Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics
Proteins represent a significant portion of the global therapeutics market, surpassing hundreds of billions of dollars annually. Among the various post-translational modifications, glycosylation plays a crucial role in influencing protein structure, stability, and function. This modification is espe...
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MDPI AG
2025-03-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/7/2064 |
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| author | Preety Ahuja Manpreet Singh Sanjeev Kumar Ujjain |
| author_facet | Preety Ahuja Manpreet Singh Sanjeev Kumar Ujjain |
| author_sort | Preety Ahuja |
| collection | DOAJ |
| description | Proteins represent a significant portion of the global therapeutics market, surpassing hundreds of billions of dollars annually. Among the various post-translational modifications, glycosylation plays a crucial role in influencing protein structure, stability, and function. This modification is especially important in biotherapeutics, where the precise characterization of glycans is vital for ensuring product efficacy and safety. Although mass spectrometry-based techniques have become essential tools for glycomic analysis due to their high sensitivity and resolution, their complexity and lengthy processing times limit their practical application. In contrast, electrochemical methods provide a rapid, cost-effective, and sensitive alternative for glycosylation assessment, enabling the real-time analysis of glycan structures on biotherapeutic proteins. These electrochemical techniques, often used in conjunction with complementary methods, offer valuable insights into the glycosylation profiles of both isolated glycoproteins and intact cells. This review examines the latest advancements in electrochemical biosensors for glycosylation analysis, highlighting their potential in enhancing the characterization of biotherapeutics and advancing the field of precision medicine. |
| format | Article |
| id | doaj-art-696a8c2039d640e1a819e36be8a5cf35 |
| institution | DOAJ |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-696a8c2039d640e1a819e36be8a5cf352025-08-20T03:03:25ZengMDPI AGSensors1424-82202025-03-01257206410.3390/s25072064Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of BiotherapeuticsPreety Ahuja0Manpreet Singh1Sanjeev Kumar Ujjain2Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USADepartment of Mechanical Engineering, College of Engineering and Information Technology, University of Maryland Baltimore County, Baltimore, MD 21250, USADepartment of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USAProteins represent a significant portion of the global therapeutics market, surpassing hundreds of billions of dollars annually. Among the various post-translational modifications, glycosylation plays a crucial role in influencing protein structure, stability, and function. This modification is especially important in biotherapeutics, where the precise characterization of glycans is vital for ensuring product efficacy and safety. Although mass spectrometry-based techniques have become essential tools for glycomic analysis due to their high sensitivity and resolution, their complexity and lengthy processing times limit their practical application. In contrast, electrochemical methods provide a rapid, cost-effective, and sensitive alternative for glycosylation assessment, enabling the real-time analysis of glycan structures on biotherapeutic proteins. These electrochemical techniques, often used in conjunction with complementary methods, offer valuable insights into the glycosylation profiles of both isolated glycoproteins and intact cells. This review examines the latest advancements in electrochemical biosensors for glycosylation analysis, highlighting their potential in enhancing the characterization of biotherapeutics and advancing the field of precision medicine.https://www.mdpi.com/1424-8220/25/7/2064electrochemical biosensorsglycosylation assessmentbiotherapeuticsquality controlsurface modification |
| spellingShingle | Preety Ahuja Manpreet Singh Sanjeev Kumar Ujjain Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics Sensors electrochemical biosensors glycosylation assessment biotherapeutics quality control surface modification |
| title | Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics |
| title_full | Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics |
| title_fullStr | Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics |
| title_full_unstemmed | Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics |
| title_short | Advancements in Electrochemical Biosensors for Comprehensive Glycosylation Assessment of Biotherapeutics |
| title_sort | advancements in electrochemical biosensors for comprehensive glycosylation assessment of biotherapeutics |
| topic | electrochemical biosensors glycosylation assessment biotherapeutics quality control surface modification |
| url | https://www.mdpi.com/1424-8220/25/7/2064 |
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