Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation.
An increasing number of investigations including human studies demonstrate that pharmacological ischaemic preconditioning is a viable way to protect the heart from myocardial ischaemia/reperfusion (I/R) injury. This study investigated the role of hydroxychloroquine (HCQ) in the heart during I/R inju...
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| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0143771 |
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| author | Lauren Bourke James McCormick Valerie Taylor Charis Pericleous Benoit Blanchet Nathalie Costedoat-Chalumeau Daniel Stuckey Mark F Lythgoe Anastasis Stephanou Yiannis Ioannou |
| author_facet | Lauren Bourke James McCormick Valerie Taylor Charis Pericleous Benoit Blanchet Nathalie Costedoat-Chalumeau Daniel Stuckey Mark F Lythgoe Anastasis Stephanou Yiannis Ioannou |
| author_sort | Lauren Bourke |
| collection | DOAJ |
| description | An increasing number of investigations including human studies demonstrate that pharmacological ischaemic preconditioning is a viable way to protect the heart from myocardial ischaemia/reperfusion (I/R) injury. This study investigated the role of hydroxychloroquine (HCQ) in the heart during I/R injury. In vitro and in vivo models of myocardial I/R injury were used to assess the effects of HCQ. It was found that HCQ was protective in neonatal rat cardiomyocytes through inhibition of apoptosis, measured by TUNEL and cleaved caspase-3. This protection in vitro was mediated through enhancement of ERK1/2 phosphorylation mediated by HCQ in a dose-dependent fashion. A decrease in infarct size was observed in an in vivo model of myocardial I/R injury in HCQ treated animals and furthermore this protection was blocked in the presence of the ERK1/2 inhibitor U0126. For the first time, we have shown that HCQ promotes a preconditioning like protection in an in vivo simulated rat myocardial I/R injury model. Moreover, it was shown that HCQ is protective via enhanced phosphorylation of the pro-survival kinase ERK1/2. |
| format | Article |
| id | doaj-art-6984a75f58f8453083e9a4ae86d1b1d7 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-6984a75f58f8453083e9a4ae86d1b1d72025-08-20T03:10:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014377110.1371/journal.pone.0143771Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation.Lauren BourkeJames McCormickValerie TaylorCharis PericleousBenoit BlanchetNathalie Costedoat-ChalumeauDaniel StuckeyMark F LythgoeAnastasis StephanouYiannis IoannouAn increasing number of investigations including human studies demonstrate that pharmacological ischaemic preconditioning is a viable way to protect the heart from myocardial ischaemia/reperfusion (I/R) injury. This study investigated the role of hydroxychloroquine (HCQ) in the heart during I/R injury. In vitro and in vivo models of myocardial I/R injury were used to assess the effects of HCQ. It was found that HCQ was protective in neonatal rat cardiomyocytes through inhibition of apoptosis, measured by TUNEL and cleaved caspase-3. This protection in vitro was mediated through enhancement of ERK1/2 phosphorylation mediated by HCQ in a dose-dependent fashion. A decrease in infarct size was observed in an in vivo model of myocardial I/R injury in HCQ treated animals and furthermore this protection was blocked in the presence of the ERK1/2 inhibitor U0126. For the first time, we have shown that HCQ promotes a preconditioning like protection in an in vivo simulated rat myocardial I/R injury model. Moreover, it was shown that HCQ is protective via enhanced phosphorylation of the pro-survival kinase ERK1/2.https://doi.org/10.1371/journal.pone.0143771 |
| spellingShingle | Lauren Bourke James McCormick Valerie Taylor Charis Pericleous Benoit Blanchet Nathalie Costedoat-Chalumeau Daniel Stuckey Mark F Lythgoe Anastasis Stephanou Yiannis Ioannou Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. PLoS ONE |
| title | Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. |
| title_full | Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. |
| title_fullStr | Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. |
| title_full_unstemmed | Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. |
| title_short | Hydroxychloroquine Protects against Cardiac Ischaemia/Reperfusion Injury In Vivo via Enhancement of ERK1/2 Phosphorylation. |
| title_sort | hydroxychloroquine protects against cardiac ischaemia reperfusion injury in vivo via enhancement of erk1 2 phosphorylation |
| url | https://doi.org/10.1371/journal.pone.0143771 |
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