Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens
Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) has resulted in significant losses for the poultry industry worldwide. Currently, there is no widely promoted vaccine for NE. In this study, immunoprecipitation (IP) was employed to isolate immunogenic proteins of C. perfring...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010149 |
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| author | Zewei Li Yifei Chen Haiping Xie Quan Li Shifeng Wang Huoying Shi |
| author_facet | Zewei Li Yifei Chen Haiping Xie Quan Li Shifeng Wang Huoying Shi |
| author_sort | Zewei Li |
| collection | DOAJ |
| description | Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) has resulted in significant losses for the poultry industry worldwide. Currently, there is no widely promoted vaccine for NE. In this study, immunoprecipitation (IP) was employed to isolate immunogenic proteins of C. perfringens, and 118 potential candidate antigens were identified through liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS). From these, three candidate antigen proteins were selected based on their predicted antigenicity, hydrophilicity, stability, and transmembrane signalling properties: ArcB (an ornithine aminotransferase), TmpC (a probable membrane lipoprotein), and EntB (a possible enterotoxin). These three proteins were successfully produced in large quantities using Escherichia coli (E. coli), with confirmed good solubility. Both in vitro and in vivo research demonstrated that these antigens possess strong immunogenicity, eliciting robust antigen-specific humoral and cellular immune responses in chickens and mitigating NE symptoms caused by C. perfringens. The candidate antigens identified through immunoproteomics hold potential as subunit vaccines against C. perfringens infection. |
| format | Article |
| id | doaj-art-6989eec3ee004a559d5bae9abb958b98 |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-6989eec3ee004a559d5bae9abb958b982025-08-20T02:39:31ZengElsevierPoultry Science0032-57912024-12-011031210443610.1016/j.psj.2024.104436Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringensZewei Li0Yifei Chen1Haiping Xie2Quan Li3Shifeng Wang4Huoying Shi5College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009 Jiangsu China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009 Jiangsu China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009 Jiangsu China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009 Jiangsu China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, ChinaDepartment of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611-0880, USACollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009 Jiangsu China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University (JIRLAAPS), Yangzhou, China; Corresponding author at: College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, PR China.Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) has resulted in significant losses for the poultry industry worldwide. Currently, there is no widely promoted vaccine for NE. In this study, immunoprecipitation (IP) was employed to isolate immunogenic proteins of C. perfringens, and 118 potential candidate antigens were identified through liquid chromatography-mass spectrometry/mass spectrometry (LC–MS/MS). From these, three candidate antigen proteins were selected based on their predicted antigenicity, hydrophilicity, stability, and transmembrane signalling properties: ArcB (an ornithine aminotransferase), TmpC (a probable membrane lipoprotein), and EntB (a possible enterotoxin). These three proteins were successfully produced in large quantities using Escherichia coli (E. coli), with confirmed good solubility. Both in vitro and in vivo research demonstrated that these antigens possess strong immunogenicity, eliciting robust antigen-specific humoral and cellular immune responses in chickens and mitigating NE symptoms caused by C. perfringens. The candidate antigens identified through immunoproteomics hold potential as subunit vaccines against C. perfringens infection.http://www.sciencedirect.com/science/article/pii/S0032579124010149Necrotic enteritisImmunogenic antigenSubunit vaccineClostridium perfringens |
| spellingShingle | Zewei Li Yifei Chen Haiping Xie Quan Li Shifeng Wang Huoying Shi Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens Poultry Science Necrotic enteritis Immunogenic antigen Subunit vaccine Clostridium perfringens |
| title | Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens |
| title_full | Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens |
| title_fullStr | Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens |
| title_full_unstemmed | Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens |
| title_short | Identification of immunogenic antigens and evaluation of vaccine candidates against Clostridium perfringens |
| title_sort | identification of immunogenic antigens and evaluation of vaccine candidates against clostridium perfringens |
| topic | Necrotic enteritis Immunogenic antigen Subunit vaccine Clostridium perfringens |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010149 |
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