Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model
We present an ultrasonography (US)-magnetic resonance imaging (MRI) coregistration technique and examine its application in a preliminary multimodal, multiparametric study in a preclinical model of breast cancer. Nine mice were injected with 67NR breast cancer cells and imaged 6 and 9 days later wit...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2009-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2009.00018 |
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| author | Mary E. Loveless Jennifer G. Whisenant Kevin Wilson Andrej Lyshchik Tuhin K. Sinha John C. Gore Thomas E. Yankeelov |
| author_facet | Mary E. Loveless Jennifer G. Whisenant Kevin Wilson Andrej Lyshchik Tuhin K. Sinha John C. Gore Thomas E. Yankeelov |
| author_sort | Mary E. Loveless |
| collection | DOAJ |
| description | We present an ultrasonography (US)-magnetic resonance imaging (MRI) coregistration technique and examine its application in a preliminary multimodal, multiparametric study in a preclinical model of breast cancer. Nine mice were injected with 67NR breast cancer cells and imaged 6 and 9 days later with 4.7 T MRI and high-frequency US. Tumor volumes from each data set were segmented independently by two investigators and coregistered using an iterative closest point algorithm. In addition to anatomic images, vascular endothelial growth factor receptor 2 (VEGFR2) distribution images from the central tumor slice using VEGFR2-targeted ultrasound contrast agent (UCA) and measurements of perfusion and extravascular-extracellular volume fraction using dynamic contrast-enhanced MRI were acquired from five mice for multiparametric coregistration. Parametric maps from each modality were coregistered and examined for spatial correlation. Average registration root mean square (RMS) error was 0.36 ± 0.11 mm, less than approximately two voxels. Segmented volumes were compared between investigators to minimize interobserver variability; the average RMS error was 0.23 ± 0.09 mm. In the preliminary study, VEGFR2-targeted UCA data did not demonstrate direct spatial correlation with magnetic resonance measures of vascular properties. In summary, a method for accurately coregistering small animal US and MRI has been presented that allows for comparison of quantitative metrics provided by the two modalities. |
| format | Article |
| id | doaj-art-69a6e8dc873d490b912ae34753302db8 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2009-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-69a6e8dc873d490b912ae34753302db82025-01-03T01:22:46ZengSAGE PublishingMolecular Imaging1536-01212009-07-01810.2310/7290.2009.0001810.2310_7290.2009.00018Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor ModelMary E. LovelessJennifer G. WhisenantKevin WilsonAndrej LyshchikTuhin K. SinhaJohn C. GoreThomas E. YankeelovWe present an ultrasonography (US)-magnetic resonance imaging (MRI) coregistration technique and examine its application in a preliminary multimodal, multiparametric study in a preclinical model of breast cancer. Nine mice were injected with 67NR breast cancer cells and imaged 6 and 9 days later with 4.7 T MRI and high-frequency US. Tumor volumes from each data set were segmented independently by two investigators and coregistered using an iterative closest point algorithm. In addition to anatomic images, vascular endothelial growth factor receptor 2 (VEGFR2) distribution images from the central tumor slice using VEGFR2-targeted ultrasound contrast agent (UCA) and measurements of perfusion and extravascular-extracellular volume fraction using dynamic contrast-enhanced MRI were acquired from five mice for multiparametric coregistration. Parametric maps from each modality were coregistered and examined for spatial correlation. Average registration root mean square (RMS) error was 0.36 ± 0.11 mm, less than approximately two voxels. Segmented volumes were compared between investigators to minimize interobserver variability; the average RMS error was 0.23 ± 0.09 mm. In the preliminary study, VEGFR2-targeted UCA data did not demonstrate direct spatial correlation with magnetic resonance measures of vascular properties. In summary, a method for accurately coregistering small animal US and MRI has been presented that allows for comparison of quantitative metrics provided by the two modalities.https://doi.org/10.2310/7290.2009.00018 |
| spellingShingle | Mary E. Loveless Jennifer G. Whisenant Kevin Wilson Andrej Lyshchik Tuhin K. Sinha John C. Gore Thomas E. Yankeelov Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model Molecular Imaging |
| title | Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model |
| title_full | Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model |
| title_fullStr | Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model |
| title_full_unstemmed | Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model |
| title_short | Coregistration of Ultrasonography and Magnetic Resonance Imaging with a Preliminary Investigation of the Spatial Colocalization of Vascular Endothelial Growth Factor Receptor 2 Expression and Tumor Perfusion in a Murine Tumor Model |
| title_sort | coregistration of ultrasonography and magnetic resonance imaging with a preliminary investigation of the spatial colocalization of vascular endothelial growth factor receptor 2 expression and tumor perfusion in a murine tumor model |
| url | https://doi.org/10.2310/7290.2009.00018 |
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