Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population
Xing Zhou,1– 3,* Man Yang,1,* Ying Yang,1 Fan Xu,1 Feiying Wang,1 Ming Jiao,2,4 Wenyu Tao,1 Yiping Li1 1Department of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China; 2Kunmi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-02-01
|
| Series: | Pharmacogenomics and Personalized Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/association-of-mirna-polymorphisms-involved-in-the-pi3katkgsk3-pathway-peer-reviewed-fulltext-article-PGPM |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849721542044286976 |
|---|---|
| author | Zhou X Yang M Yang Y Xu F Wang F Jiao M Tao W Li Y |
| author_facet | Zhou X Yang M Yang Y Xu F Wang F Jiao M Tao W Li Y |
| author_sort | Zhou X |
| collection | DOAJ |
| description | Xing Zhou,1– 3,* Man Yang,1,* Ying Yang,1 Fan Xu,1 Feiying Wang,1 Ming Jiao,2,4 Wenyu Tao,1 Yiping Li1 1Department of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China; 2Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 3Department of Endocrinology, Dongguan Tungwah Hospital, Dongguan, Guangdong, People’s Republic of China; 4Yunnan Emergency Center, Kunming, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wenyu Tao; Yiping Li, Department of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China, Email twy623@126.com; yyflyp@aliyun.comBackground: Single nucleotide polymorphisms (SNPs) in miRNA genes can influence the expression of miRNAs that modulate the PI3K/AKT/GSK3β pathway and play crucial roles in type 2 diabetes mellitus (T2DM) susceptibility. The purpose of this study was to investigate the association of SNPs in miRNA genes targeting the PI3K/AKT/GSK3β pathway with T2DM.Methods: This case-control study included 1,416 subjects with T2DM and 1,694 non-diabetics. Eleven SNPs in miRNA genes (rs895819 in miR-27a, rs11888095 in miR-128a, rs2292832 in miR-149, rs6502892 in miR-22, rs13283671 in miR-31, rs1076063 and rs1076064 in miR-378a, rs10061133 in miR-449b, rs3746444 in miR-499a and rs678956 and rs476364 in miR-326) involved in PI3K/AKT/GSK3β pathway were genotyped by TaqMan Genotyping Assay, and the associations of these SNPs with T2DM were analyzed using online SHesis and SNPstats.Results: The results showed that miR-378a rs1076064 G allele could be a protective factor against T2DM (p< 0.001, OR=0.828; 95% CI:0.749– 0.916), whereas the miR-31 rs13283671 C allele could increase the risk of developing T2DM (p=0.003, OR=1.193; 95% CI:1.060– 1.342). In addition, the miR-378a rs1076063A-rs1076064G haplotype could be a protective against T2DM (p< 0.001, OR=0.731; 95% CI:0.649– 0.824). According to inheritance mode analysis, compared with the AA-AG genotype, the GG genotype of rs1076064 showed a protective effect in T2DM in the recessive mode (p< 0.01, OR=0.71; 95% CI: 0.59– 0.84). For rs13283671, compared with the TT genotype, the CT-CC genotype showed a risk effect in T2DM in the dominant inheritance model (p< 0.01, OR=1.29; 95% CI: 1.12– 1.49). Genotype-Tissue Expression (GTEx) Portal database analysis showed that miR-31 rs13283671 CT and CC genotypes had lower AKT expression than TT genotypes.Conclusion: In conclusion, rs13283671 in miR-31 and rs1076064 in miR-378a involved in the PI3K/AKT/GSK3β pathway were associated with T2DM susceptibility in a Chinese population.Keywords: T2DM, GSK3β, microRNA, polymorphisms, Chinese population |
| format | Article |
| id | doaj-art-69bbba8b50074f9cb634d40d5ca66757 |
| institution | DOAJ |
| issn | 1178-7066 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Pharmacogenomics and Personalized Medicine |
