Prospective association between plasma amino acids and three Multimorbidity patterns in older adults

Abstract The role of metabolomic profiling on different multimorbidity patterns remains unknown. This study aims to assess the prospective relationship between plasma concentrations of amino acids and three different multimorbidity patterns: musculoskeletal and mental multimorbidity, cardiometabolic...

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Main Authors: Grace Velapatiño-Gamarra, Alberto Lana, Humberto Yévenes-Briones, Juan Cárdenas-Valladolid, José R. Banegas, Fernando Rodríguez-Artalejo, Esther López-García, Francisco Félix Caballero
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Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-06683-6
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author Grace Velapatiño-Gamarra
Alberto Lana
Humberto Yévenes-Briones
Juan Cárdenas-Valladolid
José R. Banegas
Fernando Rodríguez-Artalejo
Esther López-García
Francisco Félix Caballero
author_facet Grace Velapatiño-Gamarra
Alberto Lana
Humberto Yévenes-Briones
Juan Cárdenas-Valladolid
José R. Banegas
Fernando Rodríguez-Artalejo
Esther López-García
Francisco Félix Caballero
author_sort Grace Velapatiño-Gamarra
collection DOAJ
description Abstract The role of metabolomic profiling on different multimorbidity patterns remains unknown. This study aims to assess the prospective relationship between plasma concentrations of amino acids and three different multimorbidity patterns: musculoskeletal and mental multimorbidity, cardiometabolic multimorbidity, and cardiopulmonary multimorbidity. The study comprised a total of 1488 older adults from the Seniors-ENRICA 2 Spanish cohort. Plasma concentrations of alanine, glutamine, glycine, histidine, branched-chain amino acids, and aromatic amino acids were measured. Generalized estimating equation models were used to assess the prospective association between amino acids and multimorbidity patterns. Higher plasma concentrations of branched-chain amino acids as leucine [Odds Ratio (OR) per 1-SD increment = 1.05, 95% confidence interval (CI) = 1.01–1.08)], isoleucine (OR = 1.05, 95% CI = 1.01–1.08), and valine (OR = 1.04, 95% CI = 1.01–1.08) were related to cardiometabolic multimorbidity, while lower concentrations of glycine (OR = 0.95, 95% CI = 0.91–0.99) and tyrosine (OR = 0.96, 95% CI = 0.93–0.98) were also associated with the same multimorbidity pattern. On the other hand, higher plasma concentrations of glutamine (OR = 1.18, 95% CI = 1.03–1.34) were related to cardiopulmonary multimorbidity. In conclusion, branched-chain amino acids may serve as risk markers of cardiometabolic multimorbidity in older adults. Plasma concentrations of other amino acid species such as glycine, tyrosine, and glutamine could also help to identify cardiometabolic and cardiopulmonary multimorbidity patterns.
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spelling doaj-art-692e136723cb4daba73ee77077e0c4b62025-08-20T03:37:30ZengNature PortfolioScientific Reports2045-23222025-07-011511910.1038/s41598-025-06683-6Prospective association between plasma amino acids and three Multimorbidity patterns in older adultsGrace Velapatiño-Gamarra0Alberto Lana1Humberto Yévenes-Briones2Juan Cárdenas-Valladolid3José R. Banegas4Fernando Rodríguez-Artalejo5Esther López-García6Francisco Félix Caballero7Department of Preventive Medicine and Public Health, Universidad Autónoma de MadridDepartment of Medicine, Universidad de Oviedo/ISPADepartment of Preventive Medicine and Public Health, Universidad Autónoma de MadridDirección General de Investigación y Docencia, Consejería de Sanidad de la Comunidad de MadridDepartment of Preventive Medicine and Public Health, Universidad Autónoma de MadridDepartment of Preventive Medicine and Public Health, Universidad Autónoma de MadridDepartment of Preventive Medicine and Public Health, Universidad Autónoma de MadridDepartment of Preventive Medicine and Public Health, Universidad Autónoma de MadridAbstract The role of metabolomic profiling on different multimorbidity patterns remains unknown. This study aims to assess the prospective relationship between plasma concentrations of amino acids and three different multimorbidity patterns: musculoskeletal and mental multimorbidity, cardiometabolic multimorbidity, and cardiopulmonary multimorbidity. The study comprised a total of 1488 older adults from the Seniors-ENRICA 2 Spanish cohort. Plasma concentrations of alanine, glutamine, glycine, histidine, branched-chain amino acids, and aromatic amino acids were measured. Generalized estimating equation models were used to assess the prospective association between amino acids and multimorbidity patterns. Higher plasma concentrations of branched-chain amino acids as leucine [Odds Ratio (OR) per 1-SD increment = 1.05, 95% confidence interval (CI) = 1.01–1.08)], isoleucine (OR = 1.05, 95% CI = 1.01–1.08), and valine (OR = 1.04, 95% CI = 1.01–1.08) were related to cardiometabolic multimorbidity, while lower concentrations of glycine (OR = 0.95, 95% CI = 0.91–0.99) and tyrosine (OR = 0.96, 95% CI = 0.93–0.98) were also associated with the same multimorbidity pattern. On the other hand, higher plasma concentrations of glutamine (OR = 1.18, 95% CI = 1.03–1.34) were related to cardiopulmonary multimorbidity. In conclusion, branched-chain amino acids may serve as risk markers of cardiometabolic multimorbidity in older adults. Plasma concentrations of other amino acid species such as glycine, tyrosine, and glutamine could also help to identify cardiometabolic and cardiopulmonary multimorbidity patterns.https://doi.org/10.1038/s41598-025-06683-6Amino acidsMusculoskeletal and mental Multimorbidity patternCardiometabolic Multimorbidity patternCardiopulmonary Multimorbidity patternRisk markers
spellingShingle Grace Velapatiño-Gamarra
Alberto Lana
Humberto Yévenes-Briones
Juan Cárdenas-Valladolid
José R. Banegas
Fernando Rodríguez-Artalejo
Esther López-García
Francisco Félix Caballero
Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
Scientific Reports
Amino acids
Musculoskeletal and mental Multimorbidity pattern
Cardiometabolic Multimorbidity pattern
Cardiopulmonary Multimorbidity pattern
Risk markers
title Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
title_full Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
title_fullStr Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
title_full_unstemmed Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
title_short Prospective association between plasma amino acids and three Multimorbidity patterns in older adults
title_sort prospective association between plasma amino acids and three multimorbidity patterns in older adults
topic Amino acids
Musculoskeletal and mental Multimorbidity pattern
Cardiometabolic Multimorbidity pattern
Cardiopulmonary Multimorbidity pattern
Risk markers
url https://doi.org/10.1038/s41598-025-06683-6
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