IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus.
Persistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248639&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850181333587853312 |
|---|---|
| author | Camila Mareti Bonin-Jacob Larissa Zatorre Almeida-Lugo Marco Antonio Moreira Puga Ana Paula Machado Cacilda Tezelli Junqueira Padovani Mariana Calarge Noceti Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Júlio César Possati Resende Adriane Cristina Bovo Inês Aparecida Tozetti |
| author_facet | Camila Mareti Bonin-Jacob Larissa Zatorre Almeida-Lugo Marco Antonio Moreira Puga Ana Paula Machado Cacilda Tezelli Junqueira Padovani Mariana Calarge Noceti Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Júlio César Possati Resende Adriane Cristina Bovo Inês Aparecida Tozetti |
| author_sort | Camila Mareti Bonin-Jacob |
| collection | DOAJ |
| description | Persistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-4, and IL-2 were quantified in the serum and exfoliated cervical cells (ECCs) of patients with HR-HPV, and the presence of IL-6+ cells was investigated in uterine cervix biopsies. Cytokine levels in the serum and ECCs of 26 HR-HPV DNA-positive patients and 18 HPV DNA-negative patients were measured using flow cytometry. Fifteen uterine cervix biopsy samples embedded in paraffin were subjected to immunohistochemical analysis for the detection of IL-6+ cells. HR-HPV-positive patients showed increased IL-6 and IL-10 in the ECCs and serum, respectively. Compared with HPV DNA-positive patients, HPV DNA-negative patients had higher levels of IL-6 in ECCs. Patients with multiple infections of HPV had higher levels of IL-6 in their ECCs than those with a single infection. Immunostaining of uterine cervix biopsy samples revealed no differences in IL-6 expression between the different classes of histopathological lesions. However, differences were observed in the expression levels of IL-6 and IL-10 at the systemic and local levels in HR-HPV-positive patients without cervical lesions. Considering the functional characteristics of these cytokines, it can be inferred that such patients are prone to persistent HPV infection. |
| format | Article |
| id | doaj-art-6998a7ee2b1c4c90bb8aea37b2679cab |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-6998a7ee2b1c4c90bb8aea37b2679cab2025-08-20T02:17:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024863910.1371/journal.pone.0248639IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus.Camila Mareti Bonin-JacobLarissa Zatorre Almeida-LugoMarco Antonio Moreira PugaAna Paula MachadoCacilda Tezelli Junqueira PadovaniMariana Calarge NocetiAlda Maria Teixeira FerreiraCarlos Eurico Dos Santos FernandesJúlio César Possati ResendeAdriane Cristina BovoInês Aparecida TozettiPersistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-4, and IL-2 were quantified in the serum and exfoliated cervical cells (ECCs) of patients with HR-HPV, and the presence of IL-6+ cells was investigated in uterine cervix biopsies. Cytokine levels in the serum and ECCs of 26 HR-HPV DNA-positive patients and 18 HPV DNA-negative patients were measured using flow cytometry. Fifteen uterine cervix biopsy samples embedded in paraffin were subjected to immunohistochemical analysis for the detection of IL-6+ cells. HR-HPV-positive patients showed increased IL-6 and IL-10 in the ECCs and serum, respectively. Compared with HPV DNA-positive patients, HPV DNA-negative patients had higher levels of IL-6 in ECCs. Patients with multiple infections of HPV had higher levels of IL-6 in their ECCs than those with a single infection. Immunostaining of uterine cervix biopsy samples revealed no differences in IL-6 expression between the different classes of histopathological lesions. However, differences were observed in the expression levels of IL-6 and IL-10 at the systemic and local levels in HR-HPV-positive patients without cervical lesions. Considering the functional characteristics of these cytokines, it can be inferred that such patients are prone to persistent HPV infection.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248639&type=printable |
| spellingShingle | Camila Mareti Bonin-Jacob Larissa Zatorre Almeida-Lugo Marco Antonio Moreira Puga Ana Paula Machado Cacilda Tezelli Junqueira Padovani Mariana Calarge Noceti Alda Maria Teixeira Ferreira Carlos Eurico Dos Santos Fernandes Júlio César Possati Resende Adriane Cristina Bovo Inês Aparecida Tozetti IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. PLoS ONE |
| title | IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. |
| title_full | IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. |
| title_fullStr | IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. |
| title_full_unstemmed | IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. |
| title_short | IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus. |
| title_sort | il 6 and il 10 in the serum and exfoliated cervical cells of patients infected with high risk human papillomavirus |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248639&type=printable |
| work_keys_str_mv | AT camilamaretiboninjacob il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT larissazatorrealmeidalugo il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT marcoantoniomoreirapuga il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT anapaulamachado il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT cacildatezellijunqueirapadovani il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT marianacalargenoceti il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT aldamariateixeiraferreira il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT carloseuricodossantosfernandes il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT juliocesarpossatiresende il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT adrianecristinabovo il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus AT inesaparecidatozetti il6andil10intheserumandexfoliatedcervicalcellsofpatientsinfectedwithhighriskhumanpapillomavirus |