| spelling | doaj-art-69bbba8b50074f9cb634d40d5ca667572025-08-20T03:11:37ZengDove Medical PressPharmacogenomics and Personalized Medicine1178-70662025-02-01Volume 187184100163Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese PopulationZhou XYang MYang YXu FWang FJiao MTao WLi YXing Zhou,1– 3,* Man Yang,1,* Ying Yang,1 Fan Xu,1 Feiying Wang,1 Ming Jiao,2,4 Wenyu Tao,1 Yiping Li1 1Department of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China; 2Kunming Medical University, Kunming, Yunnan, People’s Republic of China; 3Department of Endocrinology, Dongguan Tungwah Hospital, Dongguan, Guangdong, People’s Republic of China; 4Yunnan Emergency Center, Kunming, Yunnan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wenyu Tao; Yiping Li, Department of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China, Email twy623@126.com; yyflyp@aliyun.comBackground: Single nucleotide polymorphisms (SNPs) in miRNA genes can influence the expression of miRNAs that modulate the PI3K/AKT/GSK3β pathway and play crucial roles in type 2 diabetes mellitus (T2DM) susceptibility. The purpose of this study was to investigate the association of SNPs in miRNA genes targeting the PI3K/AKT/GSK3β pathway with T2DM.Methods: This case-control study included 1,416 subjects with T2DM and 1,694 non-diabetics. Eleven SNPs in miRNA genes (rs895819 in miR-27a, rs11888095 in miR-128a, rs2292832 in miR-149, rs6502892 in miR-22, rs13283671 in miR-31, rs1076063 and rs1076064 in miR-378a, rs10061133 in miR-449b, rs3746444 in miR-499a and rs678956 and rs476364 in miR-326) involved in PI3K/AKT/GSK3β pathway were genotyped by TaqMan Genotyping Assay, and the associations of these SNPs with T2DM were analyzed using online SHesis and SNPstats.Results: The results showed that miR-378a rs1076064 G allele could be a protective factor against T2DM (p< 0.001, OR=0.828; 95% CI:0.749– 0.916), whereas the miR-31 rs13283671 C allele could increase the risk of developing T2DM (p=0.003, OR=1.193; 95% CI:1.060– 1.342). In addition, the miR-378a rs1076063A-rs1076064G haplotype could be a protective against T2DM (p< 0.001, OR=0.731; 95% CI:0.649– 0.824). According to inheritance mode analysis, compared with the AA-AG genotype, the GG genotype of rs1076064 showed a protective effect in T2DM in the recessive mode (p< 0.01, OR=0.71; 95% CI: 0.59– 0.84). For rs13283671, compared with the TT genotype, the CT-CC genotype showed a risk effect in T2DM in the dominant inheritance model (p< 0.01, OR=1.29; 95% CI: 1.12– 1.49). Genotype-Tissue Expression (GTEx) Portal database analysis showed that miR-31 rs13283671 CT and CC genotypes had lower AKT expression than TT genotypes.Conclusion: In conclusion, rs13283671 in miR-31 and rs1076064 in miR-378a involved in the PI3K/AKT/GSK3β pathway were associated with T2DM susceptibility in a Chinese population.Keywords: T2DM, GSK3β, microRNA, polymorphisms, Chinese populationhttps://www.dovepress.com/association-of-mirna-polymorphisms-involved-in-the-pi3katkgsk3-pathway-peer-reviewed-fulltext-article-PGPMt2dmgsk3βmicrornapolymorphismschinese population. |
| spellingShingle | Zhou X Yang M Yang Y Xu F Wang F Jiao M Tao W Li Y Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population Pharmacogenomics and Personalized Medicine t2dm gsk3β microrna polymorphisms chinese population. |
| title | Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population |
| title_full | Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population |
| title_fullStr | Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population |
| title_full_unstemmed | Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population |
| title_short | Association of MiRNA Polymorphisms Involved in the PI3K/ATK/GSK3β Pathway with T2DM in a Chinese Population |
| title_sort | association of mirna polymorphisms involved in the pi3k atk gsk3 beta pathway with t2dm in a chinese population |
| topic | t2dm gsk3β microrna polymorphisms chinese population. |
| url | https://www.dovepress.com/association-of-mirna-polymorphisms-involved-in-the-pi3katkgsk3-pathway-peer-reviewed-fulltext-article-PGPM |
| work_keys_str_mv | AT zhoux associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT yangm associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT yangy associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT xuf associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT wangf associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT jiaom associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT taow associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation AT liy associationofmirnapolymorphismsinvolvedinthepi3katkgsk3betapathwaywitht2dminachinesepopulation |