Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells
Summary: GlcNAc-1-phosphotransferase (GNPT) is the key enzyme for tagging lysosomal hydrolases with the mannose 6-phosphate moiety for delivery to the lysosome. Here, we present the assay for measuring endogenous GNPT activity in SK-MEL-30 cells. We provide details for preparing the [3H]UDP-GlcNAc d...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003417 |
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