Advances in the molecular diagnostic methods for circular RNA
Circular RNA (circRNA) is a unique type of non-coding RNA, which is produced by back splicing of mRNA to form the covalent junction without 3′-5′ polarity and poly(A) tail. With the development of detection technology, a large number of circRNAs have been identified in organisms, and their biologica...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Biomedical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950435X24000374 |
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| author | Zequan Ye Ke Wang Jianhua Zhou Jiasi Wang |
| author_facet | Zequan Ye Ke Wang Jianhua Zhou Jiasi Wang |
| author_sort | Zequan Ye |
| collection | DOAJ |
| description | Circular RNA (circRNA) is a unique type of non-coding RNA, which is produced by back splicing of mRNA to form the covalent junction without 3′-5′ polarity and poly(A) tail. With the development of detection technology, a large number of circRNAs have been identified in organisms, and their biological functions and mechanisms have been uncovered gradually. In particular, circRNAs are abnormally expressed and play a critical role in many diseases, including gastric cancer, hepatocellular carcinoma, breast cancer, etc., showing great potential as disease biomarkers. At present, the main methods for detecting circRNA include RNA sequencing, RT-PCR, Northern blotting, etc., which have limited sensitivity and specificity. Recently, some new methods, such as isothermal amplification and digital droplet platform, have emerged to improve the sensitivity and quantitative accuracy for circRNA detection. In this review, we summarized the traditional and emerging methods for circRNA detection as well as the advantages and limitations, and look forward to future research directions. |
| format | Article |
| id | doaj-art-696a4ace321f405381d8eb245930ed12 |
| institution | Kabale University |
| issn | 2950-435X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Biomedical Analysis |
| spelling | doaj-art-696a4ace321f405381d8eb245930ed122025-08-20T03:42:43ZengKeAi Communications Co., Ltd.Biomedical Analysis2950-435X2024-12-011427127910.1016/j.bioana.2024.11.002Advances in the molecular diagnostic methods for circular RNAZequan Ye0Ke Wang1Jianhua Zhou2Jiasi Wang3Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaGuangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China; School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China; School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510275, China; Corresponding author at: Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.Circular RNA (circRNA) is a unique type of non-coding RNA, which is produced by back splicing of mRNA to form the covalent junction without 3′-5′ polarity and poly(A) tail. With the development of detection technology, a large number of circRNAs have been identified in organisms, and their biological functions and mechanisms have been uncovered gradually. In particular, circRNAs are abnormally expressed and play a critical role in many diseases, including gastric cancer, hepatocellular carcinoma, breast cancer, etc., showing great potential as disease biomarkers. At present, the main methods for detecting circRNA include RNA sequencing, RT-PCR, Northern blotting, etc., which have limited sensitivity and specificity. Recently, some new methods, such as isothermal amplification and digital droplet platform, have emerged to improve the sensitivity and quantitative accuracy for circRNA detection. In this review, we summarized the traditional and emerging methods for circRNA detection as well as the advantages and limitations, and look forward to future research directions.http://www.sciencedirect.com/science/article/pii/S2950435X24000374Circular RNAMolecular diagnostic methodsIsothermal amplificationDigital assayCRISPR-Cas |
| spellingShingle | Zequan Ye Ke Wang Jianhua Zhou Jiasi Wang Advances in the molecular diagnostic methods for circular RNA Biomedical Analysis Circular RNA Molecular diagnostic methods Isothermal amplification Digital assay CRISPR-Cas |
| title | Advances in the molecular diagnostic methods for circular RNA |
| title_full | Advances in the molecular diagnostic methods for circular RNA |
| title_fullStr | Advances in the molecular diagnostic methods for circular RNA |
| title_full_unstemmed | Advances in the molecular diagnostic methods for circular RNA |
| title_short | Advances in the molecular diagnostic methods for circular RNA |
| title_sort | advances in the molecular diagnostic methods for circular rna |
| topic | Circular RNA Molecular diagnostic methods Isothermal amplification Digital assay CRISPR-Cas |
| url | http://www.sciencedirect.com/science/article/pii/S2950435X24000374 |
| work_keys_str_mv | AT zequanye advancesinthemoleculardiagnosticmethodsforcircularrna AT kewang advancesinthemoleculardiagnosticmethodsforcircularrna AT jianhuazhou advancesinthemoleculardiagnosticmethodsforcircularrna AT jiasiwang advancesinthemoleculardiagnosticmethodsforcircularrna |