Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry
A Simple high-performance thin layer chromatography (HPTLC) method for separation and quantitative analysis of losartan potassium, amlodipine, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard so...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/108281 |
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| author | Karunanidhi Santhana Lakshmi Sivasubramanian Lakshmi |
| author_facet | Karunanidhi Santhana Lakshmi Sivasubramanian Lakshmi |
| author_sort | Karunanidhi Santhana Lakshmi |
| collection | DOAJ |
| description | A Simple high-performance thin layer chromatography (HPTLC) method for separation and quantitative analysis of losartan potassium, amlodipine, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard solutions were applied to silica gel plates and developed with chloroform : methanol : acetone : formic acid 7.5 : 1.3 : 0.5 : 0.03 (𝑣/𝑣/𝑣/𝑣) as mobile phase. Zones were scanned densitometrically at 254 nm. The 𝑅𝑓 values of amlodipine besylate, hydrochlorothiazide, and losartan potassium were 0.35, 0.57, and 0.74, respectively. Calibration plots were linear in the ranges 500–3000 ng per spot for losartan potassium, amlodipine and hydrochlorothiazide, the correlation coefficients, r, were 0.998, 0.998, and 0.999, respectively. The suitability of this method for quantitative determination of these compounds was by validation in accordance with the requirements of pharmaceutical regulatory standards. The method can be used for routine analysis of these drugs in bulk and in formulation. |
| format | Article |
| id | doaj-art-69a0aa5753234a2f956740634cd0495c |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-69a0aa5753234a2f956740634cd0495c2025-08-20T03:34:05ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732012-01-01201210.1155/2012/108281108281Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption DensitometryKarunanidhi Santhana Lakshmi0Sivasubramanian Lakshmi1Department of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Tamilnadu, Kattankulathur 603 203, IndiaDepartment of Pharmaceutical Analysis, SRM College of Pharmacy, SRM University, Tamilnadu, Kattankulathur 603 203, IndiaA Simple high-performance thin layer chromatography (HPTLC) method for separation and quantitative analysis of losartan potassium, amlodipine, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated. After extraction with methanol, sample and standard solutions were applied to silica gel plates and developed with chloroform : methanol : acetone : formic acid 7.5 : 1.3 : 0.5 : 0.03 (𝑣/𝑣/𝑣/𝑣) as mobile phase. Zones were scanned densitometrically at 254 nm. The 𝑅𝑓 values of amlodipine besylate, hydrochlorothiazide, and losartan potassium were 0.35, 0.57, and 0.74, respectively. Calibration plots were linear in the ranges 500–3000 ng per spot for losartan potassium, amlodipine and hydrochlorothiazide, the correlation coefficients, r, were 0.998, 0.998, and 0.999, respectively. The suitability of this method for quantitative determination of these compounds was by validation in accordance with the requirements of pharmaceutical regulatory standards. The method can be used for routine analysis of these drugs in bulk and in formulation.http://dx.doi.org/10.1155/2012/108281 |
| spellingShingle | Karunanidhi Santhana Lakshmi Sivasubramanian Lakshmi Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry Journal of Analytical Methods in Chemistry |
| title | Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry |
| title_full | Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry |
| title_fullStr | Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry |
| title_full_unstemmed | Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry |
| title_short | Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry |
| title_sort | simultaneous analysis of losartan potassium amlodipine besylate and hydrochlorothiazide in bulk and in tablets by high performance thin layer chromatography with uv absorption densitometry |
| url | http://dx.doi.org/10.1155/2012/108281 |
| work_keys_str_mv | AT karunanidhisanthanalakshmi simultaneousanalysisoflosartanpotassiumamlodipinebesylateandhydrochlorothiazideinbulkandintabletsbyhighperformancethinlayerchromatographywithuvabsorptiondensitometry AT sivasubramanianlakshmi simultaneousanalysisoflosartanpotassiumamlodipinebesylateandhydrochlorothiazideinbulkandintabletsbyhighperformancethinlayerchromatographywithuvabsorptiondensitometry |