CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report
CD4+CD25highCD127low/−FoxP3+ regulatory T cells (Tregs) are currently under extensive investigation in childhood acute lymphoblastic leukemia (ALL) and in other human cancers. Usually, Treg cells maintain the immune cell homeostasis. This small subset of T cells has been, in fact, considered to be i...
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| Format: | Article |
| Language: | English |
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2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/1292404 |
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| author | M. Niedźwiecki O. Budziło M. Zieliński E. Adamkiewicz-Drożyńska L. Maciejka-Kembłowska T. Szczepański P. Trzonkowski |
| author_facet | M. Niedźwiecki O. Budziło M. Zieliński E. Adamkiewicz-Drożyńska L. Maciejka-Kembłowska T. Szczepański P. Trzonkowski |
| author_sort | M. Niedźwiecki |
| collection | DOAJ |
| description | CD4+CD25highCD127low/−FoxP3+ regulatory T cells (Tregs) are currently under extensive investigation in childhood acute lymphoblastic leukemia (ALL) and in other human cancers. Usually, Treg cells maintain the immune cell homeostasis. This small subset of T cells has been, in fact, considered to be involved in the pathogenesis of autoimmune diseases and progression of acute and chronic leukemias. However, whether Treg dysregulation in CLL and ALL plays a key role or it rather represents a simple epiphenomenon is still a matter of debate. Treg cells have been proposed as a prognostic indicator of the clinical course of the disease and might also be used for targeted immune therapy. Our study revealed statistically higher percentage of Treg cells in the bone marrow than in peripheral blood in the group of 42 children with acute lymphoblastic leukemia. By analyzing Treg subpopulations, it was shown that only memory Tregs in contact with leukemic antigens showed statistically significant differences. We noticed a low negative correlation between Treg cells in the bone marrow and the percentage of blasts (R=−0.36) as well as a moderate correlation between Treg cells in the bone marrow and Hb level (R=+0.41) in peripheral blood before therapy. The number of peripheral blood blasts on day 8th correlates negatively (R=−0.36) with Tregs. Furthermore, statistical analysis revealed low negative correlation between the number of Tregs in the bone marrow and the minimal residual disease measured on day 15th, the percentage of blasts in the bone marrow and leukocytosis after 15 days of chemotherapy. These results indicate the influence of Tregs on the final therapeutic effect. |
| format | Article |
| id | doaj-art-69ab7482393544b6a958f4b620101d1b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-69ab7482393544b6a958f4b620101d1b2025-02-03T05:58:11ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/12924041292404CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief ReportM. Niedźwiecki0O. Budziło1M. Zieliński2E. Adamkiewicz-Drożyńska3L. Maciejka-Kembłowska4T. Szczepański5P. Trzonkowski6Department of Pediatrics, Hematology and Oncology, Medical University of Gdansk, Gdańsk, PolandDepartment of Pediatrics, Hematology and Oncology, Medical University of Gdansk, Gdańsk, PolandClinical Immunology and Transplantology Unit at the Department of Immunology, Medical University of Gdansk, Gdańsk, PolandDepartment of Pediatrics, Hematology and Oncology, Medical University of Gdansk, Gdańsk, PolandDepartment of Pediatrics, Hematology and Oncology, Medical University of Gdansk, Gdańsk, PolandDepartment of Pediatric, Hematology and Oncology, Zabrze Medical University of Silesia, Katowice, PolandClinical Immunology and Transplantology Unit at the Department of Immunology, Medical University of Gdansk, Gdańsk, PolandCD4+CD25highCD127low/−FoxP3+ regulatory T cells (Tregs) are currently under extensive investigation in childhood acute lymphoblastic leukemia (ALL) and in other human cancers. Usually, Treg cells maintain the immune cell homeostasis. This small subset of T cells has been, in fact, considered to be involved in the pathogenesis of autoimmune diseases and progression of acute and chronic leukemias. However, whether Treg dysregulation in CLL and ALL plays a key role or it rather represents a simple epiphenomenon is still a matter of debate. Treg cells have been proposed as a prognostic indicator of the clinical course of the disease and might also be used for targeted immune therapy. Our study revealed statistically higher percentage of Treg cells in the bone marrow than in peripheral blood in the group of 42 children with acute lymphoblastic leukemia. By analyzing Treg subpopulations, it was shown that only memory Tregs in contact with leukemic antigens showed statistically significant differences. We noticed a low negative correlation between Treg cells in the bone marrow and the percentage of blasts (R=−0.36) as well as a moderate correlation between Treg cells in the bone marrow and Hb level (R=+0.41) in peripheral blood before therapy. The number of peripheral blood blasts on day 8th correlates negatively (R=−0.36) with Tregs. Furthermore, statistical analysis revealed low negative correlation between the number of Tregs in the bone marrow and the minimal residual disease measured on day 15th, the percentage of blasts in the bone marrow and leukocytosis after 15 days of chemotherapy. These results indicate the influence of Tregs on the final therapeutic effect.http://dx.doi.org/10.1155/2018/1292404 |
| spellingShingle | M. Niedźwiecki O. Budziło M. Zieliński E. Adamkiewicz-Drożyńska L. Maciejka-Kembłowska T. Szczepański P. Trzonkowski CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report Journal of Immunology Research |
| title | CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report |
| title_full | CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report |
| title_fullStr | CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report |
| title_full_unstemmed | CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report |
| title_short | CD4+CD25highCD127low/−FoxP3+ Regulatory T Cell Subpopulations in the Bone Marrow and Peripheral Blood of Children with ALL: Brief Report |
| title_sort | cd4 cd25highcd127low foxp3 regulatory t cell subpopulations in the bone marrow and peripheral blood of children with all brief report |
| url | http://dx.doi.org/10.1155/2018/1292404 |
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