Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis
Abstract Background and aims Recent studies have indicated possible links between the microbiota and the fetal heart, while the relevant mechanism is still unknown. This study is aims to investigate whether analyzing the microbiota and metabolic profiles of amniotic fluid collected from pregnant wom...
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BMC
2025-02-01
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| Series: | BMC Pregnancy and Childbirth |
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| Online Access: | https://doi.org/10.1186/s12884-025-07218-7 |
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| author | Wenli Xu Lu Li Hong Kang Meixian Wang Yanqun Liu Guicun Wang Ping Yu Juan Liang Zhen Liu |
| author_facet | Wenli Xu Lu Li Hong Kang Meixian Wang Yanqun Liu Guicun Wang Ping Yu Juan Liang Zhen Liu |
| author_sort | Wenli Xu |
| collection | DOAJ |
| description | Abstract Background and aims Recent studies have indicated possible links between the microbiota and the fetal heart, while the relevant mechanism is still unknown. This study is aims to investigate whether analyzing the microbiota and metabolic profiles of amniotic fluid collected from pregnant women whose fetuses with or without non-syndromic congenital heart defects (CHDs), during the second and third trimester of pregnancy, could offer valuable insights into CHDs. Methods and results A case-control study was conducted with 17 cases diagnosed with non-syndromic CHDs (CHDs group) and 34 controls without congenital anomalies (control group) at a ratio of 1:2. The 16 S rDNA gene sequencing and metabolomics methods were employed to assess 51 amniotic fluid samples. The amniotic fluid microbiome from the CHDs group exhibited significantly higher Shannon and Simpson indices compared to the control group. At the genus level, 240 bacterial taxa were substantially enriched in the two groups, with 93 of those taxa being highly enriched in the case group. Compared to the control group, the case group exhibited 177 metabolites that were significantly increased and 480 metabolites that were down-regulated. The differential metabolites were primarily enriched in the steroid hormone biosynthesis, bile secretion and ovarian steroidogenesis, according to KEGG analysis. The observed variations in nine metabolites could attributed to fifty-eight distinct bacterial taxa. The nine differential metabolites were mainly associated with pathways involving steroid hormone biosynthesis, bile secretion, glycolysis, tricarboxylic acid (TCA) cycle, NADPH metabolism, and acyl transfer pathways. Conclusion The CHDs group has disturbed amniotic fluid microbiota and metabolites, and more research was required to elucidate the mechanism. |
| format | Article |
| id | doaj-art-69634fb6a980413caf6334aa7bbcd327 |
| institution | Kabale University |
| issn | 1471-2393 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | BMC Pregnancy and Childbirth |
| spelling | doaj-art-69634fb6a980413caf6334aa7bbcd3272025-02-09T12:59:04ZengBMCBMC Pregnancy and Childbirth1471-23932025-02-012511910.1186/s12884-025-07218-7Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysisWenli Xu0Lu Li1Hong Kang2Meixian Wang3Yanqun Liu4Guicun Wang5Ping Yu6Juan Liang7Zhen Liu8National Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityDepartment of Obstetrics and Gynecology, Renshou County Maternity and Child HospitalDepartment of Obstetrics and Gynecology, Huize County Maternity and Child HospitalNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityNational Center for Birth Defect Monitoring, Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second University Hospital, Sichuan UniversityAbstract Background and aims Recent studies have indicated possible links between the microbiota and the fetal heart, while the relevant mechanism is still unknown. This study is aims to investigate whether analyzing the microbiota and metabolic profiles of amniotic fluid collected from pregnant women whose fetuses with or without non-syndromic congenital heart defects (CHDs), during the second and third trimester of pregnancy, could offer valuable insights into CHDs. Methods and results A case-control study was conducted with 17 cases diagnosed with non-syndromic CHDs (CHDs group) and 34 controls without congenital anomalies (control group) at a ratio of 1:2. The 16 S rDNA gene sequencing and metabolomics methods were employed to assess 51 amniotic fluid samples. The amniotic fluid microbiome from the CHDs group exhibited significantly higher Shannon and Simpson indices compared to the control group. At the genus level, 240 bacterial taxa were substantially enriched in the two groups, with 93 of those taxa being highly enriched in the case group. Compared to the control group, the case group exhibited 177 metabolites that were significantly increased and 480 metabolites that were down-regulated. The differential metabolites were primarily enriched in the steroid hormone biosynthesis, bile secretion and ovarian steroidogenesis, according to KEGG analysis. The observed variations in nine metabolites could attributed to fifty-eight distinct bacterial taxa. The nine differential metabolites were mainly associated with pathways involving steroid hormone biosynthesis, bile secretion, glycolysis, tricarboxylic acid (TCA) cycle, NADPH metabolism, and acyl transfer pathways. Conclusion The CHDs group has disturbed amniotic fluid microbiota and metabolites, and more research was required to elucidate the mechanism.https://doi.org/10.1186/s12884-025-07218-7Congenital heart defectsMicrobiotaMetaboliteBirth developmentMultiomics analysis |
| spellingShingle | Wenli Xu Lu Li Hong Kang Meixian Wang Yanqun Liu Guicun Wang Ping Yu Juan Liang Zhen Liu Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis BMC Pregnancy and Childbirth Congenital heart defects Microbiota Metabolite Birth development Multiomics analysis |
| title | Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis |
| title_full | Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis |
| title_fullStr | Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis |
| title_full_unstemmed | Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis |
| title_short | Amniotic fluid microbiota and metabolism with non-syndromic congenital heart defects: a multi-omics analysis |
| title_sort | amniotic fluid microbiota and metabolism with non syndromic congenital heart defects a multi omics analysis |
| topic | Congenital heart defects Microbiota Metabolite Birth development Multiomics analysis |
| url | https://doi.org/10.1186/s12884-025-07218-7 |
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