Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm’s canal in mouse whole-mount corneal limbus tissue

Protocol for immunofluorescent staining, high-resolution imaging, and spatial projection of Schlemm’s canal in mouse whole-mount corneal limbus tissue

Summary: Schlemm’s canal (SC) is an endothelium-lined conduit with uniquely combined features of blood vessels and lymphatic vessels required for aqueous humor drainage from the eye. Here, we present a protocol for immunofluorescent staining of blood and lymphatic endothelial markers in mouse whole-...

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Bibliographic Details
Main Authors: Weifa Wang, Peiyao Peng, Wenfo Hu, Xuyang Liu, Liming Yang, Rong Ju, Feng Zhang
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
metabolism
microscopy
molecular biology
molecular/chemical probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003120
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