Imaging the enteric nervous system
The enteric nervous system (ENS) has garnered increasing scientific interest due to its pivotal role in digestive processes and its involvement in various gastrointestinal and central nervous system (CNS) disorders, including Crohn’s disease, Parkinson’s disease, and autism. Despite its significance...
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2025.1532900/full |
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| author | Doriane Hazart Doriane Hazart Marwa Moulzir Brigitte Delhomme Martin Oheim Clément Ricard |
| author_facet | Doriane Hazart Doriane Hazart Marwa Moulzir Brigitte Delhomme Martin Oheim Clément Ricard |
| author_sort | Doriane Hazart |
| collection | DOAJ |
| description | The enteric nervous system (ENS) has garnered increasing scientific interest due to its pivotal role in digestive processes and its involvement in various gastrointestinal and central nervous system (CNS) disorders, including Crohn’s disease, Parkinson’s disease, and autism. Despite its significance, the ENS remains relatively underexplored by neurobiologists, primarily because its structure and function are less understood compared to the CNS. This review examines both pioneering methodologies that initially revealed the intricate layered structure of the ENS and recent advancements in studying its three-dimensional (3-D) organization, both in fixed samples and at a functional level, ex-vivo or in-vivo. Traditionally, imaging the ENS relied on histological techniques involving sequential tissue sectioning, staining, and microscopic imaging of single sections. However, this method has limitations representing the full complexity of the ENS’s 3-D meshwork, which led to the development of more intact preparations, such as whole-mount preparation, as well as the use of volume imaging techniques. Advancements in 3-D imaging, particularly methods like spinning-disk confocal, 2-photon, and light-sheet microscopies, combined with tissue-clearing techniques, have revolutionized our understanding of the ENS’s fine structure. These approaches offer detailed views of its cellular architecture, including interactions among various cell types, blood vessels, and lymphatic vessels. They have also enhanced our comprehension of ENS-related pathologies, such as inflammatory bowel disease, Hirschsprung’s disease (HSCR), and the ENS’s involvement in neurodegenerative disorders like Parkinson’s (PD) and Alzheimer’s diseases (AD). More recently, 2-photon or confocal in-vivo imaging, combined with transgenic approaches for calcium imaging, or confocal laser endomicroscopy, have opened new avenues for functional studies of the ENS. These methods enable real-time observation of enteric neuronal and glial activity and their interactions. While routinely used in CNS studies, their application to understanding local circuits and signals in the ENS is relatively recent and presents unique challenges, such as accommodating peristaltic movements. Advancements in 3-D in-vivo functional imaging are expected to significantly deepen our understanding of the ENS and its roles in gastrointestinal and neurological diseases, potentially leading to improved diagnostic and therapeutic strategies. |
| format | Article |
| id | doaj-art-697f1028059043cbbfb4c8e14fcb638f |
| institution | DOAJ |
| issn | 1662-5129 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj-art-697f1028059043cbbfb4c8e14fcb638f2025-08-20T02:58:53ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292025-03-011910.3389/fnana.2025.15329001532900Imaging the enteric nervous systemDoriane Hazart0Doriane Hazart1Marwa Moulzir2Brigitte Delhomme3Martin Oheim4Clément Ricard5Université Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, FranceDoctoral School Brain, Cognition and Behaviour – ED3C - ED 158, Paris, FranceUniversité Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, FranceUniversité Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, FranceUniversité Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, FranceUniversité Paris Cité, CNRS, Saints-Pères Paris Institute for the Neurosciences, Paris, FranceThe enteric nervous system (ENS) has garnered increasing scientific interest due to its pivotal role in digestive processes and its involvement in various gastrointestinal and central nervous system (CNS) disorders, including Crohn’s disease, Parkinson’s disease, and autism. Despite its significance, the ENS remains relatively underexplored by neurobiologists, primarily because its structure and function are less understood compared to the CNS. This review examines both pioneering methodologies that initially revealed the intricate layered structure of the ENS and recent advancements in studying its three-dimensional (3-D) organization, both in fixed samples and at a functional level, ex-vivo or in-vivo. Traditionally, imaging the ENS relied on histological techniques involving sequential tissue sectioning, staining, and microscopic imaging of single sections. However, this method has limitations representing the full complexity of the ENS’s 3-D meshwork, which led to the development of more intact preparations, such as whole-mount preparation, as well as the use of volume imaging techniques. Advancements in 3-D imaging, particularly methods like spinning-disk confocal, 2-photon, and light-sheet microscopies, combined with tissue-clearing techniques, have revolutionized our understanding of the ENS’s fine structure. These approaches offer detailed views of its cellular architecture, including interactions among various cell types, blood vessels, and lymphatic vessels. They have also enhanced our comprehension of ENS-related pathologies, such as inflammatory bowel disease, Hirschsprung’s disease (HSCR), and the ENS’s involvement in neurodegenerative disorders like Parkinson’s (PD) and Alzheimer’s diseases (AD). More recently, 2-photon or confocal in-vivo imaging, combined with transgenic approaches for calcium imaging, or confocal laser endomicroscopy, have opened new avenues for functional studies of the ENS. These methods enable real-time observation of enteric neuronal and glial activity and their interactions. While routinely used in CNS studies, their application to understanding local circuits and signals in the ENS is relatively recent and presents unique challenges, such as accommodating peristaltic movements. Advancements in 3-D in-vivo functional imaging are expected to significantly deepen our understanding of the ENS and its roles in gastrointestinal and neurological diseases, potentially leading to improved diagnostic and therapeutic strategies.https://www.frontiersin.org/articles/10.3389/fnana.2025.1532900/full3-D imaginghistological methodwhole-mount preparation techniquein-vivo imagingclearing methodintestine |
| spellingShingle | Doriane Hazart Doriane Hazart Marwa Moulzir Brigitte Delhomme Martin Oheim Clément Ricard Imaging the enteric nervous system Frontiers in Neuroanatomy 3-D imaging histological method whole-mount preparation technique in-vivo imaging clearing method intestine |
| title | Imaging the enteric nervous system |
| title_full | Imaging the enteric nervous system |
| title_fullStr | Imaging the enteric nervous system |
| title_full_unstemmed | Imaging the enteric nervous system |
| title_short | Imaging the enteric nervous system |
| title_sort | imaging the enteric nervous system |
| topic | 3-D imaging histological method whole-mount preparation technique in-vivo imaging clearing method intestine |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2025.1532900/full |
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