A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model
<b>Background</b>: Acute otitis media (AOM) is a common pediatric infection worldwide and is the primary basis for pediatric primary care visits and antibiotic prescriptions in children. Current licensed vaccines have been incompletely ineffective at reducing the global burden of AOM, un...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2076-393X/12/12/1432 |
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| author | Haley Echlin Amy Iverson Abigail McKnight Jason W. Rosch |
| author_facet | Haley Echlin Amy Iverson Abigail McKnight Jason W. Rosch |
| author_sort | Haley Echlin |
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| description | <b>Background</b>: Acute otitis media (AOM) is a common pediatric infection worldwide and is the primary basis for pediatric primary care visits and antibiotic prescriptions in children. Current licensed vaccines have been incompletely ineffective at reducing the global burden of AOM, underscoring a major unmet medical need. The complex etiology of AOM presents additional challenges for vaccine development, as it can stem from multiple bacterial species including <i>Streptococcus pneumoniae</i>, <i>Haemophilus influenzae</i>, and <i>Moraxella catarrhalis</i>. As such, targeting multiple pathogens simultaneously may be required to significantly impact the overall disease burden. <b>Methods</b>: In this study, we aim to overcome this challenge by engineering a live-attenuated vaccine platform based on an attenuated mutant of <i>S. pneumoniae</i> that expresses <i>H. influenzae</i> and <i>M. catarrhalis</i> surface epitopes to induce protective immunity against all three pathogens. <b>Results</b>: The trivalent live-attenuated vaccine conferred significant protection against all three bacterial otopathogens as measured by seroconversion and the development of AOM, with the inclusion of the additional epitopes providing unexpected synergy and enhanced protection against <i>S. pneumoniae</i>. <b>Conclusions</b>: These data demonstrate a novel mechanism of introducing non-native immunogenic antigens into a live-attenuated vaccine platform to engender protection against AOM from multiple pathogenic species. |
| format | Article |
| id | doaj-art-699054bcc03e469fbd5894020aa18e75 |
| institution | OA Journals |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-699054bcc03e469fbd5894020aa18e752025-08-20T02:01:14ZengMDPI AGVaccines2076-393X2024-12-011212143210.3390/vaccines12121432A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine ModelHaley Echlin0Amy Iverson1Abigail McKnight2Jason W. Rosch3Department of Host-Microbe Interactions, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Host-Microbe Interactions, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Host-Microbe Interactions, St. Jude Children’s Research Hospital, Memphis, TN 38105, USADepartment of Host-Microbe Interactions, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA<b>Background</b>: Acute otitis media (AOM) is a common pediatric infection worldwide and is the primary basis for pediatric primary care visits and antibiotic prescriptions in children. Current licensed vaccines have been incompletely ineffective at reducing the global burden of AOM, underscoring a major unmet medical need. The complex etiology of AOM presents additional challenges for vaccine development, as it can stem from multiple bacterial species including <i>Streptococcus pneumoniae</i>, <i>Haemophilus influenzae</i>, and <i>Moraxella catarrhalis</i>. As such, targeting multiple pathogens simultaneously may be required to significantly impact the overall disease burden. <b>Methods</b>: In this study, we aim to overcome this challenge by engineering a live-attenuated vaccine platform based on an attenuated mutant of <i>S. pneumoniae</i> that expresses <i>H. influenzae</i> and <i>M. catarrhalis</i> surface epitopes to induce protective immunity against all three pathogens. <b>Results</b>: The trivalent live-attenuated vaccine conferred significant protection against all three bacterial otopathogens as measured by seroconversion and the development of AOM, with the inclusion of the additional epitopes providing unexpected synergy and enhanced protection against <i>S. pneumoniae</i>. <b>Conclusions</b>: These data demonstrate a novel mechanism of introducing non-native immunogenic antigens into a live-attenuated vaccine platform to engender protection against AOM from multiple pathogenic species.https://www.mdpi.com/2076-393X/12/12/1432acute otitis medialive-attenuated vaccinemultiple otopathogens |
| spellingShingle | Haley Echlin Amy Iverson Abigail McKnight Jason W. Rosch A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model Vaccines acute otitis media live-attenuated vaccine multiple otopathogens |
| title | A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model |
| title_full | A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model |
| title_fullStr | A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model |
| title_full_unstemmed | A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model |
| title_short | A Trivalent Live Vaccine Elicits Cross-Species Protection Against Acute Otitis Media in a Murine Model |
| title_sort | trivalent live vaccine elicits cross species protection against acute otitis media in a murine model |
| topic | acute otitis media live-attenuated vaccine multiple otopathogens |
| url | https://www.mdpi.com/2076-393X/12/12/1432 |
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