Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics

Antimicrobial resistance (AMR) is a global threat that occurs as microorganisms evolve to resist antibiotic (ATB) overuse. To monitor this overuse, inexpensive, affordable, and adaptable sensors are required. While electrochemical sensors are promising, they cannot often distinguish between overlapp...

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Main Authors: Sreejesh Moolayadukkam, Priyankan Datta, Dhrubajyoti Chowdhury, Ishwar K. Puri
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Results in Engineering
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590123024016773
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author Sreejesh Moolayadukkam
Priyankan Datta
Dhrubajyoti Chowdhury
Ishwar K. Puri
author_facet Sreejesh Moolayadukkam
Priyankan Datta
Dhrubajyoti Chowdhury
Ishwar K. Puri
author_sort Sreejesh Moolayadukkam
collection DOAJ
description Antimicrobial resistance (AMR) is a global threat that occurs as microorganisms evolve to resist antibiotic (ATB) overuse. To monitor this overuse, inexpensive, affordable, and adaptable sensors are required. While electrochemical sensors are promising, they cannot often distinguish between overlapping electrochemical interactions from multiple ATBs, resulting in poor analyte selectivity. To overcome this challenge, we design electrocatalysts consisting of nickel aluminum layered double hydroxides (NiAl-LDH) and their composites by altering the metal ion ratio to adjust the surface properties and selectivity of sensors. Synthesis of LDH and its graphene oxide (GO) composites is conducted using a low-temperature hydrothermal method. The prepared materials are characterized using various morphological and structural characterization techniques. Electrochemical characterization confirms that tuning the metal ion ratio allows sensors to differentiate between electrochemical reactions, improving the selective detection of amoxicillin and tetracycline from their mixtures. Addition of graphene oxide also enhances sensing capabilities. The optimized sensor has sensitivities of 137.6 nA µM−1 cm−2 and 161.4 nA µM−1 cm−2, and lower detection limits of 4.3 nM and 3.6 nM, respectively, for amoxicillin (Amx) and tetracycline (TC). Interferents have no significant effect on the detection of relatively low concentrations (10 µM) of the two ATBs. The electrodes can also detect the two analytes in tap water samples. This sensor methodology can potentially improve environmental antibiotic detection to mitigate the risk of antimicrobial resistance.
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spelling doaj-art-69bfdf59065f4f03bbe6dac2491a0d9f2025-08-20T02:32:15ZengElsevierResults in Engineering2590-12302024-12-012410342510.1016/j.rineng.2024.103425Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibioticsSreejesh Moolayadukkam0Priyankan Datta1Dhrubajyoti Chowdhury2Ishwar K. Puri3Department of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles CA, 90089, United States; Iovine and Young Academy, University of Southern California, Los Angeles CA, 90089, United States; Corresponding author at: Department of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles CA, 90089, United States; Iovine and Young Academy, University of Southern California, Los Angeles CA, 90089, United States.Department of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles CA, 90089, United StatesDepartment of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles CA, 90089, United States; Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles CA, 90089, United StatesDepartment of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles CA, 90089, United States; Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles CA, 90089, United States; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles CA, 90089, United StatesAntimicrobial resistance (AMR) is a global threat that occurs as microorganisms evolve to resist antibiotic (ATB) overuse. To monitor this overuse, inexpensive, affordable, and adaptable sensors are required. While electrochemical sensors are promising, they cannot often distinguish between overlapping electrochemical interactions from multiple ATBs, resulting in poor analyte selectivity. To overcome this challenge, we design electrocatalysts consisting of nickel aluminum layered double hydroxides (NiAl-LDH) and their composites by altering the metal ion ratio to adjust the surface properties and selectivity of sensors. Synthesis of LDH and its graphene oxide (GO) composites is conducted using a low-temperature hydrothermal method. The prepared materials are characterized using various morphological and structural characterization techniques. Electrochemical characterization confirms that tuning the metal ion ratio allows sensors to differentiate between electrochemical reactions, improving the selective detection of amoxicillin and tetracycline from their mixtures. Addition of graphene oxide also enhances sensing capabilities. The optimized sensor has sensitivities of 137.6 nA µM−1 cm−2 and 161.4 nA µM−1 cm−2, and lower detection limits of 4.3 nM and 3.6 nM, respectively, for amoxicillin (Amx) and tetracycline (TC). Interferents have no significant effect on the detection of relatively low concentrations (10 µM) of the two ATBs. The electrodes can also detect the two analytes in tap water samples. This sensor methodology can potentially improve environmental antibiotic detection to mitigate the risk of antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S2590123024016773Antimicrobial resistanceLayered double hydroxidesGrapheneAntibioticsElectrochemical sensors
spellingShingle Sreejesh Moolayadukkam
Priyankan Datta
Dhrubajyoti Chowdhury
Ishwar K. Puri
Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
Results in Engineering
Antimicrobial resistance
Layered double hydroxides
Graphene
Antibiotics
Electrochemical sensors
title Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
title_full Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
title_fullStr Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
title_full_unstemmed Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
title_short Modifying metal ion ratios in nickel-aluminum layered double hydroxide/reduced graphene oxide composites for selective electrochemical detection of antibiotics
title_sort modifying metal ion ratios in nickel aluminum layered double hydroxide reduced graphene oxide composites for selective electrochemical detection of antibiotics
topic Antimicrobial resistance
Layered double hydroxides
Graphene
Antibiotics
Electrochemical sensors
url http://www.sciencedirect.com/science/article/pii/S2590123024016773
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