Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments
In this study, active secondary metabolite production capacity of actinomycete isolates obtained from Trabzon (Black Sea) sea sediments was investigated by molecular techniques. Totaly 24 actinomycetes were investigated by PCR based on the presence of secondary metabolite biosynthesis genes PKS / NR...
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| Language: | English |
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Hasan Eleroğlu
2017-06-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1068 |
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| author | Kadriye Özcan |
| author_facet | Kadriye Özcan |
| author_sort | Kadriye Özcan |
| collection | DOAJ |
| description | In this study, active secondary metabolite production capacity of actinomycete isolates obtained from Trabzon (Black Sea) sea sediments was investigated by molecular techniques. Totaly 24 actinomycetes were investigated by PCR based on the presence of secondary metabolite biosynthesis genes PKS / NRPS. According to the PCR results, 25 and 58% of actinomycetes obtained from Trabzon sea sediments were found to contain PKS-NRPS and only NRPS gene regions, respectively. When PCR data were evaluated, it was found that the production of the peptide form active secondary metabolite of the isolates by non-ribosomal way was higher than that of the secondary metabolite production by the PKS pathway. In addition, it has been determined that Black Sea marine sediments have high potential for active secondary metabolite production. |
| format | Article |
| id | doaj-art-69aa4bb939554f1f97ae394a87a9e9dd |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-69aa4bb939554f1f97ae394a87a9e9dd2025-08-20T01:57:35ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2017-06-015550250610.24925/turjaf.v5i5.502-506.1068530Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sedimentsKadriye Özcan0Giresun Üniversitesi, Mühendislik Fakültesi, Genetik ve Biyomühendislik Bölümü, 28200, TürkiyeIn this study, active secondary metabolite production capacity of actinomycete isolates obtained from Trabzon (Black Sea) sea sediments was investigated by molecular techniques. Totaly 24 actinomycetes were investigated by PCR based on the presence of secondary metabolite biosynthesis genes PKS / NRPS. According to the PCR results, 25 and 58% of actinomycetes obtained from Trabzon sea sediments were found to contain PKS-NRPS and only NRPS gene regions, respectively. When PCR data were evaluated, it was found that the production of the peptide form active secondary metabolite of the isolates by non-ribosomal way was higher than that of the secondary metabolite production by the PKS pathway. In addition, it has been determined that Black Sea marine sediments have high potential for active secondary metabolite production.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1068PCRPKSNRPSAktinomisetSekonder metabolit |
| spellingShingle | Kadriye Özcan Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments Turkish Journal of Agriculture: Food Science and Technology PCR PKS NRPS Aktinomiset Sekonder metabolit |
| title | Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments |
| title_full | Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments |
| title_fullStr | Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments |
| title_full_unstemmed | Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments |
| title_short | Screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from Trabzon (Black Sea) sea sediments |
| title_sort | screening of secondary metabolite biosynthesis genes of marine actinomycetes isolated from trabzon black sea sea sediments |
| topic | PCR PKS NRPS Aktinomiset Sekonder metabolit |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1068 |
| work_keys_str_mv | AT kadriyeozcan screeningofsecondarymetabolitebiosynthesisgenesofmarineactinomycetesisolatedfromtrabzonblackseaseasediments |