On‐chip label‐free impedance‐based detection of antibiotic permeation
Abstract Biosensors are analytical tools used for the analysis of biomaterial samples and provide an understanding about the biocomposition, structure, and function of biomolecules and/or biomechanisms by converting the biological response into an electrical and/or optical signal. In particular, wit...
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| Format: | Article |
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Wiley
2021-02-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12019 |
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| author | Jaspreet Kaur Hamed Ghorbanpoor Yasin Öztürk Özge Kaygusuz Hüseyin Avcı Cihan Darcan Levent Trabzon Fatma D. Güzel |
| author_facet | Jaspreet Kaur Hamed Ghorbanpoor Yasin Öztürk Özge Kaygusuz Hüseyin Avcı Cihan Darcan Levent Trabzon Fatma D. Güzel |
| author_sort | Jaspreet Kaur |
| collection | DOAJ |
| description | Abstract Biosensors are analytical tools used for the analysis of biomaterial samples and provide an understanding about the biocomposition, structure, and function of biomolecules and/or biomechanisms by converting the biological response into an electrical and/or optical signal. In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. Herein, for the rapid and label‐free detection of antibiotic permeation across a membrane, a microelectrode integrated microfluidic device is presented. The integrated chip consists of polydimethylsiloxane based microfluidic channels bonded onto microelectrodes on‐glass and enables us to recognize the antibiotic permeation across a membrane into the model membranes based on electrical impedance measurement, while also allowing optical monitoring. Impedance testing is label free and therefore allows the detection of both fluorescent and non‐fluorescent antibiotics. As a model membrane, Giant Unilamellar Vesicles (GUVs) are used and impedance measurements were performed by a precision inductance, capacitance, and resistance metre. The measured signal recorded from the device was used to determine the change in concentration inside and outside of the GUVs. We have found that permeation of antibiotic molecules can be easily monitored over time using the proposed integrated device. The results also show a clear difference between bilayer permeation that occurs through the lipidic bilayer and porin‐mediated permeation through the porin channels inserted in the lipid bilayer. |
| format | Article |
| id | doaj-art-69849fa1ec574160ad15803a31760a5d |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-69849fa1ec574160ad15803a31760a5d2025-02-03T01:29:38ZengWileyIET Nanobiotechnology1751-87411751-875X2021-02-0115110010610.1049/nbt2.12019On‐chip label‐free impedance‐based detection of antibiotic permeationJaspreet Kaur0Hamed Ghorbanpoor1Yasin Öztürk2Özge Kaygusuz3Hüseyin Avcı4Cihan Darcan5Levent Trabzon6Fatma D. Güzel7Department of Electrical and Electronic Engineering Yıldırım Beyazıt University Ankara TurkeyDepartment of Biomedical Engineering Yıldırım Beyazıt University Ankara TurkeyDepartment of Material Engineering Yıldırım Beyazıt University Ankara TurkeyBiotechnology Application and Research Center Bilecik Şeyh Edebali University Bilecik TurkeyMetallurgical and Materials Engineering Department Eskisehir Osmangazi University Eskisehir TurkeyBiotechnology Application and Research Center Bilecik Şeyh Edebali University Bilecik TurkeyDepartment of Mechanical Engineering Istanbul Technical University Istanbul TurkeyDepartment of Biomedical Engineering Yıldırım Beyazıt University Ankara TurkeyAbstract Biosensors are analytical tools used for the analysis of biomaterial samples and provide an understanding about the biocomposition, structure, and function of biomolecules and/or biomechanisms by converting the biological response into an electrical and/or optical signal. In particular, with the rise in antibiotic resistance amongst pathogenic bacteria, the study of antibiotic activity and transport across cell membranes in the field of biosensors has been gaining widespread importance. Herein, for the rapid and label‐free detection of antibiotic permeation across a membrane, a microelectrode integrated microfluidic device is presented. The integrated chip consists of polydimethylsiloxane based microfluidic channels bonded onto microelectrodes on‐glass and enables us to recognize the antibiotic permeation across a membrane into the model membranes based on electrical impedance measurement, while also allowing optical monitoring. Impedance testing is label free and therefore allows the detection of both fluorescent and non‐fluorescent antibiotics. As a model membrane, Giant Unilamellar Vesicles (GUVs) are used and impedance measurements were performed by a precision inductance, capacitance, and resistance metre. The measured signal recorded from the device was used to determine the change in concentration inside and outside of the GUVs. We have found that permeation of antibiotic molecules can be easily monitored over time using the proposed integrated device. The results also show a clear difference between bilayer permeation that occurs through the lipidic bilayer and porin‐mediated permeation through the porin channels inserted in the lipid bilayer.https://doi.org/10.1049/nbt2.12019biochemistrybioelectric phenomenabiological techniquesbiomembranesbioMEMScellular biophysics |
| spellingShingle | Jaspreet Kaur Hamed Ghorbanpoor Yasin Öztürk Özge Kaygusuz Hüseyin Avcı Cihan Darcan Levent Trabzon Fatma D. Güzel On‐chip label‐free impedance‐based detection of antibiotic permeation IET Nanobiotechnology biochemistry bioelectric phenomena biological techniques biomembranes bioMEMS cellular biophysics |
| title | On‐chip label‐free impedance‐based detection of antibiotic permeation |
| title_full | On‐chip label‐free impedance‐based detection of antibiotic permeation |
| title_fullStr | On‐chip label‐free impedance‐based detection of antibiotic permeation |
| title_full_unstemmed | On‐chip label‐free impedance‐based detection of antibiotic permeation |
| title_short | On‐chip label‐free impedance‐based detection of antibiotic permeation |
| title_sort | on chip label free impedance based detection of antibiotic permeation |
| topic | biochemistry bioelectric phenomena biological techniques biomembranes bioMEMS cellular biophysics |
| url | https://doi.org/10.1049/nbt2.12019 |
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