Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila
Aging decreases muscle mass, strength, and functional capacity. High-salt stress seems to promote muscle aging and decrease lifespan. However, exercise delays muscle aging and increases longevity, and it may protect muscle from rapid aging induced by high-salt intake (HSI), but the molecular mechani...
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| Format: | Article |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000551 |
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| author | Dengtai Wen Yiling Chen Xu Tian Wenqi Hou |
| author_facet | Dengtai Wen Yiling Chen Xu Tian Wenqi Hou |
| author_sort | Dengtai Wen |
| collection | DOAJ |
| description | Aging decreases muscle mass, strength, and functional capacity. High-salt stress seems to promote muscle aging and decrease lifespan. However, exercise delays muscle aging and increases longevity, and it may protect muscle from rapid aging induced by high-salt intake (HSI), but the molecular mechanisms are poorly understood. In this study, the flies were fed a high-salt diet and trained to exercise. Muscle CG2196 (salt) gene and dSir2 gene were over-expressed by building mef2-gal4/UAS system. The results showed that both physical exercise and muscle dSir2 gene overexpression prevented HSI-induced and muscle salt overexpression-induced accelerated age-related decline of climbing index, climbing endurance, muscle NAD+ level, SOD activity level, dSir2 expression, and dFOXO expression, and they also prevented HSI-induced and muscle salt overexpression-induced accelerated age-related increase in muscle ROS level, MDA level, and salt gene expression. Physical exercise improved lifespan decrease induced by HSI and muscle salt overexpression. Therefore, current results indicated that high-salt stress accelerated muscle aging by decreasing muscular NAD+/dSir2/dFOXO pathway activity and increasing oxidative stress. Physical exercise protected muscle from accelerated aging induced by high-salt stress through activating muscle NAD+/dSir2/dFOXO pathway and enhancing muscle oxidation resistance. The combination of exercise and muscle dSir2 overexpression had the best protective effect on muscle aging and lifespan in flies. |
| format | Article |
| id | doaj-art-6995673c26154d3c8bec655a50dae859 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6995673c26154d3c8bec655a50dae8592025-02-03T06:52:48ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112619481960Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in DrosophilaDengtai Wen0Yiling Chen1Xu Tian2Wenqi Hou3Department of Physical Education, Ludong University, Yantai 264025, China; Corresponding authors.College of Physical Education, Yichun University, Yichun 336000, China; Corresponding authors.School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China; Corresponding authors.Department of Physical Education, Ludong University, Yantai 264025, ChinaAging decreases muscle mass, strength, and functional capacity. High-salt stress seems to promote muscle aging and decrease lifespan. However, exercise delays muscle aging and increases longevity, and it may protect muscle from rapid aging induced by high-salt intake (HSI), but the molecular mechanisms are poorly understood. In this study, the flies were fed a high-salt diet and trained to exercise. Muscle CG2196 (salt) gene and dSir2 gene were over-expressed by building mef2-gal4/UAS system. The results showed that both physical exercise and muscle dSir2 gene overexpression prevented HSI-induced and muscle salt overexpression-induced accelerated age-related decline of climbing index, climbing endurance, muscle NAD+ level, SOD activity level, dSir2 expression, and dFOXO expression, and they also prevented HSI-induced and muscle salt overexpression-induced accelerated age-related increase in muscle ROS level, MDA level, and salt gene expression. Physical exercise improved lifespan decrease induced by HSI and muscle salt overexpression. Therefore, current results indicated that high-salt stress accelerated muscle aging by decreasing muscular NAD+/dSir2/dFOXO pathway activity and increasing oxidative stress. Physical exercise protected muscle from accelerated aging induced by high-salt stress through activating muscle NAD+/dSir2/dFOXO pathway and enhancing muscle oxidation resistance. The combination of exercise and muscle dSir2 overexpression had the best protective effect on muscle aging and lifespan in flies.http://www.sciencedirect.com/science/article/pii/S2213453023000551Physical exercisehigh-salt intakemuscle agingNAD+/dSir2 |
| spellingShingle | Dengtai Wen Yiling Chen Xu Tian Wenqi Hou Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila Food Science and Human Wellness Physical exercise high-salt intake muscle aging NAD+/dSir2 |
| title | Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila |
| title_full | Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila |
| title_fullStr | Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila |
| title_full_unstemmed | Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila |
| title_short | Physical exercise improves the premature muscle aging and lifespan reduction induced by high-salt intake and muscle CG2196(salt) overexpression in Drosophila |
| title_sort | physical exercise improves the premature muscle aging and lifespan reduction induced by high salt intake and muscle cg2196 salt overexpression in drosophila |
| topic | Physical exercise high-salt intake muscle aging NAD+/dSir2 |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000551 |
| work_keys_str_mv | AT dengtaiwen physicalexerciseimprovestheprematuremuscleagingandlifespanreductioninducedbyhighsaltintakeandmusclecg2196saltoverexpressionindrosophila AT yilingchen physicalexerciseimprovestheprematuremuscleagingandlifespanreductioninducedbyhighsaltintakeandmusclecg2196saltoverexpressionindrosophila AT xutian physicalexerciseimprovestheprematuremuscleagingandlifespanreductioninducedbyhighsaltintakeandmusclecg2196saltoverexpressionindrosophila AT wenqihou physicalexerciseimprovestheprematuremuscleagingandlifespanreductioninducedbyhighsaltintakeandmusclecg2196saltoverexpressionindrosophila |