaccine group also showed high antibody agglutination (25.125 ± 1.05). These results conclusively demonstrate that the oral vaccine containing liposome-coated inactivated E. piscicida can generate an immune response sufficient to prevent Edwardsiellosis in eels. |
| format | Article |
| id | doaj-art-69d85ea9e882489cbca9f4e097c59658 |
| institution | Kabale University |
| issn | 2234-1757 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj-art-69d85ea9e882489cbca9f4e097c596582025-08-20T03:44:41ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572025-05-0128529830610.47853/FAS.2025.e26Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicidaYoung Min Woo0Keun Woo Lee1Byung-Chul Jeong2Jae Young Cha3Won-Sik Woo4Chan-Il Park5Young Jae Kim6Dong-Geun Lee7Sang-Hyeon Lee8Jun Cheul Ahn9Andre Kim10Department of Pharmaceutical Engineering, Silla University, Busan 46958, KoreaHANKOOK LIPOSOME, Busan 46702, KoreaCentral R&D Center, KOFEC, Naju 58318, KoreaHANKOOK LIPOSOME, Busan 46702, KoreaInstitute of Marine Industry, College of Marine Science, Gyeongsang National University, Tongyeong 53064, KoreaInstitute of Marine Industry, College of Marine Science, Gyeongsang National University, Tongyeong 53064, KoreaKorea Aquatic Biosecurity Technology, Busan 48564, KoreaDepartment of Pharmaceutical Engineering, Silla University, Busan 46958, KoreaDepartment of Pharmaceutical Engineering, Silla University, Busan 46958, KoreaCentral R&D Center, KOFEC, Naju 58318, KoreaDepartment of Pharmaceutical Engineering, Silla University, Busan 46958, KoreaEdwardsiellosis, caused by Edwardsiella piscicida infection in eels (Anguilla japonica), is a major problem for Korean eel farms, so there is an urgent need to develop a vaccine. For both practical and cost reasons, it is preferable to develop an oral vaccine, administration of which is significantly more efficient than injection vaccines. To avoid destruction by gastric enzymes and ensure safe delivery to the intestines, it is essential to apply a coating technology to oral vaccine antigens. To develop an oral vaccine against Edwardsiellosis, liposomes were used as an antigen coating, and the efficacy was evaluated by comparing eels’ mortality rates following oral or injection vaccination and expeirmental infection with E. piscicida. Eels that received the highest concentration oral vaccines (20 mg/fish dosage) experienced only a 6% mortality rate; those that got intraperitoneal injection vaccines had 10% mortality. In addition, the control group mortality rate (46%) was 4.18 times higher than that of the most successful oral vaccine group, confirming the efficacy of the oral vaccine. Antibody agglutination in serum extracted after oral vaccine administration confirmed the efficacy of the oral vaccine: eels that received intraperitoneal (I.P.) injection vaccines had the highest antibody agglutinationvalues (28.25 ± 0.43), but the 20 mg/fish oral vaccine group also showed high antibody agglutination (25.125 ± 1.05). These results conclusively demonstrate that the oral vaccine containing liposome-coated inactivated E. piscicida can generate an immune response sufficient to prevent Edwardsiellosis in eels. http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e26EdwardsiellosisEdwardsiella piscicidaLiposomeOral vaccineAnguilla japonica |
| spellingShingle | Young Min Woo Keun Woo Lee Byung-Chul Jeong Jae Young Cha Won-Sik Woo Chan-Il Park Young Jae Kim Dong-Geun Lee Sang-Hyeon Lee Jun Cheul Ahn Andre Kim Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida Fisheries and Aquatic Sciences Edwardsiellosis Edwardsiella piscicida Liposome Oral vaccine Anguilla japonica |
| title | Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida |
| title_full | Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida |
| title_fullStr | Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida |
| title_full_unstemmed | Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida |
| title_short | Development and evaluation of an oral liposomal vaccine for the prevention of Edwardsiellosis in Anguilla japonica caused by Edwardsiella piscicida |
| title_sort | development and evaluation of an oral liposomal vaccine for the prevention of edwardsiellosis in anguilla japonica caused by edwardsiella piscicida |
| topic | Edwardsiellosis Edwardsiella piscicida Liposome Oral vaccine Anguilla japonica |
| url | http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e26 |
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