The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells.
<h4>Background</h4>IgE antibodies play a paramount role in the pathogenesis of various intestinal disorders. To gain insights in IgE-mediated pathophysiology of the gut, we investigated the expression of the high affinity IgE receptor Fc epsilonRI in human intestinal epithelium.<h4>...
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Public Library of Science (PLoS)
2010-02-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009023&type=printable |
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| author | Eva Untersmayr Giovanna Bises Philipp Starkl Charles L Bevins Otto Scheiner George Boltz-Nitulescu Fritz Wrba Erika Jensen-Jarolim |
| author_facet | Eva Untersmayr Giovanna Bises Philipp Starkl Charles L Bevins Otto Scheiner George Boltz-Nitulescu Fritz Wrba Erika Jensen-Jarolim |
| author_sort | Eva Untersmayr |
| collection | DOAJ |
| description | <h4>Background</h4>IgE antibodies play a paramount role in the pathogenesis of various intestinal disorders. To gain insights in IgE-mediated pathophysiology of the gut, we investigated the expression of the high affinity IgE receptor Fc epsilonRI in human intestinal epithelium.<h4>Methodology/principal findings</h4>Fc epsilonRI alpha-chain, as detected by immunohistochemistry, was positive in epithelial cells for eight of eleven (8/11) specimens from colon cancer patients and 5/11 patients with inflammation of the enteric mucosa. The Fc epsilonRIalpha positive epithelial cells co-expressed Fc epsilonRIgamma, whereas with one exception, none of the samples was positive for the beta-chain in the epithelial layer. The functionality of Fc epsilonRI was confirmed in situ by human IgE binding. In experiments with human intestinal tumor cell lines, subconfluent Caco-2/TC7 and HCT-8 cells were found to express the alpha- and gamma-chains of Fc epsilonRI and to bind IgE, whereas confluent cells were negative for gamma-chains.<h4>Conclusions/significance</h4>Our data provide the first evidence that the components of a functional Fc epsilonRI are in vitro expressed by the human intestinal epithelial cells depending on differentiation and, more importantly, in situ in epithelia of patients with colon cancer or gastrointestinal inflammations. Thus, a contribution of Fc epsilonRI either to immunosurveillance or pathophysiology of the intestinal epithelium is suggested. |
| format | Article |
| id | doaj-art-6962f6a1d13c4eee83bdf2eeb91e6769 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2010-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-6962f6a1d13c4eee83bdf2eeb91e67692025-08-20T03:07:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-02-0152e902310.1371/journal.pone.0009023The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells.Eva UntersmayrGiovanna BisesPhilipp StarklCharles L BevinsOtto ScheinerGeorge Boltz-NitulescuFritz WrbaErika Jensen-Jarolim<h4>Background</h4>IgE antibodies play a paramount role in the pathogenesis of various intestinal disorders. To gain insights in IgE-mediated pathophysiology of the gut, we investigated the expression of the high affinity IgE receptor Fc epsilonRI in human intestinal epithelium.<h4>Methodology/principal findings</h4>Fc epsilonRI alpha-chain, as detected by immunohistochemistry, was positive in epithelial cells for eight of eleven (8/11) specimens from colon cancer patients and 5/11 patients with inflammation of the enteric mucosa. The Fc epsilonRIalpha positive epithelial cells co-expressed Fc epsilonRIgamma, whereas with one exception, none of the samples was positive for the beta-chain in the epithelial layer. The functionality of Fc epsilonRI was confirmed in situ by human IgE binding. In experiments with human intestinal tumor cell lines, subconfluent Caco-2/TC7 and HCT-8 cells were found to express the alpha- and gamma-chains of Fc epsilonRI and to bind IgE, whereas confluent cells were negative for gamma-chains.<h4>Conclusions/significance</h4>Our data provide the first evidence that the components of a functional Fc epsilonRI are in vitro expressed by the human intestinal epithelial cells depending on differentiation and, more importantly, in situ in epithelia of patients with colon cancer or gastrointestinal inflammations. Thus, a contribution of Fc epsilonRI either to immunosurveillance or pathophysiology of the intestinal epithelium is suggested.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009023&type=printable |
| spellingShingle | Eva Untersmayr Giovanna Bises Philipp Starkl Charles L Bevins Otto Scheiner George Boltz-Nitulescu Fritz Wrba Erika Jensen-Jarolim The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. PLoS ONE |
| title | The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. |
| title_full | The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. |
| title_fullStr | The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. |
| title_full_unstemmed | The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. |
| title_short | The high affinity IgE receptor Fc epsilonRI is expressed by human intestinal epithelial cells. |
| title_sort | high affinity ige receptor fc epsilonri is expressed by human intestinal epithelial cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0009023&type=printable |
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