Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells
Triple‐negative breast cancer (TNBC) faces significant treatment challenges due to its resistance to chemotherapy and high‐rate metastatic occurrence. Adjunctive treatment is a promising approach to improve chemotherapy effectiveness while reducing toxicity on normal cells. Natural compounds of Lans...
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| Format: | Article |
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Universitas Gadjah Mada, Yogyakarta
2025-03-01
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| Series: | Indonesian Journal of Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/102112 |
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| author | Faaza Aulia Rahman Aulia Nur Septiani Desty Restia Rahmawati Novia Permata Hapsari Edy Meiyanto Ratna Asmah Susidarti |
| author_facet | Faaza Aulia Rahman Aulia Nur Septiani Desty Restia Rahmawati Novia Permata Hapsari Edy Meiyanto Ratna Asmah Susidarti |
| author_sort | Faaza Aulia Rahman |
| collection | DOAJ |
| description | Triple‐negative breast cancer (TNBC) faces significant treatment challenges due to its resistance to chemotherapy and high‐rate metastatic occurrence. Adjunctive treatment is a promising approach to improve chemotherapy effectiveness while reducing toxicity on normal cells. Natural compounds of Lansium domesticum (duku) exhibit potential as adjunctive cancer treatments. This study aims to investigate the potential of duku peel extract (DPE) to be developed as an adjunctive agent. The cytotoxic activity of DPE against 4T1 cells was conducted through MTT assay, both in single and combination treatments. The anti‐migratory effect of DPE was examined by scratch wound healing assay. The molecular mechanism of DPE was confirmed using virtual screening via bioinformatics approaches, including protein target prediction and molecular docking. The results show that DPE has cytotoxic activity with an IC50 value of 47 µg/mL against the 4T1 cell line, in a 24‐hour treatment period. Interestingly, DPE not only had a good synergistic effect (mean CI value, i.e. 0.34), but also showed significant inhibition of cell migration with doxorubicin (Dox). Additionally, virtual bioinformatics screening approaches suggest the potential mechanism of DPE compound action by targeting CDC25B and TOP2A. Overall, DPE holds promise as an adjunctive treatment of Dox against 4T1 TNBC cells. |
| format | Article |
| id | doaj-art-69b8f2f6d64045a1a4e5e9d985007521 |
| institution | DOAJ |
| issn | 0853-8654 2089-2241 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj-art-69b8f2f6d64045a1a4e5e9d9850075212025-08-20T03:08:20ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412025-03-0130110.22146/ijbiotech.10211237332Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cellsFaaza Aulia Rahman0Aulia Nur Septiani1Desty Restia Rahmawati2Novia Permata Hapsari3Edy Meiyanto4Ratna Asmah Susidarti5Cancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, IndonesiaCancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, IndonesiaCancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, IndonesiaCancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, IndonesiaLaboratory of Macromolecular Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, IndonesiaCancer Chemoprevention Research Center (CCRC) Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Indonesia; Laboratory of Medicinal Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, IndonesiaTriple‐negative breast cancer (TNBC) faces significant treatment challenges due to its resistance to chemotherapy and high‐rate metastatic occurrence. Adjunctive treatment is a promising approach to improve chemotherapy effectiveness while reducing toxicity on normal cells. Natural compounds of Lansium domesticum (duku) exhibit potential as adjunctive cancer treatments. This study aims to investigate the potential of duku peel extract (DPE) to be developed as an adjunctive agent. The cytotoxic activity of DPE against 4T1 cells was conducted through MTT assay, both in single and combination treatments. The anti‐migratory effect of DPE was examined by scratch wound healing assay. The molecular mechanism of DPE was confirmed using virtual screening via bioinformatics approaches, including protein target prediction and molecular docking. The results show that DPE has cytotoxic activity with an IC50 value of 47 µg/mL against the 4T1 cell line, in a 24‐hour treatment period. Interestingly, DPE not only had a good synergistic effect (mean CI value, i.e. 0.34), but also showed significant inhibition of cell migration with doxorubicin (Dox). Additionally, virtual bioinformatics screening approaches suggest the potential mechanism of DPE compound action by targeting CDC25B and TOP2A. Overall, DPE holds promise as an adjunctive treatment of Dox against 4T1 TNBC cells.https://jurnal.ugm.ac.id/ijbiotech/article/view/102112adjunctive treatmentbioinformaticslansium domesticummigrationtnbc |
| spellingShingle | Faaza Aulia Rahman Aulia Nur Septiani Desty Restia Rahmawati Novia Permata Hapsari Edy Meiyanto Ratna Asmah Susidarti Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells Indonesian Journal of Biotechnology adjunctive treatment bioinformatics lansium domesticum migration tnbc |
| title | Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells |
| title_full | Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells |
| title_fullStr | Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells |
| title_full_unstemmed | Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells |
| title_short | Duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4T1 cells |
| title_sort | duku peel ethyl acetate extract as an adjunctive treatment of doxorubicin on triple negative breast cancer 4t1 cells |
| topic | adjunctive treatment bioinformatics lansium domesticum migration tnbc |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/102112 |
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