Comprehensive Evaluation of <i>Nanhaia speciosa</i> Germplasm Resources Using Agronomic Traits, Molecular Markers, and Metabolomics
<i>Nanhaia speciosa</i> is a valuable industrial crop known for its significant edible and medicinal properties, attributed to its abundance of secondary metabolites. This study aims to elucidate the genetic relationships among <i>N. speciosa</i>, enhance species identificati...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/3/508 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Nanhaia speciosa</i> is a valuable industrial crop known for its significant edible and medicinal properties, attributed to its abundance of secondary metabolites. This study aims to elucidate the genetic relationships among <i>N. speciosa</i>, enhance species identification accuracy, and select genetically stable, high-quality germplasms of <i>N. speciosa</i>. To achieve this, we conducted chloroplast gene amplification and sequencing, alongside an analysis of the agronomic traits of 19 <i>N. speciosa</i> germplasms. Additionally, non-targeted metabolomic profiling using UPLC-Q-Orbitrap/MS and chemometric methods was employed to assess their metabolic diversity and genetic relationships. The results revealed notable differences in DNA barcodes and secondary structures across the germplasms, with the <i>atp</i>F-<i>atp</i>H sequence successfully distinguishing all 19 germplasms into distinct varieties. Multivariate statistical analyses, including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and hierarchical clustering, identified 12 key marker metabolites that effectively differentiated the germplasms, consistent with the observed phenotypic diversity. A clustering analysis integrating genetic, phenotypic, and metabolomic data further validated the utility of DNA barcoding for species identification. The results of the comprehensive analysis showed that NDL-2 and NDL-18 exhibited relatively good edible and medicinal properties, while NDL-1 and NDL-19 exhibited relatively poor edible and medicinal properties. This study provides crucial insights for the breeding and genetic improvement of <i>N. speciosa</i> and related species, offering a valuable reference for the identification, conservation, and utilization of germplasm resources within the <i>N. speciosa</i>. |
|---|---|
| ISSN: | 2073-4395 |