Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan
Objectives: Staphylococcus argenteus, a newly described species of coagulase-positive Staphylococcus, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of S....
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004454 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825199420975611904 |
|---|---|
| author | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Kenji Kudo Masahiko Ito Nobumichi Kobayashi |
| author_facet | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Kenji Kudo Masahiko Ito Nobumichi Kobayashi |
| author_sort | Meiji Soe Aung |
| collection | DOAJ |
| description | Objectives: Staphylococcus argenteus, a newly described species of coagulase-positive Staphylococcus, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of S. argenteus in the recent years. Methods: S. argenteus isolates from various clinical specimens were screened using MALDI-TOF and genotyped using the multilocus sequence typing scheme. Antimicrobial susceptibility was determined using broth microdilution test and resistance genes were detected by multiplex/uniplex PCR. Nucleotide sequences of haemolysin genes (hla, hlb, hld, and hlg) were determined and analysed in some isolates. Results: From August 2020 to December 2023, 210 isolates were identified as S. argenteus. The ratio of S. argenteus to Staphylococcus aureus clinical isolates was 0.0045, showing a slightly decreasing trend compared to what was previously reported (2019.8–2020.7). Three genotypes, ST1223-coa-XV, ST2198-coa-XIV, and ST2250-coa-XId were identified, with ST2250 being dominant (49%) and exhibiting an increasing trend. S. argenteus isolates were almost susceptible to antimicrobials examined, while ST2198 isolates showed higher resistance rates to ampicillin, macrolides, and aminoglycosides than other clones, harbouring blaZ, msrA, and aac(6′)-Ie-aph(2″)-Ia. Only eight isolates (4-ST2250, 3-ST1223, and 1-ST2198) did not show haemolysis on blood agar plates. These isolates had intact haemolysin genes and promoter regions; however, a novel deletion mutation in hlg-component A was detected in some haemolytic and non-haemolytic isolates. Conclusions: The present study revealed the epidemiological trend of S. argenteus clones in northern Japan, along with the occurrence of non-haemolytic isolates with intact haemolysin genes. |
| format | Article |
| id | doaj-art-695e0a45d6654c5c86986f23c20aa160 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-695e0a45d6654c5c86986f23c20aa1602025-02-08T05:00:24ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-01-014017Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern JapanMeiji Soe Aung0Noriko Urushibara1Mitsuyo Kawaguchiya2Nobuhide Ohashi3Mina Hirose4Kenji Kudo5Masahiko Ito6Nobumichi Kobayashi7Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan; Corresponding author. Mailing address: Department of Hygiene, Sapporo Medical University School of Medicine, S-1W-17, Chuo-ku, Sapporo 060-8556, Japan.Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, JapanDepartment of Oral Growth and Development, Division of Pediatric Dentistry, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, JapanSapporo Clinical Laboratory, Inc., Sapporo, JapanSapporo Clinical Laboratory, Inc., Sapporo, JapanDepartment of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, JapanObjectives: Staphylococcus argenteus, a newly described species of coagulase-positive Staphylococcus, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of S. argenteus in the recent years. Methods: S. argenteus isolates from various clinical specimens were screened using MALDI-TOF and genotyped using the multilocus sequence typing scheme. Antimicrobial susceptibility was determined using broth microdilution test and resistance genes were detected by multiplex/uniplex PCR. Nucleotide sequences of haemolysin genes (hla, hlb, hld, and hlg) were determined and analysed in some isolates. Results: From August 2020 to December 2023, 210 isolates were identified as S. argenteus. The ratio of S. argenteus to Staphylococcus aureus clinical isolates was 0.0045, showing a slightly decreasing trend compared to what was previously reported (2019.8–2020.7). Three genotypes, ST1223-coa-XV, ST2198-coa-XIV, and ST2250-coa-XId were identified, with ST2250 being dominant (49%) and exhibiting an increasing trend. S. argenteus isolates were almost susceptible to antimicrobials examined, while ST2198 isolates showed higher resistance rates to ampicillin, macrolides, and aminoglycosides than other clones, harbouring blaZ, msrA, and aac(6′)-Ie-aph(2″)-Ia. Only eight isolates (4-ST2250, 3-ST1223, and 1-ST2198) did not show haemolysis on blood agar plates. These isolates had intact haemolysin genes and promoter regions; however, a novel deletion mutation in hlg-component A was detected in some haemolytic and non-haemolytic isolates. Conclusions: The present study revealed the epidemiological trend of S. argenteus clones in northern Japan, along with the occurrence of non-haemolytic isolates with intact haemolysin genes.http://www.sciencedirect.com/science/article/pii/S2213716524004454Staphylococcus argenteusSTPrevalenceHaemolysinJapan |
| spellingShingle | Meiji Soe Aung Noriko Urushibara Mitsuyo Kawaguchiya Nobuhide Ohashi Mina Hirose Kenji Kudo Masahiko Ito Nobumichi Kobayashi Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan Journal of Global Antimicrobial Resistance Staphylococcus argenteus ST Prevalence Haemolysin Japan |
| title | Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan |
| title_full | Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan |
| title_fullStr | Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan |
| title_full_unstemmed | Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan |
| title_short | Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan |
| title_sort | prevalence and antimicrobial resistance of three clones st1223 st2198 st2250 of staphylococcus argenteus clinical isolates in northern japan |
| topic | Staphylococcus argenteus ST Prevalence Haemolysin Japan |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004454 |
| work_keys_str_mv | AT meijisoeaung prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT norikourushibara prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT mitsuyokawaguchiya prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT nobuhideohashi prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT minahirose prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT kenjikudo prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT masahikoito prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan AT nobumichikobayashi prevalenceandantimicrobialresistanceofthreeclonesst1223st2198st2250ofstaphylococcusargenteusclinicalisolatesinnorthernjapan |