Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin
Objective: Antibiotic resistance is a worldwide health challenge. Close interaction with dogs carrying antibiotic-resistant zoonotic agents poses a risk to human health. The present study aimed to characterize antibiotic-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) isol...
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Network for the Veterinarians of Bangladesh
2025-04-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=248123 |
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| author | Ayaovi Bruno Yaovi Arpita Das Rama N. Behera Prudencio Comlan Sossa-Minou Vinod Singh Bisht Mónica Yadav Ayushi Kapoor Francois Dossa Paulin Azokpota Souaïbou Farougou Lamine Baba-Moussa Kiran Ambatipudi Philippe Sessou |
| author_facet | Ayaovi Bruno Yaovi Arpita Das Rama N. Behera Prudencio Comlan Sossa-Minou Vinod Singh Bisht Mónica Yadav Ayushi Kapoor Francois Dossa Paulin Azokpota Souaïbou Farougou Lamine Baba-Moussa Kiran Ambatipudi Philippe Sessou |
| author_sort | Ayaovi Bruno Yaovi |
| collection | DOAJ |
| description | Objective: Antibiotic resistance is a worldwide health challenge. Close interaction with dogs carrying antibiotic-resistant zoonotic agents poses a risk to human health. The present study aimed to characterize antibiotic-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) isolated from dogs in Southern Benin.
Materials and methods: A total of 336 swabs (112 buccal, 112 nasal, and 112 rectal) from 112 dogs living in the communes of Abomey-Calavi and Cotonou were analyzed for E. coli and S. aureus presence. Bacterial isolates were tested for antibiotic (penicillins, tetracyclines, aminoglycosides, cephalosporins, sulfonamides, and macrolides) susceptibility using the disc diffusion method, and antibiotic-resistant strains were characterized by the polymerase chain reaction method.
Results: A 41.07% and 20.53% of dogs harbored E. coli and S. aureus, respectively. Escherichia coli and S. aureus isolates showed resistance to penicillin (100% and 81.48%), tetracycline (44.64% and 59.26%), and other antimicrobials tested. Escherichia coli isolates harbored resistance genes blaTEM (63.46%), tetA (62.50%), and strA-strB (55.56%). tetK (100%), tetM (100%), and blaZ (82.61%) were present in S. aureus isolates. Escherichia coli strains harbored virulence genes fimH (61.54%), kpsMTII (26.92%), fyuA (19.23%), and eae (1.92%), whereas 20.83% of S. aureus strains harbored pvl and fnbA.
Conclusion: The results of the current study reveal the urgent need for stricter controls on antibiotic use. Implementing guidelines, responsible prescribing, and increasing public awareness are crucial steps to address this problem. [J Adv Vet Anim Res 2025; 12(2.000): 396-419] |
| format | Article |
| id | doaj-art-69860d61247b46c3afcdb07b7d7866cf |
| institution | Kabale University |
| issn | 2311-7710 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj-art-69860d61247b46c3afcdb07b7d7866cf2025-08-20T03:58:35ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102025-04-0112239641910.5455/javar.2025.l907248123Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern BeninAyaovi Bruno Yaovi0Arpita Das1Rama N. Behera2Prudencio Comlan Sossa-Minou3Vinod Singh Bisht4Mónica Yadav5Ayushi Kapoor6Francois Dossa7Paulin Azokpota8Souaïbou Farougou9Lamine Baba-Moussa10Kiran Ambatipudi11Philippe Sessou12Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin Laboratory of Food Science and Technology, University of Abomey-Calavi, Cotonou, Benin Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin Laboratory of Biochemistry and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India Research Unit on Communicable Diseases, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin.Objective: Antibiotic resistance is a worldwide health challenge. Close interaction with dogs carrying antibiotic-resistant zoonotic agents poses a risk to human health. The present study aimed to characterize antibiotic-resistant Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) isolated from dogs in Southern Benin. Materials and methods: A total of 336 swabs (112 buccal, 112 nasal, and 112 rectal) from 112 dogs living in the communes of Abomey-Calavi and Cotonou were analyzed for E. coli and S. aureus presence. Bacterial isolates were tested for antibiotic (penicillins, tetracyclines, aminoglycosides, cephalosporins, sulfonamides, and macrolides) susceptibility using the disc diffusion method, and antibiotic-resistant strains were characterized by the polymerase chain reaction method. Results: A 41.07% and 20.53% of dogs harbored E. coli and S. aureus, respectively. Escherichia coli and S. aureus isolates showed resistance to penicillin (100% and 81.48%), tetracycline (44.64% and 59.26%), and other antimicrobials tested. Escherichia coli isolates harbored resistance genes blaTEM (63.46%), tetA (62.50%), and strA-strB (55.56%). tetK (100%), tetM (100%), and blaZ (82.61%) were present in S. aureus isolates. Escherichia coli strains harbored virulence genes fimH (61.54%), kpsMTII (26.92%), fyuA (19.23%), and eae (1.92%), whereas 20.83% of S. aureus strains harbored pvl and fnbA. Conclusion: The results of the current study reveal the urgent need for stricter controls on antibiotic use. Implementing guidelines, responsible prescribing, and increasing public awareness are crucial steps to address this problem. [J Adv Vet Anim Res 2025; 12(2.000): 396-419]http://www.ejmanager.com/fulltextpdf.php?mno=248123prevalence; escherichia coli; staphylococcus aureus; dogs; benin |
| spellingShingle | Ayaovi Bruno Yaovi Arpita Das Rama N. Behera Prudencio Comlan Sossa-Minou Vinod Singh Bisht Mónica Yadav Ayushi Kapoor Francois Dossa Paulin Azokpota Souaïbou Farougou Lamine Baba-Moussa Kiran Ambatipudi Philippe Sessou Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin Journal of Advanced Veterinary and Animal Research prevalence; escherichia coli; staphylococcus aureus; dogs; benin |
| title | Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin |
| title_full | Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin |
| title_fullStr | Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin |
| title_full_unstemmed | Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin |
| title_short | Molecular identification of antibiotic-resistant and virulent Escherichia coli and Staphylococcus aureus isolated from dogs in Southern Benin |
| title_sort | molecular identification of antibiotic resistant and virulent escherichia coli and staphylococcus aureus isolated from dogs in southern benin |
| topic | prevalence; escherichia coli; staphylococcus aureus; dogs; benin |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=248123 |
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