Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos
Objective: Radiofrequency electromagnetic field (RF-EMF) exposure during the embryonic period can cause defects in the development of the fetus. The study’s aim is to evaluate the effects of RF-EMF on the lipid accumulation, oxidant-antioxidant system parameters, locomotor activities, and gene expre...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2024-05-01
|
| Series: | European Journal of Biology |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/CBB293B0DFC84D34B29245AD0320F6FC |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849324325890424832 |
|---|---|
| author | Derya Cansız Merih Beler Gizem Eğilmezer Semanur Işıkoğlu Zülal Mızrak İsmail Ünal Selçuk Paker Ahmet Ata Alturfan Ebru Emekli Alturfan |
| author_facet | Derya Cansız Merih Beler Gizem Eğilmezer Semanur Işıkoğlu Zülal Mızrak İsmail Ünal Selçuk Paker Ahmet Ata Alturfan Ebru Emekli Alturfan |
| author_sort | Derya Cansız |
| collection | DOAJ |
| description | Objective: Radiofrequency electromagnetic field (RF-EMF) exposure during the embryonic period can cause defects in the development of the fetus. The study’s aim is to evaluate the effects of RF-EMF on the lipid accumulation, oxidant-antioxidant system parameters, locomotor activities, and gene expressions of insulin and leptin as genes related to insulin resistance in fetal hyperglycemia-induced zebrafish embryos. Materials and Methods: The study exposed zebrafish embryos to RF-EMF (60 min) and glucose (5%) every day until 96 hours post fertilization (hpf). The study measured lipid peroxidation (LPO), superoxide dismutase, nitric oxide (NO), glutathione S-transferase (GST), and glutathione (GSH) levels to observe the oxidative stress status. The study monitored the development of the zebrafish embryos under a microscope, performed a locomotor activity analysis, measured acetylcholinesterase activity, and conducted oil red O staining to determine lipid accumulation. The study used reverse transcription polymerase chain reactions (RT-PCRs) to determine the expressions of ins and lepa by using RT-PCR. Results: Both the glucose and RF-EMF applications decreased locomotor activity and increased the LPO and NO levels as oxidative damage indicators. Applying RF-EMF alone increased GST and GSH levels, while applying RF-EMF and glucose showed a decrease in the antioxidant defense systems. ins expression increased in the glucose and RF-EMF groups, while lepa expression increased in the glucose group and decreased in the RF-EMF group. Conclusion: The harmful effects of hyperglycemia and RF-EMF exposure during the fetal period on embryo development need to be supported by studies to confirm the changes the current study has identified at the gene and protein levels. |
| format | Article |
| id | doaj-art-69335eece63f4fa2be75a61ca6ac7cf0 |
| institution | Kabale University |
| issn | 2618-6144 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-69335eece63f4fa2be75a61ca6ac7cf02025-08-20T03:48:45ZengIstanbul University PressEuropean Journal of Biology2618-61442024-05-01831778410.26650/EurJBiol.2024.1467244123456Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish EmbryosDerya Cansız0https://orcid.org/0000-0002-6274-801XMerih Beler1https://orcid.org/0000-0002-3828-4630Gizem Eğilmezer2https://orcid.org/0000-0002-1231-5232Semanur Işıkoğlu3https://orcid.org/0009-0003-9779-2867Zülal Mızrak4https://orcid.org/0009-0004-7647-7267İsmail Ünal5https://orcid.org/0000-0002-8664-3298Selçuk Paker6https://orcid.org/0000-0002-1769-1835Ahmet Ata Alturfan7https://orcid.org/0000-0003-0528-9002Ebru Emekli Alturfan8https://orcid.org/0000-0003-2419-8587İstanbul Medipol Üniversitesi, Istanbul, TurkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, TürkiyeMarmara Üniversitesi, İstanbul, Türkiyeİstanbul Teknik Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi-Cerrahpaşa, Istanbul, TurkiyeMarmara Üniversitesi, İstanbul, TürkiyeObjective: Radiofrequency electromagnetic field (RF-EMF) exposure during the embryonic period can cause defects in the development of the fetus. The study’s aim is to evaluate the effects of RF-EMF on the lipid accumulation, oxidant-antioxidant system parameters, locomotor activities, and gene expressions of insulin and leptin as genes related to insulin resistance in fetal hyperglycemia-induced zebrafish embryos. Materials and Methods: The study exposed zebrafish embryos to RF-EMF (60 min) and glucose (5%) every day until 96 hours post fertilization (hpf). The study measured lipid peroxidation (LPO), superoxide dismutase, nitric oxide (NO), glutathione S-transferase (GST), and glutathione (GSH) levels to observe the oxidative stress status. The study monitored the development of the zebrafish embryos under a microscope, performed a locomotor activity analysis, measured acetylcholinesterase activity, and conducted oil red O staining to determine lipid accumulation. The study used reverse transcription polymerase chain reactions (RT-PCRs) to determine the expressions of ins and lepa by using RT-PCR. Results: Both the glucose and RF-EMF applications decreased locomotor activity and increased the LPO and NO levels as oxidative damage indicators. Applying RF-EMF alone increased GST and GSH levels, while applying RF-EMF and glucose showed a decrease in the antioxidant defense systems. ins expression increased in the glucose and RF-EMF groups, while lepa expression increased in the glucose group and decreased in the RF-EMF group. Conclusion: The harmful effects of hyperglycemia and RF-EMF exposure during the fetal period on embryo development need to be supported by studies to confirm the changes the current study has identified at the gene and protein levels.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/CBB293B0DFC84D34B29245AD0320F6FCradiofrequency electromagnetic fieldzebrafish embryosfetal hyperglycemiainsulin resistance |
| spellingShingle | Derya Cansız Merih Beler Gizem Eğilmezer Semanur Işıkoğlu Zülal Mızrak İsmail Ünal Selçuk Paker Ahmet Ata Alturfan Ebru Emekli Alturfan Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos European Journal of Biology radiofrequency electromagnetic field zebrafish embryos fetal hyperglycemia insulin resistance |
| title | Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos |
| title_full | Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos |
| title_fullStr | Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos |
| title_full_unstemmed | Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos |
| title_short | Radiofrequency Electromagnetic Field Exposure Amplifies the Detrimental Effects of Fetal Hyperglycemia in Zebrafish Embryos |
| title_sort | radiofrequency electromagnetic field exposure amplifies the detrimental effects of fetal hyperglycemia in zebrafish embryos |
| topic | radiofrequency electromagnetic field zebrafish embryos fetal hyperglycemia insulin resistance |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/CBB293B0DFC84D34B29245AD0320F6FC |
| work_keys_str_mv | AT deryacansız radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT merihbeler radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT gizemegilmezer radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT semanurisıkoglu radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT zulalmızrak radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT ismailunal radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT selcukpaker radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT ahmetataalturfan radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos AT ebruemeklialturfan radiofrequencyelectromagneticfieldexposureamplifiesthedetrimentaleffectsoffetalhyperglycemiainzebrafishembryos |